Salegentibacter mishustinae

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Classification

Higher order taxa

Bacteria; Phylum; Class; Order; Family; Genus: Bacteria; Bacteroidetes; Flavobacteria; Flavobacteriales; Flavobacteriaceae; Salegentibacter


Species

Genus species: Salegentibacter mishustinae

Description and significance

This species is from a microbial community that is associated with Strongylocentrotus intermedius which is a sea urchin found in Troista Bay, Gulf of Peter the Great, Sea of Japan. It was discovered in September 2002 in a water depth of 3 meters. This species is an asporogenic rod shaped gram-negative bacteria. It is strictly aerobic, chemo-organotropic and non-motile. The cells are about 0.5-0.7 µm wide and 2.5-5.1 µm long. Colonies of this species appear to form circular, shiny and convex, 1-3 mm in diameter on marine agar. They produce a yellow non-diffusible pigment.

Growth Conditions

No growth of this species occurs without the presence of Na+. The concentration of Na+ that allows for the growth of this species varies between 1 to 18 % NaCl. This bacterium can grow in temperatures varying from 4 to 36˚C.

Cell Metabolism

After conducting some tests and the results show that it is able to hydrolyze gelatin, casein, elastin, alginic acids, starch and Tweens 20, 40 and 80. It can form acid from D-maltose, L-raffinose and D-sucrose. This bacteria is able to produce H2S. There were also some quantities of fatty acids found like fatty acids are i15 : 1 (12.3 %), i15 : 0 (7.5 %), a15 : 0 (7.9 %), 15 : 0 (6.7 %), i17 : 0 3-OH (8.6%) and summed feature 3 (7.9 %), comprising 16 : 1v7 and i15 : 0 2-OH fatty acids.

Phylogenetic Affinities

Many species from the genus Salegentibacter were analyzed and compared to Salegentibacter mishustinae to see the phylogenetic affinities. Salegentibacter mishustinae was determined to be closely related to the species Salegentibacter salegens, which was isolated from a hypersaline lake in Antarctica and Salegentibacter holothuriorum, which was recovered from the edible holothurian Apostichopus japonicus. The phylogenetic affinities were determined by genomic DNA extraction, PCR and 16S rRNA gene sequencing.

Antibiotic Susceptibility and Resistance

Based of studies, this species is found to be susceptible to ampicillin, oleandomycin, benzylpenicillin, lincomycin and tetracycline. It is also discovered to have a resistances to the following; carbenicillin, kanamycin, neomycin, streptomycin, gentamicin and polymyxin B.

References

Bernardet, J.F. "Taxa Covered by the ICSP Subcommittee on Taxonomy of Flavobacterium- and Cytophaga-like Bacteria." Taxa Covered by the ICSP Subcommittee on Taxonomy of Flavobacterium- and Cytophaga-like Bacteria. N.p., 28 Feb. 2011. Web. 12 Mar. 2014.

Nedashkovskaya, O. I. "Salegentibacter Mishustinae Sp. Nov., Isolated from the Sea Urchin Strongylocentrotus Intermedius." International Journal Of Systematic And Evolutionary Microbiology 55.1 (2005): 235-38. Print.

Kroh, Andreas. "Strongylocentrotus Intermedius." World Enchinoidea Database. N.p., 07 Sept. 2010. Web. 12 Mar. 2014. <http://www.marinespecies.org/echinoidea/aphia.php?p=taxdetails&id=513570>.


Edited by Erin Wardwell, student of Rachel Larsen at the University of Southern Maine