Pseudomonas denitrificans: Difference between revisions

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Include this section if your Wiki page focuses on a specific taxon/group of organisms
Include this section if your Wiki page focuses on a specific taxon/group of organisms
=2. Description and significance=
=2. Description and significance=
<nowiki>Pseudomonas denitrificans is a polar flagellated, rod-shaped, Gram-negative, aerobic, heterotrophic bacteria species with the ability to produce vitamin B12.1  P. denitrificans is one of the few microorganisms that can synthesize vitamin B12 under aerobic conditions.2 As the name suggests, P. denitrificans is also capable of performing denitrification as a part of nitrogen cycle, a process in which nitrate is reduced into nitrogen gas (N2).2
''Pseudomonas denitrificans'' is a polar flagellated, rod-shaped, Gram-negative, aerobic, heterotrophic bacteria species with the ability to produce vitamin B12.1  ''P. denitrificans'' is one of the few microorganisms that can synthesize vitamin B12 under aerobic conditions.2 As the name suggests, ''P. denitrificans'' is also capable of performing denitrification as a part of nitrogen cycle, a process in which nitrate is reduced into nitrogen gas (N2).2


Despite the enormous knowledge known about of P. denitrificans, there is still a lot of information unknown. Its cytochrome cc’ protein, which is found in the mitochondria, is essential in the electron transport chain, but yet to be studied in depth for its relationship to similar proteins in  photosynthetic bacteria.3 The evolutionary implications of conserved genes encoding for vitamin B12 production may yet reveal insights into the origin of metabolism, since the pathway is thought to have developed to support fermentation processes, but as of yet not conclusively proven.4 P. denitrificans’ medical and environmental significance, in terms of its industrial use for vitamin B12 production and potential nitrate toxicity or wastewater treatment applications, is also unavailable (Xia, Cusanovich, Rodionov, Martens, Paranova-Mancheva).(3-7)  
Despite the enormous knowledge known about of ''P. denitrificans'', there is still a lot of information unknown. Its cytochrome cc’ protein, which is found in the mitochondria, is essential in the electron transport chain, but yet to be studied in depth for its relationship to similar proteins in  photosynthetic bacteria.3 The evolutionary implications of conserved genes encoding for vitamin B12 production may yet reveal insights into the origin of metabolism, since the pathway is thought to have developed to support fermentation processes, but as of yet not conclusively proven.4 ''P. denitrificans''’ medical and environmental significance, in terms of its industrial use for vitamin B12 production and potential nitrate toxicity or wastewater treatment applications, is also unavailable (Xia, Cusanovich, Rodionov, Martens, Paranova-Mancheva).(3-7)  


Studying P. denitrificans can offer valuable insights because it is believed to be phylogenetically ancient, and so an opportunity for understanding metabolic evolution.4 Its vitamin B12­ production can provide nutritional services to many life forms.4 In terms of more fundamental significance, its chemotrophic use of the cytochrome cc’ heme contrasts with purple bacteria photosynthetic use of a structurally and genetically similar protein, which indicates that it exists at a critical juncture in ATP production evolution.3 Its supplementation of oxidative phosphorylation with denitrification provides insights into how it fulfills and maintains a niche across fluctuating O and N levels in environments.8
Studying ''P. denitrificans'' can offer valuable insights because it is believed to be phylogenetically ancient, and so an opportunity for understanding metabolic evolution.4 Its vitamin B12­ production can provide nutritional services to many life forms.4 In terms of more fundamental significance, its chemotrophic use of the cytochrome cc’ heme contrasts with purple bacteria photosynthetic use of a structurally and genetically similar protein, which indicates that it exists at a critical juncture in ATP production evolution.3 Its supplementation of oxidative phosphorylation with denitrification provides insights into how it fulfills and maintains a niche across fluctuating O and N levels in environments.8


Vitamin B12 production may also provide health services to humans, since it is a precursor to methionine synthase, responsible for regeneration of essential amino acids in humans and (R)-methylmalonyl-CoA, responsible for fatty acid catabolism.4 P. denitrificans may also be engineered to produce other commercial compounds, such as 3-hydroxypropionic acid.9.Its denitrification abilities have critical potential in wastewater management.7 Pathologically speaking, P. denitrificans may opportunistically cause meningitis in humans.10 P. denitrificans may also colonize the intestines of fish.11</nowiki>
Vitamin B12 production may also provide health services to humans, since it is a precursor to methionine synthase, responsible for regeneration of essential amino acids in humans and (R)-methylmalonyl-CoA, responsible for fatty acid catabolism.4 ''P. denitrificans'' may also be engineered to produce other commercial compounds, such as 3-hydroxypropionic acid.9.Its denitrification abilities have critical potential in wastewater management.7 Pathologically speaking, ''P. denitrificans'' may opportunistically cause meningitis in humans.10 ''P. denitrificans'' may also colonize the intestines of fish.11


=3. Genome structure=
=3. Genome structure=

Revision as of 21:43, 28 November 2018

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1. Classification

a. Higher order taxa

Domain; Phylum; Class; Order; Family; Genus Include this section if your Wiki page focuses on a specific taxon/group of organisms

2. Description and significance

Pseudomonas denitrificans is a polar flagellated, rod-shaped, Gram-negative, aerobic, heterotrophic bacteria species with the ability to produce vitamin B12.1 P. denitrificans is one of the few microorganisms that can synthesize vitamin B12 under aerobic conditions.2 As the name suggests, P. denitrificans is also capable of performing denitrification as a part of nitrogen cycle, a process in which nitrate is reduced into nitrogen gas (N2).2

Despite the enormous knowledge known about of P. denitrificans, there is still a lot of information unknown. Its cytochrome cc’ protein, which is found in the mitochondria, is essential in the electron transport chain, but yet to be studied in depth for its relationship to similar proteins in photosynthetic bacteria.3 The evolutionary implications of conserved genes encoding for vitamin B12 production may yet reveal insights into the origin of metabolism, since the pathway is thought to have developed to support fermentation processes, but as of yet not conclusively proven.4 P. denitrificans’ medical and environmental significance, in terms of its industrial use for vitamin B12 production and potential nitrate toxicity or wastewater treatment applications, is also unavailable (Xia, Cusanovich, Rodionov, Martens, Paranova-Mancheva).(3-7)

Studying P. denitrificans can offer valuable insights because it is believed to be phylogenetically ancient, and so an opportunity for understanding metabolic evolution.4 Its vitamin B12­ production can provide nutritional services to many life forms.4 In terms of more fundamental significance, its chemotrophic use of the cytochrome cc’ heme contrasts with purple bacteria photosynthetic use of a structurally and genetically similar protein, which indicates that it exists at a critical juncture in ATP production evolution.3 Its supplementation of oxidative phosphorylation with denitrification provides insights into how it fulfills and maintains a niche across fluctuating O and N levels in environments.8

Vitamin B12 production may also provide health services to humans, since it is a precursor to methionine synthase, responsible for regeneration of essential amino acids in humans and (R)-methylmalonyl-CoA, responsible for fatty acid catabolism.4 P. denitrificans may also be engineered to produce other commercial compounds, such as 3-hydroxypropionic acid.9.Its denitrification abilities have critical potential in wastewater management.7 Pathologically speaking, P. denitrificans may opportunistically cause meningitis in humans.10 P. denitrificans may also colonize the intestines of fish.11

3. Genome structure

Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence?

4. Cell structure

Interesting features of cell structure. Can be combined with “metabolic processes”

5. Metabolic processes

Describe important sources of energy, electrons, and carbon (i.e. trophy) for the organism/organisms you are focusing on, as well as important molecules it/they synthesize(s).

6. Ecology

Habitat; symbiosis; contributions to the environment.

7. Pathology

How does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.

8. Current Research

Include information about how this microbe (or related microbes) are currently being studied and for what purpose

9. References

It is required that you add at least five primary research articles (in same format as the sample reference below) that corresponds to the info that you added to this page. [Sample reference] Faller, A., and Schleifer, K. "Modified Oxidase and Benzidine Tests for Separation of Staphylococci from Micrococci". Journal of Clinical Microbiology. 1981. Volume 13. p. 1031-1035.