Fungi; Basidiomycota; Agaricomycotina; Agaricomycetes; Polyporales
Description and Significance
"Sparassis crispa" is a species in the Fungi kingdom found all over the world in northern temperate forests (1). They are brown rot fungi which grow at the base of conifer trees such as pine, spruce, cedar, and larch (2). The fruiting body of the fungus is known as cauliflower fungus because of the resemblance it has with the vegetable, with flat and curly lobes that come in cream, white, and yellow. Cauliflower fungus are an edible mushroom, known for their culinary and medicinal benefits and are prized throughout Europe and Asia for their mild flavor and multiple health benefits such as anti-tumor and anti-cancer (1). "Sparassis crispa" belong to the genus "Sparassis" which include at least 8 different clades which mostly differ in morphological features but also can differ in cellular features such as the presence or absence of clamp connections (3).
Life Cycle, Cell Structure, Metabolism
"Sparassis crispa (S. crispa)" are members of the phylum Basidiomycota. The species exhibits bifactorial heterothallism (4), requiring two compatible partners to produce sexual spores and mating alleles with two loci. The basidiocarp consists of branched flabellae with spore baring hymenium on both sides (5). S. crispa contains a monomitic hyphal system and is a clamp connection producing species (3). Basidiospores are hyaline, smooth, subglobase with thin walls (5). Basidia contain 2-4 sterigmata and basidiospore are generally larger with a low number of spores produced on a single basidium (5).
"S. crispa" contains 32 Contigs, a 39.0-Mb genome, 51.4% GC content, 13,157 protein-coding genes, 1,669.3 bp average genome length, 1,326.1/1,044 bp average protein-coding sequence size, a 5.7 average exon number, a 233.6/137 bp average exon size and a 73.4/55 bp average intron size (1). Phylogenetically, "S. crispa" is most closely related to "Postia placenta", a brown rot fungus and both of their divergence is estimated to be around 94 million years ago (1).
Ecology and Pathogenesis
"S. crispa" is a brown rot fungi which can grow as parasites or saprobes on the roots of coniferous trees. They can be found at the base of living or decaying hardwood trees. "S. crispa" can enter through scars of roots and stems of conifers (6) and live on a living or dead host.
[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.
https://www.sciencedirect.com/science/article/pii/S2221169115001069 Lee, D.J., Jang M.C., Jo A R., Choi, H.J., Kim, K., Chi, Y. "Noble strain of Sparassis latifolia produces high content of β-Glucan". Asian Pacific Journal of Tropical Biomedicine.2015. Volume 5, Issue 8. p. 629-635.
http://www2.clarku.edu/faculty/dhibbett/mbinder/pdf/Wang%20Sparassis%202004.pdf Wang, Z., Binder, M., Dai, Y., Hibbet, D. "Phylogenetic relationships of Sparassis inferred from nuclear and mitochondrial ribosomal DNA and RNA polymerase sequences". Mycologia. 2004. 96(5). p. 1015-1029.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4004946/ Park, H., Ka, K., Ryu, S. "Enhancement of β-Glucan Content in the Cultivation of Cauliflower Mushroom (Sparassis latifolia by Elicitation". Mycobiology. 2014. 42(1). p. 41-45.
https://www.nature.com/articles/s41598-018-34415-6#Tab1 Kiyama, R., Furutani, Y., Kawaguchi, K., Nakanishi, T. "Genome sequence of the cauliflower mushroom Sparassis crispa (Hanabiratake) and its association with beneficial usage". Scientific Reports. 2018. 8. Article number 16053.
http://agris.fao.org/agris-search/search.do;jsessionid=94BA80B17CEEABC6AA573692CFBBF65B?request_locale=ru&recordID=KR2010003826&query=&sourceQuery=&sortField=&sortOrder=&agrovocString=&advQuery=¢erString=&enableField= Park, H., Oh, D.S., Ka, K.H., Ryu, S.R. "Environmental Condition for the Butt-Rot of Conifers by Cauliflower Mushroom (Sparassis crispa) and Wood Quality of Larix kaempferi Damaged by the Fungus". Korea Agricultural Science Digital Library. 2009.
Page authored by Alex Atkin, student of Dr. Marc Orbach, University of Arizona .