Difference between revisions of "Poliomyelitis"

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(Virology and Taxonomy)
(Pathogenesis)
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==Pathogenesis==
 
==Pathogenesis==
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===Transmission===
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As a virus that is shed in feces even by asymptomatic persons, poliovirus passes from person to person through the fecal-oral route. Highly communicable, poliovirus tends to be passed to 90 percent of adults and 100 percent of children in a single household. The virus, however, is only infectious in humans. Upon infection, the virus will multiple in the oropharyngeal and intestinal mucosa before draining into the lymph nodes and into the blood. Most infections do not progress past this point and cause minor symptoms. A small amount of infections, however, do pass into the central nervous system and cause various degress of paralysis. Incubation times can vary depending on what symptoms emerge. Minor illnesses of the virus typically manifest themselves between three to five days while more serious symptoms related to CNS infection take one to two weeks.
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===Entering host cells===
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Poliovirus enters host cells by binding to its receptor identified as CD155, a glycoprotein of the immunoglobin superfamily. Once multiple virus particles bind the V-type domains of their receptors,  they viral particles undergo a conformational change which externalizes the proteins VP4 and VP1 found on the capsid. The proteins then insert into the cell membrane forming channels in the cell membrane. *** The pathway by which the viral particles are internalized in the cell is throught to depend on actin and tyrosine kinase. Shortly after endocytosis of the virus particle, the RNA genome is quickly released into the cytoplasm through a pore in the newly formed vesicle. As genome release occurs within only 100 to 200 nm of the cell surface, the event might be triggered as the resulting curvature of the forming vesicle inserts more VP1 N-termini into the cell membrane.
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===Dissemination into central nervous system===
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While the mechanism by which poliovirus enters the CNS remains undefined, research suggests two possible routes: passage through the blood-brain barrier and transport through neural pathways. Antiviral antibodies given intravenously to mice prevents infection of the brain and spinal cord suggesting that the resulting viremia plays a role in paralytic cases. In addition, the virus has been shown to travel up nerve cells exceeding 12 cm a day, and the molecular mechanism is thought to involve Tctex-1, a part of the retrograde motor complex. Upon entry into the cell, the vesicle containing the virus particle is thought to be connected to Tctex-1 by the cytoplasmic domain of CD155. This interaction allows the vesicle to be transported toward the cell body of the neuron where the RNA genome is released for replication. The process is repeated through each nerve cell to allow the virus to gradually progress toward the spinal cord and then the brain.
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==Clinical features==
 
==Clinical features==
 
==Diagnosis==
 
==Diagnosis==

Revision as of 10:27, 22 July 2013

This student page has not been curated.
Electron micrograph of poliovirus. From: Wikipedia Commons [1]
Poliovirus
Scientific classification
Group: Group IV
Order: Picornavirales
Family: Picornaviridae
Genus: Enterovirus
Species: Enterovirus C
Subtype: Poliovirus


Description

Poliomyelitis is viral disease caused by an enterovirus known as poliovirus and is well known for its role in causing paralysis, especially in infants. While most infections are asymptomatic, viral particles that gain entrance into the central nervous system can replicate in neurons and destroy cells that govern muscle function resulting in flaccid paralysis.

Epidemics involving the disease, also known as polio, have stuck the human race throughout history. The emergence of polio vaccines in the mid 20th century, however, has given public health organizations the tools needed to eradicate the disease. Worldwide immunization efforts have reduced worldwide cases from the hundreds of thousands to less than a thousand per year. With the Western Hemisphere and Europe declared polio-free in 1994 and 2002 respectively, the virus only remains endemic in Nigeria, Afghanistan and Pakistan. If successfully eradicated, polio will be one of only three diseases eradicated in history—the others being smallpox and rinderpest which were declared eradicated in 1979 and 2011 respectively.

Virology and Taxonomy

Poliovirus is a member of the picornavirus viral family, a taxonomic grouping that includes rhino viruses and hepatisis A virus. Picornaviruses are characterized by their icosahedral capsid structure that lacks a viral envelope and carries the single-strand RNA genome. Enterovirus, the genus that includes poliovirus, represents viruses that can withstand low pH and thus pass through the stomach to infect and replicate within intestinal epithelial cells. This ability allows poliovirus to be highly infectious through the fecal-oral route.

Genome and Structure

The virus’ approximately 7500 nucleotide RNA genome codes for the proteins that form its capsid as well as _______. As the genome is translated within the host cell, the polyprotein is cleaved to release P1, which codes for all the proteins needed to form the capsid. 3CD, a viral protease, cleaves P1 to release VP1, VP3 and myristoyl-VP0 which will form pentamers that will eventually create empty capsids made of 60 copies of each of the aforementioned proteins. Once the RNA genome has been encapsidated, the infectious virion will be formed with the cleavage of VP0 into VP2 and VP4 thus leaving the capsid structure complete with 60 copies each of the four proteins: VP1, VP2, VP3 and VP4.

Serotypes

Three distinct serotypes, PV1, PV2 and PV3, are associated with the paralytic disease with PV1 being associated most frequently with paralytic poliomyelitis.

Pathogenesis

Transmission

As a virus that is shed in feces even by asymptomatic persons, poliovirus passes from person to person through the fecal-oral route. Highly communicable, poliovirus tends to be passed to 90 percent of adults and 100 percent of children in a single household. The virus, however, is only infectious in humans. Upon infection, the virus will multiple in the oropharyngeal and intestinal mucosa before draining into the lymph nodes and into the blood. Most infections do not progress past this point and cause minor symptoms. A small amount of infections, however, do pass into the central nervous system and cause various degress of paralysis. Incubation times can vary depending on what symptoms emerge. Minor illnesses of the virus typically manifest themselves between three to five days while more serious symptoms related to CNS infection take one to two weeks.

Entering host cells

Poliovirus enters host cells by binding to its receptor identified as CD155, a glycoprotein of the immunoglobin superfamily. Once multiple virus particles bind the V-type domains of their receptors, they viral particles undergo a conformational change which externalizes the proteins VP4 and VP1 found on the capsid. The proteins then insert into the cell membrane forming channels in the cell membrane. *** The pathway by which the viral particles are internalized in the cell is throught to depend on actin and tyrosine kinase. Shortly after endocytosis of the virus particle, the RNA genome is quickly released into the cytoplasm through a pore in the newly formed vesicle. As genome release occurs within only 100 to 200 nm of the cell surface, the event might be triggered as the resulting curvature of the forming vesicle inserts more VP1 N-termini into the cell membrane.

Dissemination into central nervous system

While the mechanism by which poliovirus enters the CNS remains undefined, research suggests two possible routes: passage through the blood-brain barrier and transport through neural pathways. Antiviral antibodies given intravenously to mice prevents infection of the brain and spinal cord suggesting that the resulting viremia plays a role in paralytic cases. In addition, the virus has been shown to travel up nerve cells exceeding 12 cm a day, and the molecular mechanism is thought to involve Tctex-1, a part of the retrograde motor complex. Upon entry into the cell, the vesicle containing the virus particle is thought to be connected to Tctex-1 by the cytoplasmic domain of CD155. This interaction allows the vesicle to be transported toward the cell body of the neuron where the RNA genome is released for replication. The process is repeated through each nerve cell to allow the virus to gradually progress toward the spinal cord and then the brain.

Clinical features

Diagnosis

Treatment

Prevention

Host Immune Response

References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

Created by Jake Morgan, student of Tyrrell Conway at the University of Oklahoma.