Rhodanobacter denitrificans: Difference between revisions
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=Description= | =Description= | ||
''Rhodanobacter denitrificans'' is a yellow-pigmented, gram-negative, non-sporulating, motile,slow-growing, and facultatively anaerobic rod-shaped bacterium with the ability to perform complete denitrification [8]. For ''Rhodanobacter'' species, denitrification was not a characteristic until the discovery of ''R. denitrificans'' [6]. Denitrification is the conversion of nitrate to N2 through nitrite, nitric and nitrous oxide intermediates catalyzed by microbial enzymes [11]. Only two species of ''Rhodanobacter'', one of them being ''Rhodanobacter denitrificans'', is capable of this process [8]. In the absence of oxygen, growth is fueled by the use of nitrate, nitrite, and nitrous oxide as electron acceptors [8]. ''Rhodanobacter denitrificans'' has a salt tolerance up to 2% NaCl [8]. Growth was observed at pH 4-8 and 10-35ºC, optimally at pH 6.5 and 30ºC [8]. The two strains of ''R. denitrificans'', 2APBS1 and 116-2, were found at Oak Ridge Integrated Field Research Challenge (ORIFRC) [8]. ORIFRC is a model nuclear legacy site with high levels of nitrate and acidity, with uranium and other heavy metal contaminants [8]. At ORIFRC, 2APBS1 was isolated from Area 2 (pH 6-7 and nitrate level <2 mM) and 116-2 was isolated from Area 3 (pH 3-4 and nitrate level 10s to 100s mM) [8]. The two strains are highly abundant and active in acidic, nitrate rich subsurface environments with high metal (e.g. uranium) concentrations [8]. The characteristic of survival in these harsh environments allow ''R. denitrificans'' to conduct bioremediation of the contamination sites by denitrification [8]. | ''Rhodanobacter denitrificans'' is a yellow-pigmented, gram-negative, non-sporulating, motile,slow-growing, and [http://en.wikipedia.org/wiki/Facultative_anaerobic_organism facultatively anaerobic] rod-shaped bacterium with the ability to perform complete [http://en.wikipedia.org/wiki/Denitrification denitrification] [[#References|[8]]]. For ''Rhodanobacter'' species, denitrification was not a characteristic until the discovery of ''R. denitrificans'' [[#References|[6]]]. Denitrification is the conversion of nitrate to N2 through nitrite, nitric and nitrous oxide intermediates catalyzed by microbial enzymes [[#References|[11]]]. Only two species of ''Rhodanobacter'', one of them being ''Rhodanobacter denitrificans'', is capable of this process [[#References|[8]]]. In the absence of oxygen, growth is fueled by the use of nitrate, nitrite, and nitrous oxide as electron acceptors [[#References|[8]]]. ''Rhodanobacter denitrificans'' has a salt tolerance up to 2% NaCl [[#References|[8]]]. Growth was observed at pH 4-8 and 10-35ºC, optimally at pH 6.5 and 30ºC [[#References|[8]]]. The two strains of ''R. denitrificans'', 2APBS1 and 116-2, were found at [http://www.esd.ornl.gov/orifrc/ Oak Ridge Integrated Field Research Challenge (ORIFRC)] [[#References|[8]]]. ORIFRC is a model nuclear legacy site with high levels of nitrate and acidity, with uranium and other heavy metal contaminants [[#References|[8]]]. At ORIFRC, 2APBS1 was isolated from Area 2 (pH 6-7 and nitrate level <2 mM) and 116-2 was isolated from Area 3 (pH 3-4 and nitrate level 10s to 100s mM) [[#References|[8]]]. The two strains are highly abundant and active in acidic, nitrate rich subsurface environments with high metal (e.g. uranium) concentrations [[#References|[8]]]. The characteristic of survival in these harsh environments allow ''R. denitrificans'' to conduct bioremediation of the contamination sites by denitrification [[#References|[8]]]. | ||
Revision as of 08:27, 14 December 2012
IN PROGRESS
Classification
Taxa
Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Rhodanobacter; denitrificans [8].
Strains
2APBS1 and 116-2 [8].
Description
Rhodanobacter denitrificans is a yellow-pigmented, gram-negative, non-sporulating, motile,slow-growing, and facultatively anaerobic rod-shaped bacterium with the ability to perform complete denitrification [8]. For Rhodanobacter species, denitrification was not a characteristic until the discovery of R. denitrificans [6]. Denitrification is the conversion of nitrate to N2 through nitrite, nitric and nitrous oxide intermediates catalyzed by microbial enzymes [11]. Only two species of Rhodanobacter, one of them being Rhodanobacter denitrificans, is capable of this process [8]. In the absence of oxygen, growth is fueled by the use of nitrate, nitrite, and nitrous oxide as electron acceptors [8]. Rhodanobacter denitrificans has a salt tolerance up to 2% NaCl [8]. Growth was observed at pH 4-8 and 10-35ºC, optimally at pH 6.5 and 30ºC [8]. The two strains of R. denitrificans, 2APBS1 and 116-2, were found at Oak Ridge Integrated Field Research Challenge (ORIFRC) [8]. ORIFRC is a model nuclear legacy site with high levels of nitrate and acidity, with uranium and other heavy metal contaminants [8]. At ORIFRC, 2APBS1 was isolated from Area 2 (pH 6-7 and nitrate level <2 mM) and 116-2 was isolated from Area 3 (pH 3-4 and nitrate level 10s to 100s mM) [8]. The two strains are highly abundant and active in acidic, nitrate rich subsurface environments with high metal (e.g. uranium) concentrations [8]. The characteristic of survival in these harsh environments allow R. denitrificans to conduct bioremediation of the contamination sites by denitrification [8].
