Pseudomonas oryzihabitans: Difference between revisions

From MicrobeWiki, the student-edited microbiology resource
Line 14: Line 14:


=3. Genome structure=
=3. Genome structure=
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence?
The genome of two strains of Pseudomonas oryzihabitans, AXSa06 and AXSa07, have been sequenced (9). The genome of the P. oryzihabitans strain AXSa06 has 5,109,344 base pairs in total and the AXSa07 strain has 4,666,115 base pairs. The two strains share a similar % G+C content, both around 66% of the whole genome (9). In the AXSa07 strain genome, there are 4495 protein coding sequences. However, the AXSa07 strain has 15 rRNA genes, higher than the AXSa06 strain, which has 5 rRNA genes (9).
A key difference between the AXSa06 strain and the AXSa07 strain lies in their ACC deaminase activity. ACC deaminase activity is often used by soil microorganisms to enhance plant growth by inhibiting the enzyme 1-aminocyclopropane-1-carboxylic acid (ACC), which is a precursor to ethylene, a hormone that controls plant maturation. The P. oryzihabitans strain AXSa06 has genes coding for ACC deaminase activity, while AXSa07 strain does not (9). In the P. oryzihabitans strain AXSa06 genome, there are 4929 protein coding sequences (9).
Besides AXSa06 and AXSa07 strains, the genome of GCMCC 6169, a soil strain of P. oryzihabitans, was shown to encode the protein POEP1 which aids cellulose breakdown. Although POEP1 does not break down cellulose directly, it synergistically enhances the activity of cellulase, a protein that directly hydrolyzes cellulose for energy (10).
 
=4. Cell structure=
=4. Cell structure=
Interesting features of cell structure. Can be combined with “metabolic processes”
Interesting features of cell structure. Can be combined with “metabolic processes”

Revision as of 14:28, 6 December 2021

This student page has not been curated.

1. Classification

a. Higher order taxa

Domain: Bacteria Phylum: Proteobacteria Class: Gammaproteobacteria Order: Pseudomonadales Family: Pseudomonadaceae Genus: Pseudomonas Species: Pseudomonas oryzihabitans

2. Introduction

Pseudomonas oryzihabitans is a rod-shaped, Gram-negative bacterium known to cause infections in humans, in both immunocompromised and non-immunocompromised individuals. Some infections that P. oryzihabitans can cause include nosocomial (hospital-acquired) sepsis, bacteremia, endophthalmitis, and peritonitis (1). In other unique cases, P. oryzihabitans has been documented to cause urinary tract infections and green nail syndrome (2, 3). P. oryzihabitans is commonly found in hospital settings, on respiratory equipment and unsterilized medical tools (1). In nature, P. oryzihabitans can be found in moist environments, like soil, rice paddies, running or standing water, and groundwater (4,5). In these environments, P. oryzihabitans has potential to promote root growth via suppressing parasitic nematode populations, making it easier to grow and maintain certain crops (6). Additionally, P. oryzihabitans has a positive growth effect on members of the cucurbit family affected by Bacterial Fruit Blotch disease (BFB) (7). P. oryzihabitans also negatively impacts the growth of Muskmelon by causing stem and leaf rot (8). Overall, in this paper we discuss how P. oryzihabitans is a multifaceted organism that plays various roles ecologically, agriculturally, and medically that has not been compiled previously.

3. Genome structure

The genome of two strains of Pseudomonas oryzihabitans, AXSa06 and AXSa07, have been sequenced (9). The genome of the P. oryzihabitans strain AXSa06 has 5,109,344 base pairs in total and the AXSa07 strain has 4,666,115 base pairs. The two strains share a similar % G+C content, both around 66% of the whole genome (9). In the AXSa07 strain genome, there are 4495 protein coding sequences. However, the AXSa07 strain has 15 rRNA genes, higher than the AXSa06 strain, which has 5 rRNA genes (9). A key difference between the AXSa06 strain and the AXSa07 strain lies in their ACC deaminase activity. ACC deaminase activity is often used by soil microorganisms to enhance plant growth by inhibiting the enzyme 1-aminocyclopropane-1-carboxylic acid (ACC), which is a precursor to ethylene, a hormone that controls plant maturation. The P. oryzihabitans strain AXSa06 has genes coding for ACC deaminase activity, while AXSa07 strain does not (9). In the P. oryzihabitans strain AXSa06 genome, there are 4929 protein coding sequences (9). Besides AXSa06 and AXSa07 strains, the genome of GCMCC 6169, a soil strain of P. oryzihabitans, was shown to encode the protein POEP1 which aids cellulose breakdown. Although POEP1 does not break down cellulose directly, it synergistically enhances the activity of cellulase, a protein that directly hydrolyzes cellulose for energy (10).

4. Cell structure

Interesting features of cell structure. Can be combined with “metabolic processes”

5. Metabolic processes

Describe important sources of energy, electrons, and carbon (i.e. trophy) for the organism/organisms you are focusing on, as well as important molecules it/they synthesize(s).

6. Ecology

Habitat; symbiosis; contributions to the environment.

7. Pathology

How does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.

8. Current Research

Include information about how this microbe (or related microbes) are currently being studied and for what purpose

9. References

It is required that you add at least five primary research articles (in same format as the sample reference below) that corresponds to the info that you added to this page. [Sample reference] Faller, A., and Schleifer, K. "Modified Oxidase and Benzidine Tests for Separation of Staphylococci from Micrococci". Journal of Clinical Microbiology. 1981. Volume 13. p. 1031-1035.