The Human Gut: Difference between revisions

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These bacteria are also responsible for the breaking down of fiber, a very complex molecule that can be difficult even past the stomach. Some key molecules, such as vitamins, can be secreted by certain strains of bacteria.<br>
These bacteria are also responsible for the breaking down of fiber, a very complex molecule that can be difficult even past the stomach. Some key molecules, such as vitamins, can be secreted by certain strains of bacteria.<br>
Additionally, low levels of more harmful bacteria, such as Clostridium difficile can serve as a defense mechanism against foreign pathogens entering the GI tract, preventing infections.<br>
Additionally, low levels of more harmful bacteria, such as Clostridium difficile can serve as a defense mechanism against foreign pathogens entering the GI tract, preventing infections.<br>
[[Image:gut_microflora.jpg|thumb|400px|right|Representation of the role of the human gut microflora in regulating bodily functions, human health and disease. By Dr. Ana M Valdes at the University of Nottingham.]]


==Conclusion==
==Conclusion==

Revision as of 09:50, 12 June 2020

By Mia Tran

Overview

The human gut, also known as the gastrointestinal (GI) tract, consisting of all the organs that allow for humans to consume, digest, and expel food, and is highly populated by a diverse range of microbiota that serves many crucial processes in maintaining human health, such as digestion, fighting infection, and production of key vitamins and antioxidants.[1] The microbes occupying the gut ranges to the order of 1017[2] and consists primarily of bacteria, but also includes a variety of protozoa, archaea, prokaryotes, and viruses.[3]

Description of Physical and Biogeochemical Environment

Summary of GI tract pH gradients and corresponding communities. By S.M. Jandhyala et. al. at Department of Gastroenterology, Asian Institute of Gastroenterology, Asian Healthcare Foundation, Hyderabad, India.

The physical environment of the GI tract consists of an interface ranging from 250-400 square meters in an adult human.[4] The distribution of microbiota within this interface depends greatly on various gradients existing in the gut, such as nutrient and chemical gradients.[4] Microbes tend to sit either in the lumen or mucosa, and very rarely penetrates the bowel wall, as most microbes that achieve this tend to be pathogenic.[1]

The biogeochemical landscape of the gut various from organ to organ. The small intestine, the first organ in the tract following the stomach, is characterized by low pH, high oxygen, and an abundance of antimicrobials.[4] These conditions allow for limiting microbe growth, just so facultative anaerobes begin to settle to the mucus lining of the intestine, making way for an environment conducive for obligate anaerobes.[4] In the colon, or large intestine, there is a much more densely populated and diverse microbiota.[4] Communities of bacteroides, streptococci, and clostridia can outnumber facultative anaerobes up to a ratio of 1000:1.[1] The pH gradient and corresponding microbial communities are summarized in the figure to the right.[5]

Overview of History and Microbial Ecology

Relationship between human gut microbiome and cancer therapies. By H. Zitvogel et. al. at the Gustave Roussy Comprehensive Cancer Center, Villejuif, France.
Diversity of phyla and functions in human feces across individuals. By Dr. C.A. Lozupone et. al. at the Department of Chemistry and Biochemistry, University of Colorado, Boulder, USA.

Leading up to the formal study of the gut microbiota, breakthroughs in culturing anaerobes in 1944[6] and using a faecal transplant to treat a Clostridioides difficile infection in 1958[7] were some first breakthroughs in the field. The study of microflora in the human gut formally began in 1960s, where much of the initial research began with experiments using germ-free animals,[8] and studying the composition and count of microbes.[2] At the time, most of the work being done focused on strains that could be cultured. With technological developments in sequencing, scientists in the 1990s were able to identify and classify the makeup of the human microbiota more accurately than they were before when culture was the only method of exploration.[4][9] Since then, research exploded during the 2000s and 2010s due to the development of "omics" technology that allowed scientists to learn about communities that could not be cultured in the lab.[10]

Current research centers around pushing these "omics" methods and applying knowledge of the gut microbiome to human health.[11] As of late, cancer related research shows that the composition of gut microbiota can affect the efficacy of certain cancer treatments, and vice versa.[12] This bidirectional relationship is summarized in the figure to the left.[12] Much research has been conducted on how to manipulate the gut microbiota to aid in cancer therapy.[11]

When examining the diversity of the human GI tract from culture based techniques, it was thought that all adult humans share a common “core” microbiota. However, with developments beyond culture-based studies, it has been revealed that the human microbiome is so diverse across populations and times that the concept of a core ceases to exist.[13] In the average human stool sample, up to 400 species can be found, giving a rough estimate at species richness.[1] Despite extensive species diversity between individuals over space and time, there was significant functional redundancy between parts of the GI tract. This means that there are quite similar functional profiles across individuals, showcased in the figure to the right.[13]

Acquisition of the Gut Microbiome at Birth and Infancy

An individual's gut microbiome is first acquired at birth, not before. Babies are sterile within the womb, but at the moment of birth are exposed to bacteria from placenta tissue, umbilical blood, amniotic fluid, skin, and other membranes.[14] Natural and cesarean births can have different impacts on the acquired microbiome of the babies, as infants delivered vaginally will pick up microbes from the mother's vagina, and infants delivered through cesarean section will acquire microbes common to the mother's skin.[15]

Flow chart of influencing factors on human microbiome development through life. By Juan Miguel Rodríguez at the Complutense University of Madrid.

After birth, the composition of microbiota is quickly changing and developing.[15] The infant's diet of breastmilk or formula, as well as various environmental factors can shift the communities in its gut during the first few years of life. It is common for there to be initially low diversity, as the Proteobacteria and Actinobacteria phyla dominate, and facultative anaerobes populate the infant gut to shape the environment for other anaerobes. As the child matures, usually after the first year, the gut experiences higher diversity dominated by the phyla Firmicutes and Bacteroidetes.[14]

Factors Changing the Composition of Gut Microbiota During Adulthood

A number of factors can influence the composition of gut microbiota in an adult. The most influential is diet; studies examining factors such as location or seasonality usually will lead back to diet altering the gut microbiome. Adults with diets rich in fruits, vegetables, and fiber were found to have more diverse microbiomes.[5] The use of antibiotics has also shown to have potential effects on the GI tract's microbiome. Antibiotics can affect the gut in the short and long term; since the gut microbiome is quite resilient, short term use of antibiotics can wipe out some microbial populations, but some individuals are able to recover quickly. However, in some individuals, we can see that even short term use can alter diversity and population proportions for up to years afterwards.[5][16] Studies on germ free mice have shown a correlation between antibiotic treatments to incidence of type 1 diabetes, and in children a positive correlation between exposure to antibiotics and the development of asthma later in life; this indicates that antibiotics may impact gut communities so extensively, it affects the immune system significantly enough for long-term health conditions like diabetes and asthma to occur.[16] Use of probiotics and prebiotics, which are small doses of microorganisms that can benefit health and supplementary nutrients, respectively, are thought to add to communities that are considered "good" microbes.[5] Though many of these products have been readily available to the general public, further research should be conducted to confirm the real effectiveness of using probiotics.[5][13] Finally, studies between adult monozygotic and dizygotic twins show that they have equally similar microbiomes, indicating the influence of the general environment of upbringing rather than genetics can be attributed to microbial makeup.[13]

Dysbiosis: Health Impacts of Disrupted Microflora

A healthy gut microbiota can look different among different individuals. Major shifts in the composition of communities in one individual is known a dysbiosis, which is a condition that can lead to various types of health issues. There exists much literature on bacterial communities, but the spotlight has recently gone to fungi, viruses, and archaea. One of those most studied health impacts due to dysbiosis is obesity; studies of germ free mice shows that transplants of microbes from obese mice show a lack of expression of genes that encode for leptin, a hormones that inhibits hunger. Obese people have been found to have an increased amount of Firmicutes and decreased populations of Bacteroides. As described earlier, other immune-related health problems such as asthma, diabetes, and allergies have links to imbalances in the human GI tract microbiome.

Another major health problem that may arise from dysbiosis is IBS (inflammatory bowel syndrome) and Crohn's disease, which are intestinal disorders directly linked to the gut microbiome. This is a result of a general decrease in microbial populations within the gut, as well as a change in composition due to decreases in Firmucutes, increases in Bacteroids, and increases in facultative anaerobes.

Key Microbial Players

As mentioned before, the majority of the microbes in the human gut are bacteria belonging to the Firmicutes, then the Bacteroidetes phyla. A majority of the bacteria cannot be cultured, which is why the development of technologies such as 16s RNA sequencing has allowed the scientific community to unlock more about the composition of the gut microflora.
Within the gut, these bacteria are able to perform a variety of functions crucial to the human body. Enterohepatic circulation is mediated by these communities, which moves substances from the liver to bile, to the small intestine for absorption, then back to the liver; this overall serves the purpose of aiding digestion and absorption of substances like drugs and important acids.
These bacteria are also responsible for the breaking down of fiber, a very complex molecule that can be difficult even past the stomach. Some key molecules, such as vitamins, can be secreted by certain strains of bacteria.
Additionally, low levels of more harmful bacteria, such as Clostridium difficile can serve as a defense mechanism against foreign pathogens entering the GI tract, preventing infections.

Conclusion

(To be added)

References

(To be added)

  1. 1.0 1.1 1.2 1.3 Gorbach SL. "Microbiology of the Gastrointestinal Tract." In: Baron S, editor. Medical Microbiology. 4th edition. Galveston (TX): University of Texas Medical Branch at Galveston; 1996. Chapter 95.
  2. 2.0 2.1 Sender R., Fuchs S., and Milo R. "Revised Estimates for the Number of Human and Bacteria Cells in the Body." PLOS Biology 14(8): e1002533. 2016
  3. Kho Zhi Y., Lal Sunil K. "The Human Gut Microbiome – A Potential Controller of Wellness and Disease." Frontiers in Microbiology, Vol.9. 2018
  4. 4.0 4.1 4.2 4.3 4.4 4.5 Thursby, E., and Juge, N. "Introduction to the human gut microbiota" Biochemical Journal, Vol.474(11),1823-1836. 2017
  5. 5.0 5.1 5.2 5.3 5.4 Jandhyala, S.M., Talukdar, R., Subramanyam, C., Vuyyuru, H., Sasikala, M., and Reddy, D.N. "Role of the normal gut microbiota" World Journal of Gastroenterology : WJG, Vol.21(29), 8787-8803. 2015
  6. Clark, H. "Culturing anaerobes." Nature Research. 2019
  7. Stone, L. "Faecal microbiota transplantation for Clostridioides difficile infection." Nature Research. 2019
  8. Brunello, L. "Gut microbiota transfer experiments in germ-free animals." Nature Research. 2019
  9. Tang, L. "Sequence-based identification of human-associated microbiota." Nature Research. 2019
  10. [https://www-sciencedirect-com.turing.library.northwestern.edu/science/article/pii/S088240101530231X Hugon, P., Lagier, J.C., Colson, P., Bittar, F., and Raoult,D. "Repertoire of human gut microbes." Microbial Pathogenesis, Vol 106, 103-112. 2017]
  11. 11.0 11.1 Nature. "Human microbiota affects response to cancer therapy." Nature Research. 2019
  12. 12.0 12.1 [https://www-ncbi-nlm-nih-gov.turing.library.northwestern.edu/pmc/articles/PMC4690201/ Zitvogel, H. et al. "Cancer and the gut microbiota: An unexpected link." Science Translational Medicine, Vol 7(271). 2015]
  13. 13.0 13.1 13.2 13.3 [https://doi-org.turing.library.northwestern.edu/10.1038/nature11550 Lozupone, C., Stombaugh, J., Gordon, J. et al. "Diversity, stability and resilience of the human gut microbiota." Nature, Vol 489, 220-230. 2012]
  14. 14.0 14.1 Rodriguez, J.M. et al. "The composition of the gut microbiota throughout life, with an emphasis on early life." Microbial Ecology in Health and Disease, Vol 26. 2015
  15. 15.0 15.1 McBurney, M.I. et al. "Establishing What Constitutes a Healthy Human Gut Microbiome: State of the Science, Regulatory Considerations, and Future Directions." The Journal of Nutrition, Vol 149(11), 1882–1895. 2019
  16. 16.0 16.1 Cully, M. "Antibiotics alter the gut microbiome and host health." Nature. 2019



Authored for Earth 373 Microbial Ecology, taught by Magdalena Osburn, 2020, NU Earth Page.