Pyrolobus fumarii: Difference between revisions

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==Classification==
==Classification==

Revision as of 00:20, 16 April 2008

A Microbial Biorealm page on the genus Pyrolobus fumarii

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Classification

Higher order taxa:

Archaea; Crenarchaeota; Thermoprotei; Desulfurococcales; Pyrodictiaceae

NCBI: Taxonomy Genome

Species:

Pyrolobus fumarii (Synonyms: Pyrolobus Blochl et al. 1999)

Description and Significance

Pyrolobus fumarii are made up of cells that are regulary to irregulary lobed cocci. Chemolithoautotrophic growth by anaerobic and microaerophilic H2 oxidation with NO3-, S2O32-, and O2 as electron accepters. By doing so well at temperatures of 95ºC to 113ºC, our knowledge about the upper limits of life is expanded.

Genome Structure

DNA Base composition Determined by a direct analysis of its mononucleosides and melting point analysis,Pyrolobus fumarii has a cytosine-guanine composition of 52.9mol% and 53.4mol% respectively. (Blöchl et al. 1997)

DNA-DNA homology The closest relatives of Pyrolobus fumarii are members of Pyrodictium based off 16S rRNA partial sequencing. There is insignificant DNA homology between Pyrolobus fumarii, Pyrodictium occultum, and Pyrodictium abyssi as shown in a DNA/DNA hybridization. (Blöchl et al. 1997)

Cell Structure

Pyrolobus fumarii are cells are regularly to irregularly lobe-shaped cocci (0.7-2.5 microm in diameter) arranged singly. A protein surface layer composes the cell envelope, which exhibits p4 symmetry.

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Metabolism

Pyrolobus fumarii is a facultatively aerobic obligate chemolithoautotroph. Moreover, P. fumarii is hydrogen – dependent gaining energy by H2 oxidation at a very low oxygen concentration and uses CO2¬ as the single carbon source.

There are three different metabolic types observed on electron acceptor dependence:

  1. Nitrate Ammonification
    1. Nitrate is used as a terminal acceptor during strict anaerobic conditions wherein NO3- and H2 are present. In the process, ammonia is formed during NO3- reduction.
4H2 + H+ + NO3- --> NH4 + OH- + 2H2O
  1. Thiosulfate reduction
    1. Formation of H2S in the presence of H2 and uses S2O32- as electron acceptor also during anaerobic conditions.
  2. Microaerophilic hydrogen oxidation
    1. Under increased O2 condition, H2 and O2 were the only contributing elements for growth. In this reaction, O2 serves as the electron acceptor.

In addition, acetate, pyruvate, glucose, starch, or elemental sulfur are capable of inhibiting the growth of P. fumarii and these organic compounds do not contribute in growth stimulation.

P. fumarii isolates elicited hydrogenase activity. It used methylene blue as the artificial electron acceptor and the process was membrane –associated.

To allow existence in aggressive conditions, heat shock proteins were also detected in P. fumarii. Isolates were observed to have a chaperone-like heat shock protein.

Recent study (Gonc¸alves et. al.) shows that the major organic solute composition of P. fumarii is DIP or Di-myo-inositol phosphate. The accumulation of DIP is considered to be one of the metabolic features of P. fumarii enabling it to survive as hyperthermophile living at the upper temperature and pressure limits for life.

Metabolic features of Pyrolobus suggest its capability to exist in a pre-Earth condition with solely volcanic activity and liquid water conditions.

Ecology

Pyrolobus fumarii is an extreme hyperthermophile isolated in the wall of a black smoker, or a deep-sea hydrothermal vent formed by sulfur-bearing minerals from beneath the Earth's crust, located in the Mid-Atlantic Ridge. It thrives at temperature of superheated water with optimal temperature at 106ºC and will not grow below 90ºC. Its optimum pH is 5.5 and grew between 4.0 – 6.5 while salt concentration is between 1%-4% with 1.7% as its optimum.

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References

Blöchl, E., R. Rachel, S. Burggraf, D. Hafenbradl, H.W. Jannasch, and K.O. Stetter. 1997. Pyrolobus fumarii, gen. and sp. nov., represents a novel group of archaea, extending the upper temperature limit for life to 113ºC. Exptremeophiles 1:14-27.

Goncalves, L.G., P. Lamosa, R. Huber, H. Santos. 2008. Di-myo-inositol phosphate and novel UDP-sugars accumulate in the extreme hyperthermophile Pyrolobus fumarii. Extremophiles.

Huber, H., R. Huber, and K.O. Stetter. 2006. Thermoproteales. Prokaryotes 3:10-22.