Pseudomonas granadensis

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Classification

Domain: Bacteria

Phylum: Proteobacteria

Class: Gammaproteobacteria

Order: Pseudomonadales

Family: Pseudomonadaceae

Genus: Pseudomonas

Other Names: › DSM 28040 › LMG 27940 › Pseudomonas granadensis Pascual et al. 2015 › Pseudomonas sp. F-278,770 › strain F-278,770

Species

NCBI: Taxonomy

Genus species Pseudomonas granadensis

Habitat Information

Latitude: 30.26 degrees Longitude: 97.69 degrees

It was a clear day with a temperature of 64 degrees in the area of Govalle, East Austin on January 25th 2018. A ziplock bag was used to collect the soil from the area mostly from the surface about one inch deep. The location of the soil sample chosen was in a neighborhood field often frequented by dogs. Rainfall was 0.0" and the pressure was 35.35". The description of the location was mostly Bergstrom soils and urban land. 0-2% slopes and rarely flooded.

Description and Significance

Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.


Cellular: gram negative aerobic

Colonial:

Genome Structure

Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.


Cell Structure, Metabolism and Life Cycle

Interesting features of cell structure; how it gains energy; what important molecules it produces.


Physiology and Pathogenesis

Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.

BIOCHEMICAL TEST RESULTS

Phenol Red Broth Tests: Glucose: positive; Lactose: negative; Sucrose: negative Starch Hydrolysis Test: positive Casein Hydrolysis Test: negative Gelatin Hydrolysis Test: positive DNA Hydrolysis Test: slight positive Lipid Hydrolysis Test: positive Methyl Red Test: negative Voges Proskauer Test: negative Citrate Test: positive SIM Tests: negative for all Nitrate Reduction: negative Urea Hydrolysis: negative Triple Sugar Iron Agar: Glucose: negative; Lactose: negative; Sucrose: negative Oxidase Test: negative Eosin Methylene Blue Agar (EMB) Test: positive Hektoen Enteric Agar (HE) Test: negative MacConkey Agar Test: negative Decarboxylation Tests: Arginine: no change; Lysine: negative; Orinithine: negative Phenylalanine Deaminase Test: negative Catalase Test: negative Blood Agar Test: slight positive Mannitol Salt Agar (MSA) Test: negative Phenylethyl Alcohol Agar (PEA) Test: negative Bile Esculin Test: negative 6.5% Salt Tolerance Test: slight positive Bacitracin/Optochin Susceptibility Test: Bacitracin: resistant; Optochin: resistant

References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

Author

Page authored by Diane Marques and Emma Riegle, students of Prof. Kristine Hollingsworth at Austin Community College.