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This Glogowski Microbial Biorealm Genus template page is a guide for each team when adding information and your reference(s) to an already existing species on MicrobeWiki. DO NOT EDIT OR WORK DIRECTLY ON THIS TEMPLATE PAGE. COPY AND PASTE parts of this template that pertain to your information that you are submitting to the already existing species page and from there do your editing. Be sure to also copy and paste the bottom line provided on this template to add your team names on the line at the bottom of the species page that you edited after "Edited by" and in front of "students of M Glogowski at Loyola University". If the team is creating and adding an entirely new species to this website, COPY AND PASTE this entire template to set up the new page for that particular species, then begin to fill in and edit the page at that spot in MicrobeWiki and state at the bottom of the page that the new page is "Authored by [your Student Names]" in front of "students of M Glogowski at Loyola University".
Higher order taxa
Domain; Phylum; Class; Order; family [Others may be used. Use NCBI link to find]
Class: Beta Proteobacteria
Binomial name Ralstonia paucula
Description and significance
Describe the appearance, habitat, etc. of the organism, and why you think it is important. Ralstonia paucula sp. R. paucula has been isolated from pool water, groundwater, and bottled mineral water and from clinical specimens. Despite its low pathogenicity, it is now recognized as an opportunistic pathogen which can generate serious infections such as septicemia, peritonitis, abscess, and tenosynovitis, particularly in immunocompromised patients
16S Ribosomal RNA Gene Information
Describe the size and content of the 16S Ribosome. How many genes? Other interesting features? What is known about its sequence for identification? Ralstonia paucula PCR-ribotyping and PCR-restriction fragment length polymorphism analysis of the spacer 16S-23S ribosomal DNA (rDNA) has been used PCR-ribotyping was performed as described by Kostman et al. (7). The sequences of the 16S and 23S primers were 5'-TTGTACACACCGCCCGTCA-3' and 5'-GGTACCTTAGATGTTTCAGTTC-3' (7), respectively
Cell structure and metabolism
Interesting features of cell structure; how it gains energy; what important molecules it produces.
Habitat; symbiosis; contributions to the environment. R. paucula has been isolated from pool water, groundwater, and bottled mineral water (2, 9, 17) and from clinical specimens
How does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms. Ralstonia paucula (formerly CDC group IV c-2) has low pathogenicity, but it is now recognized as an opportunistic pathogen which can generate serious infections such as septicemia, peritonitis, abscess, and tenosynovitis, particularly in immunocompromised patients
Enter a summary of the most recent pertinent research here--at least one. Ralstonia paucula (Formerly CDC Group IV c-2): Unsuccessful Strain Differentiation with PCR-Based Methods, Study of the 16S-23S Spacer of the rRNA Operon, and Comparison with Other Ralstonia Species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum) Recently, several authors have reported the use of PCR-ribotyping (3, 5, 7, 8, 12, 22, 23) and PCR-restriction fragment length polymorphism (PCR-RFLP) analysis (20, 21) for strain differentiation within various bacterial species. the stydy we evaluated the ability to distinguish between R. paucula strains and between strains belonging to other Ralstonia species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum). As the ribosomal DNA (rDNA) intergenic spacer can contain genes coding for tRNA (15, 24), we also explored this region in R. paucula and compared it to those of other Ralstonia species.
It is required that you add at least one journal reference (in same format as the sample reference below) that corresponds to the info that you added to this page.
[Sample reference] Faller, A., and Schleifer, K. "Modified Oxidase and Benzidine Tests for Separation of Staphylococci from Micrococci". Journal of Clinical Microbiology. 1981. Volume 13. p. 1031-1035.
Edited by students of M Glogowskiat Loyola University 1- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC87738/