Psychrobacter luti

From MicrobeWiki, the student-edited microbiology resource

Classification

Genus: Psychrobacter

Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;Moraxellaceae; Psychrobacter.

Species

NCBI: Taxonomy

Psychrobacter luti

Description and Significance

Within the muddy soil of the inlet Admiralty Bay on King George Island, South Shetland Islands, Antarctica the species Psychrobacter luti was found and isolated. This non-motile, halotolerant, psychrotolerant bacteria is apart of a unique ecological ecosystem in which studies focusing on singling out which metabolic pathways microbes go through, and understanding the evolution of microbes in many lakes. At one point or another, most of the lakes of the Earth were covered by glaciers. In many cases, the glaciers would keep the lakes sealed. Interestingly, Antarctica holds some key lakes in which are still ice sealed. Through studying these lakes and their microbes, we can better understand the evolution of microbe metabolism over time.

Genome Structure

Psychrobacter luti strain LMG 21276 DNA gyrase subunit B(gyrB)gene, partial cds. 2238 bp of DNA in a linear structure isolated from Siberian permafrost (Bakermans,C et al 2005). The protein DNA gyrase subunit B's gene was sequenced and analyzed also and revealed different gene regions within its sequence which coded for other functions. Such regions as a histidine kinase site and a topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain which removes both positive and negative supercoils of DNA. Also, Psychrobacter luti strain NF11 16S ribosomal RNA was partially sequenced. It revealed 1491 bp of rRNA in a linear structur (Bozal,N 2008). It has recently been found that the genome of Psychrobacter luti is 95% similar to other microbes found in sea sponges of Southern China, which only function in the colder times of the year. Also, the other microbes which were also compared to the genome of the sea sponge microbes were just as similar such as microbes apart of the genus Sporosarcina, the genus Shewanella, and even the genus Bacillus. This forms the conclusion that the genome of Psychrobacter luti is the same structure as the genome of microbes surviving optimally in colder temperatures.

Cell Structure, Metabolism and Life Cycle

The isolates were oxidase-positive, halotolerant, Gram-negative,considerable halotolerance, non-motile coccobacilli with a strictly oxidative metabolism.

Neither diffusible pigments nor bioluminescence were observed. The cells of all bacterial isolates presented capsules and were about 0.4–1.8 by 0.4–0.8 mm in size.

The strains were moderately halophilic and tolerated NaCl levels of about 9.5–12.5%.

whereas colonies of NF11T, NF18, NF19 and NF20 were smooth, opaque, non-circular and spread little throughout the growth medium, with similar dimensions.

The pH range for growth was 6–9.5 and the growth temperature range was 4–30 oC for all the strains isolated

All strains were oxidase- and catalase-positive and capable of oxidative metabolism.

Except for strain NF11T, all the strains possessed urease activity. Does not

Strains NF11T, NF18, NF19 and NF20 reduced nitrate to nitrite.

deaminated phenylalanine

-None of the strains was capable of deaminating tryptophan.

-sensitive to penicillin.

-possessed L-alanine aminopeptidase, again indicating their Gramnegative character.

Able to grow in the absence of NaCl and

-can tolerate 9.5% (w/v) NaCl

Ecology and Pathogenesis

Psychrobacter luti thrives in the cold extremes of the biosphere. Cold (psychrotolerant) and salty (halotolerant) environments are its ecological niche with places such as Antarctic glacier mud serve as a prime environment for its livelihood. Many scientist infer that these subzero temperatures of Antarctica create environment where very little to no microbial activity is observed. This is not true when such microbes as Psychrobacter luti were discovered as being microbes that not only survive in these environments, but maintain a consisted, often growing microbial biomass. However, the environment Psychrobacter relatives are found in are not always extremes such as Antarctica.

Another Psychrobacter found in very similar environmets is Psychrobacter glacincola. According to a study done by Ringo et al., Psychrobacter glacincola is also found in the gastrointestinal tract of Atlantic cod fed soybean meal. According to another study done by Li et al, both Psychrobacter luti and Psychrobacter glacincola are in the upper 90% in having a similar genome to the microbes found in South China sea sponges (only in the colder times of the year).

Psychrobacter luti is one of the prime reducers of nitrate to nitrite. Also, with the use of oxidase and catalase in its striclty aerobic metabolism, it provides oxygen, water, and hydrogen peroxide to other microbes. Since there are these microbial biomasses present in the glacier mud of Antarctica, the biomass often prevents freezing at all depths of the mud, just as the lakes of Antarctica, but also a cohesive attribute which can hold glacier mud bound.

Even though many of these microbes tend to form biomass, the amount of nutrients is often scarce in an extreme environment. However, they can feed off of each other's metabolic products and do not have to worry about predation from other organisms.

One attribute of these cold environments is the lack of pathogens. Because of this scarcity in pathogens, the pathogenesis of Psychrobacter luti is not applicable.

References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

National Center for Biotechnology Information, U.S. National Library of Medicine.

Author

Page authored by Hahyung Y. Kim and Benjamin Kamphuis, student of Prof. Jay Lennon at Michigan State University.

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