Difference between revisions of "Pseudomonas granadensis"

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(Physiology and Pathogenesis)
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==Physiology and Pathogenesis==
 
==Physiology and Pathogenesis==
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).<br>
+
<b>BIOCHEMICAL TEST RESULTS</b>
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br>
+
*<b>Phenol Red Broth Tests:</b> <i>Glucose:</i> positive; <i>Lactose:</i> negative; <i>Sucrose:</i> negative
 
+
*<b>Starch Hydrolysis Test:</b> negative
BIOCHEMICAL TEST RESULTS
+
*<b>Casein Hydrolysis Test:</b> negative
 
+
*<b>Gelatin Hydrolysis Test:</b> positive
Phenol Red Broth Tests: Glucose: positive; Lactose: negative; Sucrose: negative
+
*<b>DNA Hydrolysis Test:</b> slight positive
Starch Hydrolysis Test: positive
+
*<b>Lipid Hydrolysis Test:</b> positive
Casein Hydrolysis Test: negative
+
*<b>Methyl Red Test:</b> negative
Gelatin Hydrolysis Test: positive
+
*<b>Voges Proskauer Test:</b> negative
DNA Hydrolysis Test: slight positive
+
*<b>Citrate Test:</b> positive
Lipid Hydrolysis Test: positive
+
*<b>SIM Tests:</b> negative for all
Methyl Red Test: negative
+
*<b>Nitrate Reduction:</b> negative
Voges Proskauer Test: negative
+
*<b>Urea Hydrolysis:</b> negative
Citrate Test: positive
+
*<b>Triple Sugar Iron Agar:</b> negative for all
SIM Tests: negative for all
+
*<b>Oxidase Test:</b> negative
Nitrate Reduction: negative
+
*<b>Eosin Methylene Blue Agar (EMB) Test:</b> positive
Urea Hydrolysis: negative
+
*<b>Hektoen Enteric Agar (HE) Test:</b> negative
Triple Sugar Iron Agar: Glucose: negative; Lactose: negative; Sucrose: negative
+
*<b>MacConkey Agar Test:</b> negative
Oxidase Test: negative
+
*<b>Decarboxylation Tests:</b> <i>Arginine:</i> no change; <i>Lysine:</i> negative; <i>Orinithine:</i> negative
Eosin Methylene Blue Agar (EMB) Test: positive
+
*<b> Phenylalanine Deaminase Test:</b> negative
Hektoen Enteric Agar (HE) Test: negative
+
*<b> Catalase Test:</b> negative
MacConkey Agar Test: negative
+
*<b> Blood Agar Test:</b> slight positive
Decarboxylation Tests: Arginine: no change; Lysine: negative; Orinithine: negative
+
*<b>Mannitol Salt Agar (MSA) Test:</b> negative
Phenylalanine Deaminase Test: negative
+
*<b>Phenylethyl Alcohol Agar (PEA) Test:</b> negative
Catalase Test: negative
+
*<b>Bacitracin/Optochin Susceptibility Test:</b> <i>Bacitracin:</i> resistant; <i>Optochin:</i> resistant
Blood Agar Test: slight positive
+
*<b>Bile Esculin Test: </b> negative
Mannitol Salt Agar (MSA) Test: negative
+
*<b>6.5% Salt Tolerance Test: </b>slight positive
Phenylethyl Alcohol Agar (PEA) Test: negative
 
Bile Esculin Test: negative
 
6.5% Salt Tolerance Test: slight positive
 
Bacitracin/Optochin Susceptibility Test: Bacitracin: resistant; Optochin: resistant
 
  
  

Revision as of 19:41, 11 April 2018

This student page has not been curated.

Classification

Domain: Bacteria

Phylum: Proteobacteria

Class: Gammaproteobacteria

Order: Pseudomonadales

Family: Pseudomonadaceae

Genus: Pseudomonas

Other Names: › DSM 28040 › LMG 27940 › Pseudomonas granadensis Pascual et al. 2015 › Pseudomonas sp. F-278,770 › strain F-278,770

Species

NCBI: Taxonomy

Genus species Pseudomonas granadensis

Habitat Information

Latitude: 30.26 degrees Longitude: 97.69 degrees

It was a clear day with a temperature of 64 degrees in the area of Govalle, East Austin on January 25th 2018. A ziplock bag was used to collect the soil from the area mostly from the surface about one inch deep. The location of the soil sample chosen was in a neighborhood field often frequented by dogs. Rainfall was 0.0" and the pressure was 35.35". The description of the location was mostly Bergstrom soils and urban land. 0-2% slopes and rarely flooded.

Description and Significance

Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.


Cellular: gram negative aerobic

Colonial:

Genome Structure

Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.


Cell Structure, Metabolism and Life Cycle

Interesting features of cell structure; how it gains energy; what important molecules it produces.


Physiology and Pathogenesis

BIOCHEMICAL TEST RESULTS

  • Phenol Red Broth Tests: Glucose: positive; Lactose: negative; Sucrose: negative
  • Starch Hydrolysis Test: negative
  • Casein Hydrolysis Test: negative
  • Gelatin Hydrolysis Test: positive
  • DNA Hydrolysis Test: slight positive
  • Lipid Hydrolysis Test: positive
  • Methyl Red Test: negative
  • Voges Proskauer Test: negative
  • Citrate Test: positive
  • SIM Tests: negative for all
  • Nitrate Reduction: negative
  • Urea Hydrolysis: negative
  • Triple Sugar Iron Agar: negative for all
  • Oxidase Test: negative
  • Eosin Methylene Blue Agar (EMB) Test: positive
  • Hektoen Enteric Agar (HE) Test: negative
  • MacConkey Agar Test: negative
  • Decarboxylation Tests: Arginine: no change; Lysine: negative; Orinithine: negative
  • Phenylalanine Deaminase Test: negative
  • Catalase Test: negative
  • Blood Agar Test: slight positive
  • Mannitol Salt Agar (MSA) Test: negative
  • Phenylethyl Alcohol Agar (PEA) Test: negative
  • Bacitracin/Optochin Susceptibility Test: Bacitracin: resistant; Optochin: resistant
  • Bile Esculin Test: negative
  • 6.5% Salt Tolerance Test: slight positive


References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

Author

Page authored by Diane Marques and Emma Riegle, students of Prof. Kristine Hollingsworth at Austin Community College.