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===Higher order taxa===
===Higher order taxa===
Bacteria; Phylum; Class; Order; Family; Genus:


Bacteria; Phylum; Class; Order; Family; Genus
===Species===
Bacteroidete; Bacterodia; Bateroidates; Prevotellaceae; Prevotella; Prevotella amnii.


===Species===
Genus species:


Genus species
Prevotella amnii
==Description and significance==
==Description and significance==


The isolate of this species were found to live in human amniotic fluid. This specie is a rod shaped gram negative bacteria. It is anaerobic, non-spore forming, and non-motile. These cells are about 0.8- 3.0 by 0.5-1.5 µm in size and can be about 1 mm in diameter. These cells appear as white, smooth, and glistening.  
==Include as many headings as are relevant to your microbe (including things like cell metabolism, ecology, pathology, application to biotechnology). Or, if your microbe is very new and not well studied, then include a heading or two with more description about its native environment or something related to its lifestyle.==


==Cell metabolism==
After conducting some tests and the results show that some of the major end products of glucose metabolism were acetic acid, succinic acid, and small quantities of isovaleric acid, isocuproic acid, and lactic acid. There were also some quantities of fatty acids found like: C13 : 1 (0.4 %), C14 : 0 (0.9 %), iso-C14 : 0 (5.6 %), C15 : 0 (0.3 %), iso-C15 : 0 (16.4 %), anteiso-C15 : 0 (32.3 %), iso-3-OH-C15 : 0 (0.9 %), C16 : 0 (6.4 %), iso-C16 : 0 (9.7 %), 3OH-C16 : 0 (2.0 %), iso-C17 : 0 (3.4 %), anteiso-C17 : 0 (2.9 %), iso-3-OH-C17 : 0 (10.3 %), 2-OH-C17 : 0 (1.1 %), C18 : 0 (1.8 %) and C18 : 1v9c (2.9 %).
==Phylogenetic Affinities==
There were many species from the genus Prevotella analyized compared to Prevotella amnii to see the phylogenetic affinities. Prevotella amnii was determined to be closely related to the species Prevotella bivia, a species found to live in the endometrium and is commonly recovered from patients with pelvic inflammatory diseases. To determine the phylogenetic affinities, the 16S rRNA gene sequence was used and amplified by PCR.
==Biochemical characteristics==
Prevotella amnii can be distinguished from Prevotella bivia by its ability to produce phosphor-6-β-galactosidase, an enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. and its incapability to produce α-fucosidase and glutamyl-glutamic acid arylamidase, an enzyme that catalyzes the hydrolysis of the Glu dipeptide.
==Include as many headings as are relevant to your microbe (including things like cell metabolism, ecology, pathology, application to biotechnology).  Or, if your microbe is very new and not well studied, then include a heading or two with more description about its native environment or something related to its lifestyle.==


==Current Research==
==Current Research==
To identify this species, amniotic fluid was collected from a 29 year old female in 1999 and tested. The fluid was defined as turbid and foul smelling. In another study, amniotic fluid was also collected from a 26 year old female in 2006 and the samples was cultured on Columbia agar enhanced with 5% defibrinated horse blood at 37 ºC. Fermentation tests were performed on the samples.
Include information about how this microbe (or related microbes) are being studied and for what purpose
After identifying the species, some tests were done and samples were collected from healthy women and from women with pelvic disease. The results show that the organism is found to live in healthy women, but in some unique case, it can be found in some disease processes and should be considered as an emerging “opportunistic pathogen”. It’s also likely that some isolates formally associated with pelvic diseases were identified as Prevotella bivia, when in fact there were strains of Prevotella amnii. Now that can be corrected by looking at the biochemical characteristics of the two species


==References==
==References==
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]


Edited by (Nasra Ali!), student of Rachel Larsen at the University of Southern Maine
Edited by (Insert your name here), student of Rachel Larsen at the University of Southern Maine


<!--Do not edit or remove this line.-->[[Category:Pages edited by students of Rachel Larsen]]
<!--Do not edit or remove this line.-->[[Category:Pages edited by students of Rachel Larsen]]
"Beta-galactosidase." Princeton University. N.p., n.d. Web. 07 Mar. 2014.http://www.princeton.edu/~achaney/tmve/wiki100k/docs/Beta-galactosidase.html
"Glutamylglutamic Arylamidase | Online References | Cyclopaedia.net." Glutamylglutamic Arylamidase | Online References | Cyclopaedia.net. N.p., n.d. Web. 07 Mar. 2014. http://en.cyclopaedia.net/wiki/Glutamylglutamic-arylamidase
Lawson, Paul A. "International Journal of Systematic and Evolutionary Microbiology."Prevotella Amnii Sp. Nov., Isolated from Human Amniotic Fluid. IJSEM, Jan. 2008. Web. 07 Mar. 2014. http://ijs.sgmjournals.org/content/58/1/89.full
"Prevotella Amnii CRIS 21A-A — Classifications." Encyclopedia of Life. N.p., n.d. Web. 07 Mar. 2014.http://eol.org/pages/11781948/names

Latest revision as of 12:59, 17 October 2017

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Classification

Higher order taxa

Bacteria; Phylum; Class; Order; Family; Genus:

Species

Genus species:

Description and significance

Include as many headings as are relevant to your microbe (including things like cell metabolism, ecology, pathology, application to biotechnology). Or, if your microbe is very new and not well studied, then include a heading or two with more description about its native environment or something related to its lifestyle.

Current Research

Include information about how this microbe (or related microbes) are being studied and for what purpose

References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

Edited by (Insert your name here), student of Rachel Larsen at the University of Southern Maine