Tetrahymena thermophila: Difference between revisions

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==Ecology and Pathogenesis==
==Ecology and Pathogenesis==
''Tetrahymena thermophila'' has been found to have localized distribution within the geographic areas where it is found. "T. thermophilia" has been isolated in many states but are found mostly in fresh water ponds all over Easter North America. The organism tends to be more abundant in the months of June through September. It is also more likely found in areas that consist of decaying vegetation. They have been consistently isolated from ponds, particularly ponds that do not completely ice over during the winter months.   
''Tetrahymena thermophila'' has been found to have localized distribution within the geographic areas where it is found. ''T. thermophila'' has been isolated in many states but are found in fresh water ponds all over Eastern North America. The organism tends to be more abundant in the months of June through September. It is also more likely found in areas that contain decaying vegetation. They have been consistently isolated from ponds, particularly those that do not completely ice over during the winter months.   
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br>
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br>
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br>
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br>

Revision as of 04:31, 30 April 2015

This student page has not been curated.
Vegetative cell with macronucleus and micronucleus labeled with DAPI

Classification

Higher order taxa

Eukaryota; Chromalveolata; Ciliophora; Oligohymenophorea; Hymenostomatida; Tetrahymenidae

Species

Tetrahymena thermophila

Description and Significance

Tetrahymena thermophila is a large, motile, phagocytic, unicellular eukaryote. The organism is about 20x50µm. T. thermophila live in temperate freshwater environments.

Tetrahymena is an essential model organism that has been used in many biochemistry oriented experiments to identify biological phenomena. Currently this organism is being used to study four major issues: cilia biogenesis, telomerase structure and function, small RNA mediated self versus non-self discrimination, and epigenetic inheritance.

Describe the appearance, habitat, etc. of the organism, and why you think it is important.

Genome Structure

T. thermophila contain two nuclei, a macronucleus and a micronucleus. Each has been sequenced[1]. The macronucleus is ultimately derived from the micronucleus. The micronucleus is diploid and contains 5 pairs of chromosomes. The macronucleus contains 45 copies of 275 chromosomes formed by fragmentation, as well as 9000 copies of the rRNA gene. The macronucleus contains 15% less genetic information than the micronucleus, which represents noncoding DNA and transposable elements. The macronucleus is kept intact through asexual reproduction by telomerase activity. Additionally the mitochondrial genome has been sequenced [2].

Cell Structure

Schematic diagrams of the organization of Tetrahymena thermophila

Tetrahymena thermophila has all the basic structures of animal cells (ER, golgi, mitochondria, actin, tubulin etc.) except intermediate filaments. Additionally T. thermophila contain enlarged food vacuoles and a contractile vacuole which aids in regulating osmotic pressure. The cell has 18-21 rows of cilia lining its cell membrane. The cilia contain phosphonolipds which are resistant to degradation by phospholipase. Food enters the organism through the oral apparatus and exits through the cytoproct.

Metabolism

T. thermophila obtains energy through aerobic respiration. In lab T. thermophila's generation time is between 2-3 hours. It requires 11 essential amino acids, six B-complex vitamins, Fe3+ and trace metals. Additionally it is unable to synthesize purines and pyrimidines.

How it gains energy; what important molecules it produces.

Life Cycle

The presence of the two nuclei reflects T. thermophila’s ability to reproduce both sexually and asexually. During vegetative growth the macronucleus is transcribed, translated and transmitted to the next asexual generation by mitosis. Food stress induces T. thermophila to reproduce sexually, producing pronuclei by meiosis of the micronucleus and exchange with a cell of a different mating type. The macronucleus is ultimately derived from the micronucleus.

T. thermophila life cycle

Ecology and Pathogenesis

Tetrahymena thermophila has been found to have localized distribution within the geographic areas where it is found. T. thermophila has been isolated in many states but are found in fresh water ponds all over Eastern North America. The organism tends to be more abundant in the months of June through September. It is also more likely found in areas that contain decaying vegetation. They have been consistently isolated from ponds, particularly those that do not completely ice over during the winter months. Habitat; symbiosis; biogeochemical significance; contributions to environment.
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.

References

[3] DOERDER, F., ARSLANYOLU, M., SAAD, Y., KACZMAREK, M., MENDOZA, M., & MITA, B. (1996). Ecological Genetics of Tetrahymena thermophila: Mating Types, i‐Antigens, Multiple Alleles and Epistasis. Journal of Eukaryotic Microbiology, 43(2), 95-100.

Orias, E., et al. “Tetrahymena thermophila, a unicellular eukaryote with separate germline and somatic genomes”. Research in Microbiology. 2011. Volume 162. p. 578-586

Eisen, J., et al. “Macronuclear Genome Sequence of the Ciliate Tetrahymena thermophila, a Model Eukaryote”. Public Library of Science. 2006. Volume 4. p. 1620-1642.

Collins, K., and Gorovsky, M. "Tetrahymena thermophila". Current Biology. 2005. Volume 15. p. R317-R318.

Asai, D., and Forney, J. "Tetrahymena thermophila". 1999. Methods in Cell Biology. Volume 62. p. ii-xviii, 3-585.

Martindale, D., et al. "Conjugation in Tetrahymena thermophila". Experimental Cell Research. 1982. Volume 140. p. 227-236.

Author

Page authored by Hannah Pak and Luke Pryke, students of Prof. Jay Lennon at Indiana University.