Difference between revisions of "Thermoplasma acidophilum"
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the ''acidophilum''that .()
Tremendous amount of research had been made on 20S proteasome of ''T.acidophilum'', because this enzyme is common in higher eukaryotic organism and can play an important role on medical research. (12) Scientist are trying to find a way to deregulate this enzymatic system which might help to suppress or cure cancer.
Revision as of 02:34, 31 May 2007
A Microbial Biorealm page on the genus Thermoplasma acidophilum
Higher order taxa
Cellular organisms;Archaea; Euryarchaeota; Thermoplasmata; Thermoplasmatales; Thermoplasmataceae; Thermoplasma acidophilum (1)
Description and significance
Thermoplasma acidophilum is a thermophilic heterotrophic prokaryote, “they are among the most acidophilic organism known.” They grow at 55-60°C and favor a really low pH of 0.5-4; (2) It is found and first isolated from self-heating coal refuse piles and solfatara fields. (3)
Scientists have long been fascinated by the ability of this microorganism to grow at high temperatures and low pH, they are specially interest in the protein these organism have because common protein get denature under the acidic and hot environment without the structural protection of a conventional cell wall.
By determining and studying the genome sequence of this organism, we can have better understanding in protein folding and degrading which led to present a more complete representation of the proteins involved in different metabolic pathways. (3)
Thermoplasma acidophilum’s genome contain special gene that allow this organism to survive in an environment similar in temperature and acidity to hot vinegar. (4)
The genome of Thermoplasma is a single circular chromosome (1.56Mbp, containing 1509 ORFs)(4) and it is one of the smallest microbial genomes ever sequenced. (3)
Although the characteristics of Thermoplasma acidophilum’s small genome identify these organism as a“typical euryarchaeon with a substantial complement of bacterial-related genes.” However,massive lateral gene transfer appears to have occurred between Thermoplasma and Sulfolobus solfataricus, "a phylogenetically distant crenarchaeon inhabiting the same environment." (2)
Cell structure and metabolism
Species of the genus Thermoplasma lack rigid cell wall, but are only delimited by a plasma membrane, it is “devoid of protective outer shells (S-layer, cell wall), yet maintains a near-neutral cytosolic pH.” (4)
The lipid composition of T. acidophilum has brought to attentions in recent research and has been the subject of several investigations. This work has revealed the presence of “large polar lipids, often glycosylated with glucose, mannose and glucose, phosphorylated, or both, forming phosphoglycolipids” (5). These unusual lipid compositions allow T.acidophilum to survive under the harsh conditions of temperature and pH“restricting proton flow across the membrane more efficiently than non-branched lipids (6)”
Thermoplasma acidophilum is able to gain energy in several ways, either an aerobically by sulphur respiration or as a scavenger in extreme environment.
The Entner-Doudoroff pathway can only occur in prokaryotes like Thermoplasma. This pathway is a series of reactions that “catabolize glucose to pyruvate using a different set of enzymes other than those used in either glycolysis or the pentose phosphate pathway. Distinct feature of Entner-Doudoroff pathway is that it uses 6-phosphogluconate dehydrase and 2-keto-3-deoxyglucosephophate aldolase to create pyruvates from glucose." (14)
In Thermoplasma, glucose degradation is done by a “non-phosphorylated variant of the Entner–Doudoroff pathway, in which the first step is catalyzed by glucose dehydrogenase.” Acetyl-CoA produced from this pathway enters the TCA cycle. (the presence of enzyme require for this pathway had already been experimentally confirmed), the presence of glycolysis/gluconeogenesis has not yet been confirmed due to the absence of phosphofructokinase and fructose in this organism. (7)
Thermoplasma acidophilum plays an important role in the ecosystem, they act as scavenger of those extreme environment. “It has adapted to scavenging nutrients from the decomposition of organisms killed by the extreme acidity and requires yeast, bacterial or meat extract when grown in culture.” (3)
The study and research on Thermoplasma acidophilum indicate that an aqueous extract of coal refuse will serve as a nutrient source this is the first step leading to the discovery of the materials in coal refuse which support its growth. Indeed, the coal refuse material provides nutrients for the growth of a wide variety of microorganisms and could be a source of new growth factors. (8)
None of the archaea organism include Thermoplasma acidophilum had known to cause pathogen to human being.
Application to Biotechnology
The 20S proteasome from T.acidophilum has brought to attention by scientist recently because its proteolytic mechanism is common between the archaebacterial and the eukaryotic forms of the enzyme which is clinically relevant. (9)
In higher eukaryotes, proteasome is involved in housekeeping and protein level regulation; Further understand of chemical mechanism of this enzyme can help us to understand disease cause by the mutation of protesomes such as cystic fibrosis, Angelman's syndrome, Parkinson's disease, Liddle syndrome even cancer(10).
"Deregulation of this enzymatic system may also play a role in tumor progression, drug resistance, and altered immune surveillance, making the proteasome an appropriate and novel therapeutic target in cancer."(1) Proteasome also involve in apoptosis, which is a process to safely dispose of cell corpses and fragment. Apoptosis is a main type of programmed cell death, it plays a critical role in preventing cancer; if cell fails to undergo apoptosis, it can continue dividing and develop into tumor. (16)
“Large chunks of DNA which have been borrowed from other species of microbe and incorporated into the chromosome of acidophilum. The genes are believed to come from microbes and bacteria that share the same environmental niches, like the soil near hydrothermal sites.” (11) The main purpose of this kind of research is to have better understanding of how different microbe evolved and how these related to higher eukaryotes like humans.
The role of hydrophobic interactions in maintaining high temperature stability is investigated by citrate synthase of T.acidophilum as a thermostable model system. Scientist find out increasing or decreasing the hydrophobicity could further increase the thermostability of the T.acidophilum citrate synthase. Therefore, it is assumed that “interface substitutions affecting the entropy of the unfolded state did not prove to be so critical in protein thermostabilization at higher temperatures.” (17)
Tremendous amount of research had been made on 20S proteasome of T.acidophilum, because this enzyme is common in higher eukaryotic organism and can play an important role on medical research. (12) Scientist are trying to find a way to deregulate this enzymatic system which might help to suppress or cure cancer, because proteasome involve in apoptosis which plays a critical role in preventing cancer.
1. NEWT/ NCBI
2. Brock, T. D., in Thermoplilic microorganisms and life at high temperatures, pp. 92-116 (Springer, Berlin Heidelberg New York).
3. Don Cowan,Nature 407, 466-467,28 September 2000
4.Cedric F.V. Hobel, Sigillum Universitatis Islandiae, Access to Biodiversity and new genes from thermophiles by special enrichment methods, 2004
5. Budgen, N. & Danson, M. J. Metabolism of glucose via a modified Entner–Doudoroff pathway in the thermoacidophilic archaebacterium Thermoplasma acidophilum. FEBS Lett. 196, 207–210 (1986).
6. Gutsche, I. , Essen, L. O. & Baumeister, W. Group II chaperonins: New TRiC(k)s and Turns of a Protein Folding Machine. J. Mol. Biol. 293, 295– 312 (1999).
7.Searcy, D. G. & Whatley, F. R. Thermoplasma acidophilum: Glucose degradative pathways and respiratory activities. Syst. Appl. Microbiol. 5, 30–40 (1984).
8. Darland, G., Brock, T.D., Samsonoff, W. & Conti, S.F. A thermophilic acidophilic mycoplasm isolated from a coal refuse pile. Science 170, 1416-1418 (1970).
9. Zwickl, P. , Ng, D. , Woo, K. M. , Klenk, H. P. & Goldberg, A. L. An archaebacterial ATPase, homologous to ATPases in the eukaryotic 26 S proteasome, activates protein breakdown by 20 S proteasomes. J. Biol. Chem. 274, 26008– 26014 (1999).
10. Voges, D. , Zwickl, P. & Baumeister, W. The 26S proteasome: A molecular machine designed for controlled proteolysis. Annu. Rev. Biochem. 68, 1015–1068 (1999).
11.Bijal P. Trivedi, T. acidophilum living the hot, acidic life, Genome News Network Sep 29, 2000
12. Keiji Tanaka and Tomoki Chiba, The proteasome: a protein-destroying machine, The Tokyo Mtropolitan Institute of Medical Science, 1998
13. Wolf S, Lottspeich F and Baumeister W, Ubiquitin found in the archaebacterium Thermoplasma acidophilum, 1993 Jul 12
14. Mohamed, M. Optometry Professor. Smith, K. Optometrist. Sohn, S. Biomedical Science Professor.
15. J B Almond and G M Cohen, the proteasome: a novel target for cancer chemotherapy, Leukemia (2002) 16, 433-443. DOI: 10.1038/sj/leu/2402417
16. Murphy, KM et al (2000). "Bcl-2 inhibits Bax translocation from cytosol to mitochondria during drug-induced apoptosis of human tumor cells". Cell Death and Differentiation
--chwon 22:00, 29 April 2007 (UTC)
Edited by student of Rachel Larsen and Kit Pogliano