Prevotella histicola: Difference between revisions

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|Species=histicola
|Species=histicola
|Species source text=Prevotella histicola [his.ti9co.la. N.L. masc. n. histus (from Gr. histos) tissue; L. suffix -cola (from incola) inhabitant; N.L. fem./masc. n. histicola inhabitant of tissue].
|Species source text=Prevotella histicola [his.ti9co.la. N.L. masc. n. histus (from Gr. histos) tissue; L. suffix -cola (from incola) inhabitant; N.L. fem./masc. n. histicola inhabitant of tissue].
|Strain=T05-04
|Strain=T05-04 (= DSM 19854 =CCUG 55407)
|Strain source text=The type strain of Prevotella histicola is T05-04T (5DSM 19854T5CCUG 55407T).
|Strain source text=he type strain of Prevotella histicola is T05-04T (5DSMT =CCUG 55407T).
|Genome size=0.7 X 0.8-3.0 um
|Genome size=0.7 X 0.8-3.0 um
|Genome size source text=Gram-negative bacilli that are 0.760.8–3.0 um in size.  
|Genome size source text=Gram-negative bacilli that are 0.7 X 0.8–3.0 um in size.
|Percent G plus C=43 mol%
|Percent G plus C=43 mol%
|Percent G plus C source text=The G+C content of the DNA of the type strain is 43 mol%.  
|Percent G plus C source text=The G+C content of the DNA of the type strain is 43 mol%.
|Cell shape=bacillus
|Cell shape=bacillus
|Cell shape source text=Gram-negative bacilli isolated from human oral
|Cell shape source text=Gram-negative bacilli isolated from human oral
|Pigments=variably
|Pigments=variably; cream and opaque
|Pigments source text=The results of the phenotypic tests can be viewed in table format as supplementary data (Supplementary Table S1) in IJSEM Online. Strains T05-04T, N12-20 and N19-30 were obligately anaerobic, non-motile, variably pigmenting, Gram-negative bacilli that were 0.7 mm wide by 0.8– 3.0 mm long (and occasionally up to 5 mm long). After 3 days incubation on FAA plates, colonies were 1.5– 2.0 mm in diameter, circular, entire, convex, cream and opaque with a solid non-translucent internal appearance when viewed under a plate microscope (Fig. 1a). Upon further incubation, strain N19-30 developed a deep reddish-brown pigment in the centre of the colony, forming a bull’s-eye appearance (Fig. 1b). Strains T05- 04T and N12-20 remained unpigmented even after 2 weeks incubation on FAA with 5 % rabbit blood. However, in the presence of a metronidazole disc (5 mg; Oxoid), strains T05-04T and N12-20 formed some black-pigmented colonies around the edge of the zone of growth inhibition on FAA (Fig. 1c). When these pigmented colonies were subcultured they retained their black pigmentation even in the absence of metronidazole (Fig. 1d).
|Pigments source text=Cells are obligately anaerobic, non-motile, variably pig- menting, Gram-negative bacilli that are 0.760.8–3.0 mm in size. After 3 days incubation on FAA plates, colonies are 1.5–2.0 mm in diameter, circular, entire, convex, cream- coloured and opaque. Some strains produce black colonies in the presence of metronidazole and other strains form bull’s-eye colonies with reddish-brown pigmentation in the centres. Growth in broth media produces moderate turbidity that is markedly enhanced by the addition of fermentable carbohydrates.  
|cell wall=Gram-negative
|cell wall=Gram-negative
|Cell wall source text=Gram-negative bacilli isolated from human oral
|Cell wall source text=Gram-negative bacilli isolated from human oral
|motility=non motile
|motility=non motile
|Motility source text=Cells are obligately anaerobic, non-motile, variably pig- menting,  
|Motility source text=Cells are obligately anaerobic, non-motile, variably pig- menting,
|habitat=mucosal tissue from the human oral cavity
|habitat=mucosal tissue from the human oral cavity
|Habitat source text=were isolated from mucosal tissue from the human oral cavity.
|Habitat source text=were isolated from mucosal tissue from the human oral cavity.
|oxygen use=anaerobic
|oxygen use=anaerobic
|Oxygen use source text=Cells are obligately anaerobic, non-motile, variably pig- menting,  
|Oxygen use source text=Cells are obligately anaerobic, non-motile, variably pig- menting,
|host=Human oral cavity
|mono & di-saccharides=fructose, glucose, lactose, maltose, mannose, sucrose
|Host source text=Prevotella species constitute part of the commensal oral microbiota in humans and are also associated with various oral diseases and infections at other body sites. The oral mucosa is heavily colonized with bacteria, and bacteria are known to invade the epithelial cells lining the cheeks (Rudney et al., 2005).
|mono & di-saccharides source text=Cells are saccharolytic and are able to ferment fructose, glucose, lactose, maltose, mannose, raffinose and sucrose, but not arabinose,cellobiose, mannitol, melezitose, melibiose, rhamnose, ribose, salicin, sorbitol, trehalose or xylose.
|polysaccharides=raffinose,  
|Fermentation products=acetic acid and succinct acid
|Fermentation products=acetic acid and succinct acid
|Fermentation products source text=Prevotella histicola is saccharolytic and produces acetic acid and succinic acid as major end products of fermentation and trace to minor amounts of isovaleric acid and lactic acid.  
|Fermentation products source text=Prevotella histicola is saccharolytic and produces acetic acid and succinic acid as major end products of fermentation and trace to minor amounts of isovaleric acid and lactic acid.
|Peer review ready=No
|Peer review ready=Yes
|Peer review complete=No
|Peer review complete=No
}}
}}

Revision as of 19:23, 6 March 2014


Source text
16S rRNA accession # IMG Genome ID:: [[]]
Family Family::Prevotellaceae [[]]
Genus Genus::Prevotella Phylogenetic analysis of the 16S rRNA gene sequence of strain T05-04T revealed this organism to belong to the genus Prevotella (Fig. 2)
Species Species::histicola [[Prevotella histicola [his.ti9co.la. N.L. masc. n. histus (from Gr. histos) tissue; L. suffix -cola (from incola) inhabitant; N.L. fem./masc. n. histicola inhabitant of tissue].]]
Strain Strain::T05-04 (= DSM 19854 =CCUG 55407) he type strain of Prevotella histicola is T05-04T (5DSMT =CCUG 55407T).

Genetic characteristics

Genome size: Genome size::0.7 X 0.8-3.0 um Gram-negative bacilli that are 0.7 X 0.8–3.0 um in size.
%G+C: Percent size::43 mol% The G+C content of the DNA of the type strain is 43 mol%.
Other genetic characteristics: [[Other genetic characteristics::{{{Other genetic characteristics}}}]] [[{{{Other genetic characteristics source text}}}]]

Morphological features

Cell shape Cell shape::bacillus Gram-negative bacilli isolated from human oral
Pigments Pigments::variably; cream and opaque [[Cells are obligately anaerobic, non-motile, variably pig- menting, Gram-negative bacilli that are 0.760.8–3.0 mm in size. After 3 days incubation on FAA plates, colonies are 1.5–2.0 mm in diameter, circular, entire, convex, cream- coloured and opaque. Some strains produce black colonies in the presence of metronidazole and other strains form bull’s-eye colonies with reddish-brown pigmentation in the centres. Growth in broth media produces moderate turbidity that is markedly enhanced by the addition of fermentable carbohydrates.]]
Cell wall Cell Wall::Gram-negative Gram-negative bacilli isolated from human oral
Motility motility::non motile Cells are obligately anaerobic, non-motile, variably pig- menting,
Biofilm formation biofilm formation:: [[]]

Environmental parameters

Habitat isolated from habitat::mucosal tissue from the human oral cavity were isolated from mucosal tissue from the human oral cavity.
Oxygen use oxygen use::anaerobic Cells are obligately anaerobic, non-motile, variably pig- menting,
Salinity preference salinity preference:: [[]]
pH minimum pH minimum::
pH optimum pH optimum::
pH maximum pH maximum:: [[]]
Temperature minimum Temperature minimum::
Temperature optimum Temperature optimum::
Temperature maximum Temperature maximum:: [[]]
NaCl minimum NaCl minimum::
NaCl optimum NaCl optimum::
NaCl maximum NaCl maximum:: [[]]

Host microbe interactions

Host host:: [[]]
Symbiotic symbiotic:: [[]]
Pathogenic pathogenic:: [[]]
Disease caused Causes disease::
Metabolism (energy & carbon source)
Mono & di-saccharides mono & di-saccharides::fructose, glucose, lactose, maltose, mannose, sucrose Cells are saccharolytic and are able to ferment fructose, glucose, lactose, maltose, mannose, raffinose and sucrose, but not arabinose,cellobiose, mannitol, melezitose, melibiose, rhamnose, ribose, salicin, sorbitol, trehalose or xylose.
Polysaccharides Polysaccharides::raffinose, [[]]
Amino acids Amino acids:: [[]]
Alcohols Alcohols:: [[]]
Fatty acids Fatty acids:: [[]]
Other energy or carbon sources MetabolizesOther:: [[]]

Metabolite products

Fermentation products Fermentation products::acetic acid and succinct acid Prevotella histicola is saccharolytic and produces acetic acid and succinic acid as major end products of fermentation and trace to minor amounts of isovaleric acid and lactic acid.
Polyalkanoates (plastics) Polyalkanoates:: [[]]
Other metabolic product Other metabolic product:: [[]]

Antibiotic characteristics

Antibiotic sensitivity Antibiotic sensitivity:: [[]]
Antibiotic resistant Antibiotic resistant:: [[]]

Peer review ready: Peer review ready::Yes Peer review complete: Peer review complete::No