Pseudomonas granadensis

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Domain: Bacteria

Phylum: Proteobacteria

Class: Gammaproteobacteria

Order: Pseudomonadales

Family: Pseudomonadaceae

Genus: Pseudomonas

Other Names: › DSM 28040 › LMG 27940 › Pseudomonas granadensis Pascual et al. 2015 › Pseudomonas sp. F-278,770 › strain F-278,770


NCBI: Taxonomy

Genus species Pseudomonas granadensis

Habitat Information

Latitude: 30.26 degrees Longitude: 97.69 degrees

It was a clear day with a temperature of 64 degrees in the area of Govalle, East Austin on January 25th 2018. A ziplock bag was used to collect the soil from the area mostly from the surface about one inch deep. The location of the soil sample chosen was in a neighborhood field often frequented by dogs. Rainfall was 0.0" and the pressure was 35.35". The description of the location was mostly Bergstrom soils and urban land. 0-2% slopes and rarely flooded.

Description and Significance

Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.

Cellular: gram negative aerobic


Genome Structure

Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.

Cell Structure, Metabolism and Life Cycle

Interesting features of cell structure; how it gains energy; what important molecules it produces.

Physiology and Pathogenesis

Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.


Phenol Red Broth Tests: Glucose: positive; Lactose: negative; Sucrose: negative Starch Hydrolysis Test: positive Casein Hydrolysis Test: negative Gelatin Hydrolysis Test: positive DNA Hydrolysis Test: slight positive Lipid Hydrolysis Test: positive Methyl Red Test: negative Voges Proskauer Test: negative Citrate Test: positive SIM Tests: negative for all Nitrate Reduction: negative Urea Hydrolysis: negative Triple Sugar Iron Agar: Glucose: negative; Lactose: negative; Sucrose: negative Oxidase Test: negative Eosin Methylene Blue Agar (EMB) Test: positive Hektoen Enteric Agar (HE) Test: negative MacConkey Agar Test: negative Decarboxylation Tests: Arginine: no change; Lysine: negative; Orinithine: negative Phenylalanine Deaminase Test: negative Catalase Test: negative Blood Agar Test: slight positive Mannitol Salt Agar (MSA) Test: negative Phenylethyl Alcohol Agar (PEA) Test: negative Bile Esculin Test: negative 6.5% Salt Tolerance Test: slight positive Bacitracin/Optochin Susceptibility Test: Bacitracin: resistant; Optochin: resistant


[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.


Page authored by Diane Marques and Emma Riegle, students of Prof. Kristine Hollingsworth at Austin Community College.