Serratia liquefaciens

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A Microbial Biorealm page on the genus Serratia liquefaciens

Classification

Higher order taxa [1]

Domain: Bacteria

Phylum: Proteobacteria

Class: Gammaproteobacteria

Order: Enterobacteriales

Family: Enterbacteriaceae

Genus: Serratia

Species

NCBI: Taxonomy

Serratia liquefaciens

Description and significance

S. liquefaciens is a straight rod shaped bacterium with a diameter of 0.5-0.8 μm, a length of 0.9-2.0 μm, and Gram negative. Serratia species are usually motile and contain peritrichous flagella. [Bergey’s Manual of Determinative Bacteriology:9th edition p.187]

S. liquefaciens are facultative anaerobes making oxygen inessential to survival. They can inhabit both aerobic and anaerobic environments. Thus, S. liquefaciens is a widespread bacteria found in the environment and capable of colonizing on soil, water, plants, and the digestive tracts of rodents, insects, fish, and humans.[2] However, it must be noted that S. liquefaciens is not a normal component of human fecal flora. [3]

Not much is actually known about Serratia liquefaciens. In fact, up until 1971, it was actually characterized as Enterobacter liquefaciens. After extensive comparison with S. marcescens, E. liquefaciens was determined to be closely related to S. marcescens and the genus was changed to Serratia.[4]

Following the genus change, little can be found regarding S. liquefaciens. However, it has been proven to have certain antifungal properties in a plant’s rhizosphere. [5] It is also a pathogenic bacterium that has been associated with severe nosocomial infections.

Genome structure

Limited information can be found on the genome of Serratia liquefaciens. The entire genome has not been sequenced so the complete size and contents of the genome are unknown along with number of chromosomes. Several partial sequences of 16S ribosomal RNA have been analyzed and are indicated as linear strands.[6] [7] [8] However, the close relative S. marcescens has a single circular chromosome that is signature to many bacterial species. [9] It has also been proven that some bacteria may have multiple circular chromosomes or linear chromosomes and linear plasmids.[10]

Cell structure and metabolism

Serratia liquefaciens has a straight rod structure that is Gram negative. Gram negative organisms have a thinner layer of peptidoglycan surrounded by an inner and outer membrane. Serratia species are usually motile and have peritrichous flagella.

S. liquefaciens is able to ferment glucose and other carbohydrates (i.e. sucrose, D-mannose,D- mannitol, maltose, salicin, and trehalose) as a source of carbon while producing acid as a byproduct. S. liquefaciens also utilizes lysine, ornithine, and hydrolyzes gelatin, as a source of carbon and energy for growth. It can also hydrolyze DNA and reduce nitrate to nitrite –which may be a byproduct of anaerobic respiration and.[Bergey’s 217]

Ecology

S. liquefaciens is commonly found on many plant species and specifically in the rhizosphere. A rhizosphere is a 1mm area of soil that surrounds the root of a plant and is teeming with biological and chemical activity. The biological makeup is influenced by the specific root where a symbiotic relationship forms. The root releases water soluble compounds into the rhizosphere that provides the microorganisms with nutrients. [11] Meanwhile, the microorganisms will benefit the plant in a variety of ways. Serratia species – including S. liquefaciens - are known to have antifungal properties that benefit oilseed rape. The antifungal properties of the genus produce antibiotics, siderophores, and lytic enzymes like chitinase. Furthermore, S. liquefaciens is specifically linked to protection carnation rhizospheres from root cutting.[12]

Pathology

The Serratia genus as a whole is responsible for about 2% of nosocomial infections; most of which are attributed to S. marcescens. They tend to colonize in respiratory and urinary tracts of adults.[13] In 1950, the genus Serratia was thought to be harmless. A study by the United States Army wished to determine the effects that biological warfare may have on the United States, about 117 square miles of the San Francisco area was sprayed with an aerosol of S. marcescens. After approximately 800,000 people were exposed, a resulting epidemic of serratia-related pneumonia hit the area killing many people. Although the intentions of the Army were not to cause harm the the population, the effects of Serratia were then discovered. [14]

Since the tragedy of 1950, more exploration into the pathogenic effects of Serratia have been documented. There are many different symptoms and infections that may arise from S. liquefaciens exposure. While some strains are non-pathogenic, others cause urinary tract infections, bloodstream infections, sepsis, pneumonia, meningocephalitis, and other debilitating effects or even death.[15] It is relatively rare for S. liquefaciens outbreaks to occur, and when they do, they are often associated with nosocomial infections due to poor hygiene. In a three month span during 1999, eight patients at a hospital were diagnosed with S. liquefaciens infections. After investigation took place, the infection was attributed to non-sterile equipment used to calibrate machinery. Investigations also yielded improper hand washing, and multiple uses of heparin and insulin vials. The infections were successfully treated with antibiotics. [16]

While many other investigations of poor hygiene has resulted from S. liquefaciens infections, they are not all as easily treated. In an investigation following infection after a Vitamin C infusion research indicated poor hygiene as a cause for the nosocomial infection. After receiving the transfusion the patient complained of neck pain, fever, and vomiting. However, overnight she was taken to the emergency room with septic shock, meningism, and loss of consciousness. Once admitted to the ICU, the patient was comatose with weakened pulse. After further complications arose, the patient was finally dismissed from the hosptital after 68 days of treatment to continue therapy at a rehabilitation center. The fatality rate is about 75% for S. liquefaciens sepsis.[17]

Current Research and or Application to Biotechnology

Now that the pathogenic effects of the Serratia species are becoming more widely known, researchers are turning their attention to the antibiotic resistance of S. liquefaciens. 41 strains of the S. liquefaciens complex have been identified and their susceptibility to 70 different antibiotics was recorded. While all species in the Serratia genus were naturally resistant to at least fifteen different antibiotic strains. This information is very helpful when treating bacterial infections. Studies also mapped the antibiotics that the species is most susceptible to as well. This had never been done prior to the last decade even though the danger of Serratia has been known since the 1950’s. It has also been discovered that all of the species of S. liquefaciens complex are actually involved in human infections. [18]

Research is also being done to determine exactly how Serratia liquefaciens infections have been spreading – particularly in the sense of epidemics. While it is known that a wide variety of clinical manifestations have occurred over the past couple of years, the particular sources of infection are difficult to identify. Main transmission modes are contamination of hospital staff hands with patients acting as reservoirs for infection. However, since there are so many resistant strains of S. liquefaciens it is important to know exactly how the infections are spread in order to control nosocomial epidemics. It is already known that infections usually harbor in the respiratory tract. However, it was confirmed that the majority of patients had lung colonization. This is not the only mode of transmission. The bacterial presence has also been confirmed in blood cultures. The most frequent mode of transmission is via throat and fecal carriage. However, nasal and urinary carriage is prevalent in 31% and 22% of the cases examined. Fecal carriage is also associated more with pediatric patients than adults. The confirmation that patients are reservoirs for infection increased the importance of hand washing techniques since hands are a likely mode of transmission. Due to this information, it can be difficult to eradicate a S. liquefaciens infection. This stresses the importance of planning infection control programs and researching the best ways to limit the passage of infection while carefully monitoring antibiotic dispersal due to the high resistance found in some strains.[19]

References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

Edited by student of Dr. Lynn M Bedard, DePauw University http://www.depauw.edu