Psychrobacter luti

Classification

Genus: Psychrobacter

Domain; Phylum; Class; Order; family [Others may be used. Use NCBI link to find]

Species

NCBI: Taxonomy

Genus species

Description and Significance

Psychrobacter was created by Juni & Heym (1986) to accommodate a group of non-motile, oxidase-positive, non-pigmented, chiefly psychrotolerant, Gram-negative rods or coccobacilli isolated from the skin of fish and chickens and from various processed foods.

Psychrobacter organisms thrive in cold environments which constitute an ecological niche. A source of Psychrobacter organisms is Anarctic environments.

The type strain, strain NF11T (=LMG 21276T=CECT 5885T), was isolated from muddy soil collected from the inlet Admiralty Bay on King George Island, South Shetland Islands, Antarctica.

Describe the appearance, habitat, etc. of the organism, and why you think it is important.

Genome Structure

Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Psychrobacter luti strain LMG 21276 DNA gyrase subunit B(gyrB)gene, partial cds. 2238 bp of DNA in a linear structure isolated from Siberian permafrost.(Bakermans,C et al 2005).

Psychrobacter luti strain NF11 16S ribosomal RNA, partial sequence. 1491 bp of rRNA in a linear structure. (Bozal,N 2008)

Cell Structure, Metabolism and Life Cycle

The isolates were oxidase-positive, halotolerant, Gram-negative,considerable halotolerance, non-motile coccobacilli with a strictly oxidative metabolism.

Neither diffusible pigments nor bioluminescence were observed. The cells of all bacterial isolates presented capsules and were about 0.4–1.8 by 0.4–0.8 mm in size.

The strains were moderately halophilic and tolerated NaCl levels of about 9.5–12.5%.

whereas colonies of NF11T, NF18, NF19 and NF20 were smooth, opaque, non-circular and spread little throughout the growth medium, with similar dimensions.

The pH range for growth was 6–9.5 and the growth temperature range was 4–30 oC for all the strains isolated

All strains were oxidase- and catalase-positive and capable of oxidative metabolism.

Except for strain NF11T, all the strains possessed urease activity. Does not

Strains NF11T, NF18, NF19 and NF20 reduced nitrate to nitrite.

deaminated phenylalanine

-None of the strains was capable of deaminating tryptophan.

-sensitive to penicillin.

-possessed L-alanine aminopeptidase, again indicating their Gramnegative character.

Able to grow in the absence of NaCl and

-can tolerate 9.5% (w/v) NaCl

Ecology and Pathogenesis

Psychrobacter is found in Anarctic environments which contributes towards its ecologically niche. Due to this environment, Psychrobacter has been observed in the GI tract of Atlantic Cod.

References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

National Center for Biotechnology Information, U.S. National Library of Medicine 8600 Rockville Pike, Bethesda MD, 20894 USA

Author

Page authored by Hahyung Y. Kim and Benjamin Kamphuis, student of Prof. Jay Lennon at Michigan State University.

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