Pseudomonas granadensis

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Classification

Domain: Bacteria

Phylum: Proteobacteria

Class: Gammaproteobacteria

Order: Pseudomonadales

Family: Pseudomonadaceae

Genus: Pseudomonas

Other Names: › DSM 28040 › LMG 27940 › Pseudomonas granadensis Pascual et al. 2015 › Pseudomonas sp. F-278,770 › strain F-278,770

Species

NCBI: Taxonomy

Genus species Pseudomonas granadensis

Habitat Information

Latitude: 30.26 degrees Longitude: 97.69 degrees

It was a clear day with a temperature of 64 degrees in the area of Govalle, East Austin on January 25th 2018. A ziplock bag was used to collect the soil from the area mostly from the surface about one inch deep. The location of the soil sample chosen was in a neighborhood field often frequented by dogs. Rainfall was 0.0" and the pressure was 35.35". The description of the location was mostly Bergstrom soils and urban land. 0-2% slopes and rarely flooded.

Description and Significance

Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.

Cellular: gram negative aerobic

Colonial:

Genome Structure

Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.

Cell Structure, Metabolism and Life Cycle

Interesting features of cell structure; how it gains energy; what important molecules it produces.

Physiology and Pathogenesis

BIOCHEMICAL TEST RESULTS

• Phenol Red Broth Tests: Glucose: positive; Lactose: negative; Sucrose: negative
• Starch Hydrolysis Test: negative
• Casein Hydrolysis Test: negative
• Gelatin Hydrolysis Test: positive
• DNA Hydrolysis Test: slight positive
• Lipid Hydrolysis Test: positive
• Methyl Red Test: negative
• Voges Proskauer Test: negative
• Citrate Test: positive
• SIM Tests: negative for all
• Nitrate Reduction: negative
• Urea Hydrolysis: negative
• Triple Sugar Iron Agar: negative for all
• Oxidase Test: negative
• Eosin Methylene Blue Agar (EMB) Test: positive
• Hektoen Enteric Agar (HE) Test: negative
• MacConkey Agar Test: negative
• Decarboxylation Tests: Arginine: no change; Lysine: negative; Orinithine: negative
• Phenylalanine Deaminase Test: negative
• Catalase Test: negative
• Blood Agar Test: slight positive
• Mannitol Salt Agar (MSA) Test: negative
• Phenylethyl Alcohol Agar (PEA) Test: negative
• Bacitracin/Optochin Susceptibility Test: Bacitracin: resistant; Optochin: resistant
• Bile Esculin Test: negative
• 6.5% Salt Tolerance Test: slight positive

References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

Author

Page authored by Diane Marques and Emma Riegle, students of Prof. Kristine Hollingsworth at Austin Community College.