Serena and Beth - Bacillus thurigiensis

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Classification

[1] 

Domain: Bacteria

Phylum: Firmicutes

Class: Bacilli

Order: Bacillales

Family :Bacillaceae

Species

NCBI: Taxonomy

Bacillus

Habitat Information

This organism Bacillus thuringiensis was collected and isolated into a sample of 3 tablespoons of the dampen soil. It was gathered at the soil ground from a homeowner's front lawn on a street named Rei Tang Loop in Kyle, Texas, at the location Latitude: 30.0031643 and Longitude: -97.8756188 and Sea level: 221 m.

The collection laid within a sloped old garden patch that was within a stoned surrounding near a cactus plant and near a home dwelling.

The soil sample was collected on January 28th, 2015.
The air temperature was 73 fahrenheit with humidity at 24 percent, with no rainfall prior to 24 hours, with solar radiation being at 2 of 10 UV.
Soil was collected from the surface to the depth of 1 inch. The location was collected around 3:30 PM in a semi-shaded semi-sunny location with no foot traffic.

Description and Significance

When isolated and grown in an LB agar plate, Bacillus thuringiensis colonies appeared irregular in shape, slightly umbonate, with smooth entire margins. Under the microscope the Bacillus thuringiensis after performing a Gram stain test results in being a Gram-positive organism, and is rod-shaped in nature and is approximately 1 µm in width and 5 µm in length. When a match patch test was done with 11 other soil samples, Bacillus thuringiensis showed clear evidence of clearing around the colony when grown on an LB plate that contained Staphylococcus aureus and / or Escherichia coli, Including of course the Bacillus thuringiensis itself. This is reason as to why this bacteria was hand pick to focus our studies on since in both scenarios, it suggested possible antimicrobial activity, despite it presenting it's own growth within another bacteria.

Genome Structure

Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.

Foward: GACGGAGCAACGCCGCGTGAGTGATGAAGGCTT TCGNGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACCTAACCAGAA AGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGC GCGCAGGTGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGAGACTTGAGTG CAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGAGGAACACCAGTGGCGAAGGCGACTTT CTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACG ATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGAAGTTAACGCATTAAGCACTCCGCCTGGGGAGTACGGCC GCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGA ACCTTACCAGGTCTTGACATCCTCTGACAACCCTAGAGATAGGGCTTCTCCTTCGGGAGCAGAGTGACAGGTGGTGCATG GTTGTCGTCAGCTCGTGNCGTGAGATGTCATA

Reverse: ACCACCTGTCACTCTGCTCCCGAAGGAGAAGCCCTATCTCTAGGGTTGTCAGAGGATGTCAAGACCTGG TAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAG CCTTGCGGCCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAACTTCAGCACTAAAGGGCGGAAACCCTCTAACACTTAGC ACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACA GACCAGAAAGTCGCCTTCGCCACTGGTGTTCCTCCATATCTCTACGCATTTCACCGCTACACATGGAATTCCACTTTCCT CTTCTGCACTCAAGTCTCCCAGTTTCCAATGACCCTCCACGGTTGAGCCGTGGGCTTTCACATCAGACTTAAGAAACCAC CTGCGCGCGCTTTACGCCCAATAATTCCGGATAACGCTTGCCACCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCC GTGGCTTTCTGGTTAGGTACCGTCAAGGTGCCAGCTTATTCAACTAGCACTTGTTCTTCCCTAACAACAGAGTTTTACGA CCCGAAAGCCTTCATCACTCACGCGGCGTTGCTCCGTCAGACTTTCGTCCATTGCGGAAGATTCCCTACTGCTGCCTCCC

Cell Structure, Metabolism and Life Cycle

Interesting features of cell structure; how it gains energy; what important molecules it produces.

Physiology and Pathogenesis

Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.

References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

Author

Page authored by Serena Perez and Elizabeth Koch, student of Prof. Kristine Hollingsworth at Austin Community College.