Streptomyces Unknown Family: Difference between revisions

From MicrobeWiki, the student-edited microbiology resource
Line 28: Line 28:


==Physiology and Pathogenesis==
==Physiology and Pathogenesis==
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).<br>
<b>BIOCHEMICAL TEST RESULTS</b>
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br>
*<b>Gram Reaction:</b> positive
*<b>Capsule Stain:</b> negative
*<b>Endospore Stain:</b> negative
*<b>Motility:</b> positive
*<b>Phenol Red Broth Tests:</b> <i>Glucose:</i> yellowish-pink, negative; <i>Lactose:</i> red-pink, negative; <i>Sucrose:</i> red-pink, negative
*<b>Starch Hydrolysis Test:</b> negative
*<b>Casein Hydrolysis Test:</b> positive
*<b>Gelatin Hydrolysis Test:</b> negative
*<b>DNA Hydrolysis Test:</b> negative
*<b>Lipid Hydrolysis Test:</b> negative
*<b>Methyl Red Test:</b> negative
*<b>Voges Proskauer Test:</b> negative
*<b>Citrate Test:</b> positive
*<b>SIM Tests:</b> negative for all
*<b>Nitrate Reduction:</b> negative
*<b>Urea Hydrolysis:</b> positive
*<b>Triple Sugar Iron Agar:</b> negative for all
*<b>Oxidase Test:</b> negative
*<b>Eosin Methylene Blue Agar (EMB) Test:</b> positive
*<b>Hektoen Enteric Agar (HE) Test:</b> green, negative, no growth
*<b>MacConkey Agar Test:</b> negative
*<b>Decarboxylation Tests:</b> <i>Arginine:</i> no change; <i>Lysine:</i> negative; <i>Orinithine:</i> negative
*<b> Phenylalanine Deaminase Test:</b> negative
*<b> Catalase Test:</b> negative
*<b> Blood Agar Test:</b> negative
*<b>Mannitol Salt Agar (MSA) Test:</b> negative
*<b>Phenylethyl Alcohol Agar (PEA) Test:</b> negative
*<b>Bacitracin/Optochin Susceptibility Test:</b> <i>Bacitracin:</i> non-resistant; <i>Optochin:</i> non-resistant
*<b>Bile Esculin Test: </b> negative
*<b>6.5% Salt Tolerance Test: </b>slight positive


==References==
==References==

Revision as of 05:26, 14 April 2018

This student page has not been curated.

Classification

Domain; Phylum; Class; Order; family [Others may be used. Use NCBI link to find]

Species

NCBI: Taxonomy

Genus species

Habitat Information

Describe the location and conditions under which the organism was isolated.

Description and Significance

Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.

Genome Structure

Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.


Cell Structure, Metabolism and Life Cycle

Interesting features of cell structure; how it gains energy; what important molecules it produces.


Physiology and Pathogenesis

BIOCHEMICAL TEST RESULTS

  • Gram Reaction: positive
  • Capsule Stain: negative
  • Endospore Stain: negative
  • Motility: positive
  • Phenol Red Broth Tests: Glucose: yellowish-pink, negative; Lactose: red-pink, negative; Sucrose: red-pink, negative
  • Starch Hydrolysis Test: negative
  • Casein Hydrolysis Test: positive
  • Gelatin Hydrolysis Test: negative
  • DNA Hydrolysis Test: negative
  • Lipid Hydrolysis Test: negative
  • Methyl Red Test: negative
  • Voges Proskauer Test: negative
  • Citrate Test: positive
  • SIM Tests: negative for all
  • Nitrate Reduction: negative
  • Urea Hydrolysis: positive
  • Triple Sugar Iron Agar: negative for all
  • Oxidase Test: negative
  • Eosin Methylene Blue Agar (EMB) Test: positive
  • Hektoen Enteric Agar (HE) Test: green, negative, no growth
  • MacConkey Agar Test: negative
  • Decarboxylation Tests: Arginine: no change; Lysine: negative; Orinithine: negative
  • Phenylalanine Deaminase Test: negative
  • Catalase Test: negative
  • Blood Agar Test: negative
  • Mannitol Salt Agar (MSA) Test: negative
  • Phenylethyl Alcohol Agar (PEA) Test: negative
  • Bacitracin/Optochin Susceptibility Test: Bacitracin: non-resistant; Optochin: non-resistant
  • Bile Esculin Test: negative
  • 6.5% Salt Tolerance Test: slight positive

References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

Author

Page authored by Caylinda Miller and Maya Robinson, students of Prof. Kristine Hollingsworth at Austin Community College.