Plant Growth Promoting Bacteria

The rhizosphere is a narrow region of soil that is directly influenced by root secretions and associated microbial activity [7]. Plant growth-promoting bacteria (PGPB) occupy the rhizosphere of many plant species and have beneficial effects on the host plant. They may influence the plant in a direct or indirect manner. A direct mechanism would be to increase plant growth by supplying the plant with nutrients and hormones; Indirect mechanisms on the otherhand, include, reduced susceptibility to diseases, and activating a form of defense referred to as induced systematic resistance (ISR) [5]. Examples of bacteria which have been found to enhance plant growth, include Pseudomonas, Enterobacter, and Arthrobacter [6].

Plant Growth Promotion

Nitrogen Fixation

Groundwater serves as water for more than 50% of people living in North America therefore a significant public resource. To date, major contamination of groundwater in North America are due to the release and use of chlorinated ethenes by industry. Examples of such toxic compounds are perchloroethene (PCE), trichloroethene (TCE). Carbon tetrachloride (CT) is also a major groundwater pollutant [4]. These compounds were widely used as solvents for dry cleaning and in textile manufacturing. They are sufficiently water soluble and can travel through soil where they reach the groundwater. The relative high concentration of them here can be harmful [6]. Ground water is also contaminated by pollutants that are not highly toxic, but can be utilized or modified by microorganisms to become more toxic. For instance over-fertilization in agriculture leads to an increased nitrate concentration which i.e. can cause the Blue Baby syndrome. This is seen in infants younger than six month old who rely on bacteria to digest their food. Some of these bacteria also convert nitrate, a component of fertilizer, to nitrite. In the blood nitrite reacts with hemoglobin interfering with its ability to carry oxygen. The babies show sign of suffocation and gets a bluish skin [2].

Microbial metabolism of groundwater pollutants

Co-metabolism and degradation of TCE

Some dehalorespiring organisms are capable of degrading PCE, TCE and CT into non-toxic compounds. Degradation of PCE is only known to happen through reductive dechlorination and only under anaerobic condition. TCE is, unlike PCE, able to be degraded under aerobic conditions. This can happen through cometabolism. In co-metabolism a compound is transformed by an organism that doesn’t use the compound as an energy or carbon source and reducing power is not provided. The organism relies on another compound to serve as an energy and carbon source [3] . Methanotrophic organisms grow on methane as a primary substrate and oxygen but some are also able to degrade TCE as a secondary substrate. This is because of nonspecific enzymatic activity of enzymes (methane monooxygenase, MMO) involved in degradation of the primary substrate. The degradation of TCE serves no beneficial purpose for these organisms. It generates an epoxide(cf. figure 1) which is transported out of the cell and here other heterotrophic organisms bring about the transformation into non-toxic compounds resulting in the formation of CO2. Several factors inhibit the aerobic degradation of TCE here among the concentration of contamination, the pH and the temperature. Because both TCE and methane bind to the same site in MMO competition between growth substrate and non-growth substrate also seems to limit degradation of TCE [3].

Dehalogenation

The bacterium Pseudomonas stutzeri strain KC can dehalogenate CT into carbon dioxide and chlorine without producing the toxic intermediate chloroform (CCl3H). This bacterium is originally isolated from an aquifer in Seal Beach in California. It is dependent on anaerobic conditions and in iron-limited media this bacterium produces and secretes a chelator called pyridine-2,6 (bis)thiocarboxylate (PDTC cf. figure 2.) [5]. When PDTC is in contact with a broad range of cell components it turns into a reduced form (the iron in the complex is reduced) and this is essential for its extracellular activity. PDCT has to be in a complex with copper in order for the fast turnover rate of CT into CO2. This complex functions both as a reactant and a catalyst in the reaction. When Pseudomonas stutzeri is in environments were nitrate is present as the electron acceptor a more rapid production of PDTC is observed [6].

Denitrification

In many agricultural areas in North America the nitrate concentrations exceed the standards. Denitrifying organisms are capable of using nitrate or nitrite as terminal electron acceptors thereby removing the excess of nitrogen from the environment. The organism Methylomirabilis oxyfera is an example of such an organism. This denitrifying bacterium is special in that it doesn’t have the gene encoding nitrous oxide reductase, the protein that converts N2O to N2. Instead they harbor an operon which encodes the complete methane monooxygenase complex. This enables it to oxide methane in an aerobic pathway [1]. The mechanism takes advances of the oxidation of methane to drive denitrification. They do so by producing oxygen from nitrite via nitrite oxide (thereby bypassing the intermediate nitrous oxide) and then use this oxygen to oxide methane in an anaerobic environment. This is called nitrite dependent anaerobic methane oxidation. The overall redox reaction is 3CH4 + 8NO2- + 8H+ -> 3CO2 + 4N2 + 10 H2O. In this way the organism uses the potent greenhouse gas methane and reduces nitrite thereby contributing to the removal of excess N-compounds in groundwater [1].

Treatment technologies

Contamination of groundwater can lead to severe health problems and environmental changes if left untreated. Bioaugmentation is a widespread biological technique used in the removal of chlorinated compounds. By introducing natural electron donors that are helpful in the removal of halogenated compounds into the groundwater the growth of dehalorespiring organisms can be favored. Optional conditions for dehalogenation are provided without any engineering steps taken [7]. Pump and treat method is also one of the most used groundwater remediation techniques. Removal of contaminated groundwater from soil with the use of pumps followed by subsequent remediation at the surface helps overcome the persistence of the pollutants (cf. figure 3). It is typically biological or chemical treatments that remove the pollutants. This method is costly and slow however and some contaminants cannot be removed because they stick to soil and rocks or are not sufficient water soluble [4].

References

[1] Glick, B. R. “Modulation of Plant Ethylene Levels by the Bacterial Enzyme ACC Deaminase.” FEMS Microbiology Letters, 2006, DOI: 10.1016/j.femsle.2005.07.030

[2] Lugtenberg, B. and Kamilova, F. “Plant-Growth-Promoting Rhizobacteria.” Annual Review of Microbiology, 2009, DOI: 10.1146/annurev.micro.62.081307.162918

[3] Van Loon, L. C. “Plant Responses to Plant Growth-Promoting Rhizobacteria.” European Journal of Plant Pathology, 2007, DOI: 1007/s10658-007-9165-1

[4] Vessey, J. K. “Plant Growth Promoting Rhizobacteria as Biofertilizers.” Plant and Soil, 2003, DOI: 10.1023/A:1026037216893

[5] Yang, J., Kloepper, J., and Ryu, C. “Rhizosphere Bacteria Helps Plants Tolerate Abiotic Stress.” Trends in Plant Science, 2009, DOI: 10.1016/j.tplants.2008.10.004

[6] Saharan, B. S. and Nehra, V. “Plant Growth Promoting Rhizobacteria: A Critical Review.” Life Sciences and Medicine Research, 2011

[7] Bloemberg, G. V. and Lugtenberg, B. “Molecular Basis of Plant Growth Promotion and Biocontrol by Rhizobacteria.” Current Opinion in Plant Biology, 2001, DOI: 10.1016/S1369-5266(00)00183-7

[8] Mantelin, S. and Touraine, B. “ Plant Growth-Promoting Bacteria and Nitrate Availability: Impacts on Root Development and Nitrate Uptake.” Journal of Experimental Botany, 2003, DOI: 10.1093/jxb/erh010

[9] Glick, B., Cheng, Z., Czarny, J., and Duan, J. “Promotion of Plant Growth by ACC Deaminase-Producing Soil Bacteria.” European Journal of Plant Pathology, 2007, DOI: 10.1007/s10658-007-9162-4

[10] Bashan, Y., Puente, M., de-Bashan, L., and Hernandez, J. P. “Environmental Uses of Plant Growth-Promoting Bacteria.” Plant-Microbe Interactions, 2008