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[[Image:OULOGO1.JPEG|thumb|230px|left|University of Oklahoma Study Abroad Microbiology in Arezzo, Italy[http://faculty-staff.ou.edu/]]]
 
[[Image:Trichinella_larvae.jpeg‎|300px|thumb|right|Image of <i>Trichinella spiralis</i>. [http://en.wikipedia.org/wiki/Trichinella_spiralis#mediaviewer/File:Trichinella_larv1_DPDx.JPG]]]
[[Image:Trichinella_larvae.jpeg‎|300px|thumb|right|Image of <i>Trichinella spiralis</i>. [http://en.wikipedia.org/wiki/Trichinella_spiralis#mediaviewer/File:Trichinella_larv1_DPDx.JPG]]]


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===Description===
===Description===
The genus Trichinella is composed of eight species and three genotypes, all with the potential to infect hosts such as mammals, birds, and reptiles depending on pathogenic features. Biological testing was necessary in order to distinguish species due to highly similar morphological characteristics [[#References|[3]]]. In conjunction to (UPGMA)-based trees that provide accurate DNA results, species can only further be classified into two clades depending on the presence or absence of a collagen capsule surrounding the host striated muscles during infection. Trichinella spiralis is classified within the first clade, which presents the collagen capsule. In 2004, T.spiralis was one of eight non-mammalian organisms to be selected for full genetic sequencing. This was done in an effort to further investigate and understand the link between nematodes and other organisms under the animal kingdom. As a result of this research, it was discovered that T.spiralis is in some facets more genetically similar to other multicellular organisms within the Animalia kingdom than it was to nematodes.
The genus Trichinella is composed of eight species and three genotypes, all with the potential to infect hosts such as mammals, birds, and reptiles depending on pathogenic features. Biological testing was necessary in order to distinguish species due to highly similar morphological characteristics [[#References|[3]]]. In conjunction to (UPGMA)-based trees that provide accurate DNA results, species can only further be classified into two clades depending on the presence or absence of a collagen capsule surrounding the host striated muscles during infection. ''Trichinella spiralis'' is classified within the first clade, which presents the collagen capsule. In 2004, ''T.spiralis'' was one of eight non-mammalian organisms to be selected for full genetic sequencing. This was done in an effort to further investigate and understand the link between nematodes and other organisms under the animal kingdom. As a result of this research, it was discovered that ''T.spiralis'' is in some facets more genetically similar to other multicellular organisms within the Animalia kingdom than it was to nematodes.
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Trichinella spiralis is the smallest nematode pathogenic to humans, is present on all continents of the world except Antarctica, and has been found in 55 different countries across the globe [[#References|[4]]]. This zoonotic parasite causes systemic trichinosis, a gastrointestinal disease spread by the consumption of raw meat, specifically pork. The earliest known case of trichinosis was identified from an Egyptian mummy from 1300 BCE [[#References|[5]]]. Depending on the severity of the infection and location of the parasite, victims may be asymptomatic or they may experience diarrhea, pain, vomiting, swelling, fever, and rashes [[#References|[6]]].
''Trichinella spiralis'' is the smallest nematode pathogenic to humans, is present on all continents of the world except Antarctica, and has been found in 55 different countries across the globe [[#References|[4]]]. This zoonotic parasite causes systemic trichinosis, a gastrointestinal disease spread by the consumption of raw meat, specifically pork. The earliest known case of trichinosis was identified from an Egyptian mummy from 1300 BCE [[#References|[5]]]. Depending on the severity of the infection and location of the parasite, victims may be asymptomatic or they may experience diarrhea, pain, vomiting, swelling, fever, and rashes [[#References|[6]]].
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The male roundworm commonly grows between 1.4mm and 1.6mm while the ovo-viviparous female is generally twice as large and can reach up to a length of 3.2mm. This parasitic bilateral roundworm secretes a cuticle outer layer composed of collagen, which acts as a buffer against the immune response of the host. T.spiralis has the potential to enter three different life cycles based on the host. During the urban cycle, the parasite invades a pig, which is than transferred to a human host by consumption of undercooked meat. In the sylvatic cycle, other mammals serve as the host while whales and seals are the primary host target during the marine cycle [[#References|[7]]].
The male roundworm commonly grows between 1.4mm and 1.6mm while the ovo-viviparous female is generally twice as large and can reach up to a length of 3.2mm. This parasitic bilateral roundworm secretes a cuticle outer layer composed of collagen, which acts as a buffer against the immune response of the host. ''T.spiralis'' has the potential to enter three different life cycles based on the host. During the urban cycle, the parasite invades a pig, which is than transferred to a human host by consumption of undercooked meat. In the sylvatic cycle, other mammals serve as the host while whales and seals are the primary host target during the marine cycle [[#References|[7]]].
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==Pathogenesis==
==Pathogenesis==
===Transmission===
===Transmission===
The transmission of <i>Salmonella enterica</i> serovar Typhi, like most <i>Samonella</i> serovars, occurs through the fecal-oral route. The pathogen is  human host-adapted and generally spreads through contaminated food and water sources. Some hosts of <i>Salmonella</i> Typhi become asymptomatic carriers and can unknowingly transmit the pathogen to others. Because they are unaware of their condition, these carriers often lack proper preventative measures for slowing the spread of bacteria.  Without proper prevention, the bacteria are able to attain a high transmission rate. While lacking symptoms themselves, these carriers excrete large amounts of the bacteria in their feces and pass on the pathogen by contaminating food and water sources  [[#References|[10]]]. A primary example of an asymptomatic carrier who caused high transmission rates is "Typhoid Mary."  Mary Mallon, an Irish cook in the New York City area, was the first known typhoid carrier in the United States.  Despite her complete lack of symptoms, it is believed that Mallon caused a minimum of seven outbreaks of typhoid fever, resulting in 57 cases of the disease and 3 deaths [[#References|[11]]].
[[Image:Transmission_of_Trichinella_spiralis.jpeg|300px|thumb|left|Transmission of <i>Trichinella spiralis</i>. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2620635/figure/f1/]]]
 
Transmission of ''Trichinella spiralis'' only occurs through the consumption of animal meat infected with pathogenic cysts, encasing ''T.spiralis'' larvae. Natural hosts of the pathogen include rodents, bears, dogs, and even horses. Animals in close contact with rodents, such as domesticated hogs, are at the highest risk of contracting the infection [[#References|[8]]]. The worm is generally found in abundance on the tongue, diaphragm, and with less frequency in skeletal muscles [[#References|[9]]]. As a result of persistent nature of the pathogen, human contraction of Trichinosis is generally attributed to consumption of raw or undercooked domesticated swine infected with ''T.spiralis'' larvae. In Europe however, cases of trichinosis are caused by consumption of infected horses and wild boars [[#References|[10]]]. Infected meat would have been exposed to the worm for approximately two weeks in order for the parasite to grow and develop larvae. These larvae must also migrate to the striated muscles for further transmission into other susceptible hosts. Human to human transmission is not possible unless ingestion of contaminated human meat occurs [[#References|[11]]].
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===Infectious dose, incubation, and colonization===
===Infectious dose, incubation, and colonization===
The infectious dose is 10<sup>3</sup> to 10<sup>6</sup> bacilli, and the incubation period is between 7-14 days. <br><br>
The infectious dose of trichinosis is still unknown, however predictions estimate consuming between 100 and 300 of live cysts will result in the disease [[#References|[9]]]. A severe infection may result from the ingestion of 1,000 larvae, however these estimates have also not been scientifically proven [[#References|[10]]].
A high infectious dose of <i>Salmonella</i> Typhi is needed for infection, because it lacks acid tolerance. As a result much of the bacteria is destroyed as it passes through the highly acidic environment of the stomach. The bacteria must be abundant enough to overcome conditions of the stomach to successfully infiltrate the host [[#References|[6]]]. <br>
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To colonize, <i>S.</i> Typhi adheres to the mucosal lining of the small intestine and penetrates the epithelial cells. The bacteria spread to the peripheral lymphoid organs during secondary infection. The gallbladder serves as the primary reservoir of chronic infection. The formation of biofilms in the gut and on gallstones is a critical factor in the carriage and shedding of <i>S.</i> Typhi [[#References|[12]]].
Development and colonization of ''T. spiralis'' occurs entirely within the host during two larval stages and one adult stage [[#References|[9]]]. When a host ingests the pathogen, the larvae will spend between one to seven days developing into an adult in the gastrointestinal tract. Gastric symptoms of the disease will occur during this time frame. Incubation period of larvae in the host tissue may range from one week to eight weeks, however encapsulated larvae may persist in the host for months or even years. Incubation periods vary depending on the severity and initial infectious dose [[#References|[12]]].
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Initial colonization begins as the host ingests cysts containing larvae. During digestion, acidic compounds such as hydrochloric acid dissolve the cystic shell. The liberated larvae will pass through the stomach and invade and occupy epithelial tissues of the small intestine [[#References|[5]]]. Once established, the larvae will undergo four molting events to become an adult worm and mate. The female will potentially produce between 500-1,500 larvae before being expelled from the body in the stool [[#References|[3]]]. The larvae will then move across the intestinal wall and migrate to the rest host by lymphatic blood vessels, specifically the striated muscles. Exact mechanisms of this transfer across the intestine are unknown. Once ''T. spiralis'' larvae invade individual muscle cells, they adjust cellular function to accommodate their nutrient requirements thereby turning the host cell into a nurse cell. These mechanisms are still unknown today. The nurse cell will stop its lifecycle as the worm stimulates new blood vessel formation around the host cell for nutrients. ''T. spiralis'' larvae will also encapsulate itself during development [[#References|[5]]]. This incubation period generally lasts 15 days, however this is also based on severity of the infection. If left untreated, these larvae will remain in nurse cells for as long as 40 years [[#References|[10]]].
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===Epidemiology===
===Epidemiology===
Typhoid fever has continued to be a persistent threat in developing countries such as India, South American, Southeast Asia, and Africa due to poor sanitation conditions and lack of access to clean water [[#Reference|[13]]]. 80% of typhoid cases are believed to have originated from Bangladesh, India, China, Indonesia, Nepal, Laos, Vietnam, or Pakistan [[#Reference|[14]]]. The CDC reported that 22 million people are affected by <i>Salmonella enterica</i> serovar Typhi yearly, which resulted in approximately 200,000 deaths [[#Reference|[15]]]. The United States reports between 200 and 300 of these cases yearly. Approximately 80% of these cases are a consequence of traveling to countries with a high occurrence of typhoid fever [[#Reference|[16]]]. Before the use of antibiotics in the United States, the fatality rate was between 9-13%. Modern day medicine has decreased the mortality rate for patients that have access to treatment to just below one percent while mortality rates for patients that receive no treatment are greater than 10 percent [[#Reference|[3]]].
Outbreaks of trichinosis occur worldwide, however infections are is more common in Europe, Asia, and Southeast Asia. This disease is now endemic in Japan and China [[#References|[13]]]. Outbreaks are generally linked to cultural dietary preferences. The number of cases globally is estimated to be approximately 10,000, however these statistics are possibly higher due to unreported cases in countries without proper identification techniques. The mortality rate for trichinosis is approximately 0.2% [[#References|[14]]]. Rates of infection have dramatically decreased in the United States over the past decades. In the late 1940s, the CDC reported 400 cases of Trichinosis yearly. Based on statistics from 2010, the number of cases yearly has dropped to 20. This change can be attributed to increased awareness of the pathogen as well as strict regulations enacted about meat preparation as well the adoption of safer pig raising methods. Currently, most reported cases of trichinosis in the United States stem from personal preparation of wild game instead of commercial production error [[#References|[6]]].
 
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The World Health Organization claims that statistics are difficult to verify due to lack of proper supplies and measuring techniques in regions throughout Africa. However, they believe that the rate of typhoid fever infections has been gradually declining over the past several years in countries such as India and Chile. These results are attributed to increased attention towards health and sanitation methods [[#Reference|[17]]].  
Outbreaks have recently occurred in Europe within the last 20 years. In 2003, Poland experienced an outbreak that caused 124 people to be hospitalized. Romania experienced a similar outbreak in 2008 that caused 108 people to be hospitalized. These outbreaks were all linked to contaminated and undercooked meat [[#References|[9]]].
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===Virulence Factors===
===Virulence Factors===
Most of the bacterium's virulence arises from <i>Salmonella</i> pathogenicity islands, also known as SPI. These islands encode for the majority of effector molecules associated with pathogen virulence. For example, after entering a host cell, <i>Salmonella</i> Typhi will secrete effector proteins including SIPA and SptP. These proteins will alter the actin cytoskeleton of the host cell, which is responsible for cell migration [[#References|[6]]].<br>
[[File:Muscle_cells_containing_larvae_enclosed_by_nurse_cells.jpeg‎|400px|thumb|right|From: http://www.veterinaryresearch.org/content/42/1/113/figure/F4]]]
SPI7 is considered the most important pathogenicity island because it codes for the Vi antigen which is expressed on the surface. This antigen resides within a polysaccharide capsule which is essential for increased virulence and severity of symptoms. It is believed that this capsule also prevents lipopolysaccharide recognition by Pattern Recognition Receptors (PRRs) in order to prevent immune response; however, further research is required. Secretion of the protein invasin will allow non-phagocytic cells to ingest the bacterium in order to allow for intracellular access leading to the inhibition of oxidative leukocytes and rendering the innate immune response ineffective. Other possible factors include ion transporters, fimbrae, and flagella required for attachment and colonization [[#References|[6]]].
 
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The virulent nature of ''T. spiralis'' can be attributed to the capsule that surrounds the larvae during transmission as well as the high number of larvae a female produces in the intestine. The capsule is a distinguishing feature used to distinguish the two clades within the genus Trichinella [[#References|[3]]].
Recently, the typhoid toxin has been discovered. This toxin is known as chimaeric A2B5 typhoid toxin and is made up of two subunits including the PltA and CdtB. These two subunits are comparable to cytolethal and pertussis toxins. It is believed that this toxin is the cause of the high fevers present during the first and second week of infection [[#References|[18]]].
The capsule is believed to be an adaptive advantage of the pathogenic worm, allowing survival in high temperatures up to 70 degrees Celsius. The capsule also protects the nematode from freezing temperatures for extended time periods as well. Additional benefits of capsule include the ability to survive in dead and decaying animals for periods as long as four months. Other species of Trichinella that lack the capsule, such as ''T. papuae'', are only known to endure nine days within decomposing meat [[#References|[11]]].
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The defining characteristic of ''T.spiralis'' is the nurse cell that develops in the striated skeletal muscle. Direct damage of the host cell results from the infiltration of worm as well as the induced inflammatory response. The over production of host inflammatory cells produce an excess of reactive oxygen species causing oxidative stress in the cells and destroying homeostasis. The nurse cell is encompassed a capsule composed of collagen, which is secreted by the host cell. The creation of the capsule is known as cystogenesis.
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The exact mechanism used to construct the nurse cell continues to remain a mystery, however scientists theorize the host cell undergoes regeneration based on biochemical similarities between the two processes. During initial invasion of the parasite into host muscle cells, satellite cells located inside the capsule will be activated and begin proliferation. However, during this infection, the satellite cell will instigate the development of the parasitic nurse cell instead of maturing into a new muscle cell. This is caused by the interference of the notch signal pathway regulator proteins including MRF4. This de-differentiation of the satellite cell alters the morphology and genome of the host muscle cell, which constructs a suitable environment for parasitic growth [[#References|[15]]].
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In order to obtain nutrients, the parasite will provoke a hypotoxic event within the nurse cell complex, thereby evoking a vascular endothelial growth factor, also known as VEGF. This signal protein, usually stimulated in trauma to assist with wound healing, will stimulate blood vessel formation around the nurse cell. VEGF levels are highest during the incubation period of the larvae, particularly at day 15. Constant lower levels of this protein will also trigger increased vascular permeability and vessel dilation; therefore the nematode gains additional nutrients and disposes of waste faster [[#References|[16]]].
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Additional research suggests that ''T.spiralis'' secretes parakine molecules, similar to cytokines, in order to communicate with the host cell. This is essential to development of the nurse cell as well as the persistence of the pathogen inside. The worm can survive inside the nurse cell for up to 40 years because of signal secretion. In the event of nurse cell complex death, ''T. spiralis'' provokes the satellite cell to generate new nurse cells [[#References|[15]]].
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==Clinical Features==
==Clinical Features==
 
Severity and signs of symptoms vary on the number of larvae ingested by host. If the infection is minor based on the low amount of larvae ingested, ''T. spiralis'' may not be able to colonize and the immune system can free the infection. Symptoms may never appear or slowly intensify as larvae move to the muscles. If infectious dose is large enough, within the first week following infection gastrointestinal problems may arise including diarrhea, vomiting, cramps, or abdominal pain [[#References|[17]]]. As larvae migrate through the lymphatic system during the second week of infection, symptoms may include muscle pain, fever, swelling of the face or eyes, weakness, constipation or diarrhea, and splinter hemorrhages under the fingernail. These symptoms may persist for up to eight weeks with no medical attention, however larvae may survive in cells for up to 40 years [[#References|[18]]]. Many patients do not seek treatment due symptoms being similar to the flu.
Symptoms of the infection can be classified according to a time frame after exposure. During the incubation period of 7 to 14 days, the patient is asymptomatic as the bacteria colonizes and breaches the intestinal wall. Within 72 hours after the onset of the illness, the patient may experience a high fever between 103-104°F.
A rash on the abdomen or chest, fatigue, dry cough, and diarrhea also characterizes this first week of infection. If the patient has not received treatment by the second week, symptoms will increasingly worsen including the fever and continuation of diarrhea or constipation that can lead to extreme weight loss. By the third week with no medical attention, the patient will become delirious and experience severe exhaustion known as the typhoid state. During this week severe complications may occur in association with prolonged infection of typhoid fever [[#References|[19]]]. Approximately three percent of people with typhoid fever develop a perforated intestine causing internal bleeding which may cause blood to appear in the stool [[#References|[4]]].  Perforation results in a hole in the small or large intestine triggering symptoms of abdominal pain, nausea, vomiting, and blood sepsis.  Other complications associated with typhoid fever include myocarditis, intravascular coagulation, kidney or bladder infections, and meningitis. Psychiatric problems for example, delirium, hallucinations, and paranoid psychosis can also occur as a result of infection [[#References|[19]]]. If the patient is able to survive to the fourth week, they will gradually improve and are able to regain their mental state. However, this recovery period may last up to several weeks or months [[#References|[5]]]. Statistically, around 10 percent of patients will experience a relapse of symptoms after they have recovered. The probability of relapse is shown to increase with antibiotic usage. Three to five percent of survivors may also become long-term asymptomatic carriers with the potential to trigger new outbreaks [[#References|[20]]].
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Severe trichinosis may also become extremely debilitating. Loss of motor functions including walking, swallowing, and breathing can result from this agonizing pain [[#References|[19]]].
These effects can be more severe or prolonged in children and the elderly. Bacteremia, or the spread of the pathogen into the blood stream, generally occurs in 5-10% of cases and can lead to more severe symptoms such as meningitis and infections of the bones and joints. This can be especially dangerous in immunocompromised patients such as those suffering from HIV or Malaria [[#References|[6]]].  
In the case of severe trichinosis, complications may arise because migrating larvae have access to the entire body through the blood stream. ''T. spiralis'' larvae stimulate inflammation at major organ sites including the brain, lungs, and the heart. This may result in life threatening conditions such as myocarditis, encephalitis, meningitis, nephritis, pneumonia, or bronchopneumonia [[#References|[17]]].
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==Diagnosis==
==Diagnosis==
Stool cultures are used to diagnose the disease, but in many cases blood cultures must also be used to reach a confident diagnosis due to the sensitivity of stool cultures in the early and late stages of illness.  The blood cultures from the infected host can be tested on MacConkey and EMB agar [[#References|[18]]] [[#References|[21]]].
Diagnosis of the parasite during the first week of infection is challenging due to the similar secretion of enzymes, including creatine kinase and lactate dehydrogenase, which also elevate during other infections. Levels of eosinophil cells also elevate, however this is also nonspecific to trichinosis and could indicate other parasitic infections or even allergies.  
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<i>Salmonella</i> Typhi enters the bloodstream upon infection and is carried by white blood cells to the liver, spleen, and bone marrow where multiplication occurs, after which the bacteria enter back into the bloodstream. As the bacteria invade the lymphatic tissue of the bowel, they proliferate. The bacteria then enter the intestinal tract and can be used in the diagnosis of stool cultures from the laboratory. In early and late stages of the disease, stool cultures are more sensitive to culturing but should be tested simultaneously with blood culture to make a definitive diagnosis. Additional testing may be done to differentiate between the different serovars of <i>S. enterica</i> species. These molecular biological tests are based on different antigens on the bacteria's surfaces, such as O, K, and H [[#References|[3]]].
Detection of antibodies developed to this parasite through tests, such as indirect immunofluorescence and latex agglutination, are the least invasive tests available. A muscle biopsy, however, is the most effective testing in diagnosing trichinosis. The deltoid muscle is most commonly used to test for ''T.spiralis'' larvae formation. The biopsy can also indicate the severity and stage of the disease. Complications with this test include an incubation period between 17 to 24 days while the larvae develop, as well as a false negative test if infection rates are too low [[#References|[19]]].


==Treatment==
==Treatment==
Antibiotic therapy, specifically ciprofloxacin and ampicillin, is the only effective treatment. For pregnant women, ceftriaxone is used. Recently, antibiotic resistance by <i>S.</i> Typhi has increased and developed into a more serious issue concerning the effectiveness and use of antibiotics. To stimulate recovery, fluids and a healthy diet can be administered in addition to antibiotics [[#References|[21]]]. Previously, antibiotic treatment for typhoid fever included regimens of ampicillin, trimthoproim-sulfamethoxazole, and chloramphenicol. Usage of these drugs is now limited due to developing drug resistance over the past twenty years. Strains specific to South America have shown significant resistant to antibiotic therapy.  Currently, quinolone, macrolide, and third-generation cephalosporin antibiotics are used to treat resistant strains. Quinolone sensitivity has steadily declined in different parts of the world, but it remains significant in the United States [[#References|[13]]].
Depending on the severity of the infection, trichinosis may not require medical intervention. Moderate to severe infections require medication. Within the first week of infection, the main goal of medical intervention is to limit the spread of the larvae which would lead to a systemic infection. Patients are generally administered albendazole, mebendazole, or thiabendazole, an anti-parasitic medication. This treatment is less effective after larvae invasion of the muscles. If this occurs, pain relievers may be prescribed for sore muscles or antipyretics to counteract fever. The main purpose for medication after larvae have migrated is to control and decrease muscle tissue damage.
To treat the carrier state, prolonged antibiotics are prescribed.  Additionally, the direct site of chronic infection can be removed, such as gall bladder removal [[#References|[22]]]. <br>
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In a case where this infection results in an allergic reaction due to chemicals being released in the muscles after larvae death, corticosteroids are usually prescribed. While steroids are helpful in regards to controlling inflammation, they may delay the expulsion of the adult worms in the intestinal lining which would result in a longer infection [[#References|[5]]], [[#References|[17]]].


==Prevention==
==Prevention==
To avoid infection, hygiene such as clean hands and treated water is encouraged. Boiling water and correct procedure when handling raw fruits and vegetables decrease the risk of infection [[#References|[22]]]. In association, two vaccines are available: inactivated typhoid vaccine administered via injection and live typhoid vaccine administered orally. Neither vaccine is 100% effective, and both require repeated immunizations [[#References|[15]]]. <br>
Due to meat being the main vector of transfer, meat preparation must be monitored and controlled. This would require careful preparation of meat as well as animal feed which would include heating to higher temperatures to ensure larvae death. According to standards set by the USDA, a food thermometer should be used when cooking whole cut meats excluding wild game chicken in order to ensure the temperature reaches 145° F. This would also require a rest time of three minutes where the meat should be set out to before consumption in order to kill all pathogens as the temperature remains constant. For ground meat and wild that also excludes poultry, the meat should be cook above 160° F with no rest time required afterwards. Poultry should be cooked above 165 °F with a three minute rest time before consumption [[#References|[6]]].
To prevent the infection while recovering from typhoid, one should avoid handling food, isolate personal items, wash hands as often as possible, and clean toilets, door handles, and telephone receivers daily [[#References|[22]]]. <br>
Regulations about freezing meats are also in place. Deep freezing meat for three weeks is shown to deactivate larvae, however larvae in bear meat are more resistant to this method. Other methods of preserving meat including curing or smoking will not ensure the meat is pathogen free. Heating meat is the most effective way to ensure larval death [[#References|[12]]].
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Animals destined for human consumption should be carefully managed to ensure they are not in contact with wild animals such as rats, which would put them at risk of contracting trichinosis.


==Host Immune Response==
==Host Immune Response==
[[File:Salmonella Typhi Pathogenic Pathway.jpeg|400px|thumb|right|From: ncbi.nlm.nih.gov [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3478240/figure/F2/]]]
Trichinosis is transmitted through the consumption of ''T. spiralis'' larvae. These larvae are encapsulated, however as they reach progress through the digestive system, the capsule will dissolve. These larvae will settle in the duodenum and jejunum of the small intestine by breaching epithelial cells. An increase of cells responsible for allergic response is seen here including the rise of mucosal mast cells, eosinophils as well as IgE production [[#References|[19]]]. IgE production is hypothesized to be triggered by the pathogen in order to evade mast cells and inhibit binding of allergen specific IgE. T helper 2 cells are the main effector cells against this pathogen. As the larvae develops in the epithelial cells, CD4 T cells will also secrete cytokines such as IL-4 and IL-10 in the lymph nodes. T helper 2 cells will commonly be present during this response, and is helpful in repairing or preventing tissue damage caused by the worm. As larvae migrate into the host skeletal cells, the pathogen will induce an inflammatory response in which myopathy will occur due to the high levels of inflammatory cells producing toxic reactive oxygen species [[#References|[5]]].
 
<i>Salmonella enterica</i> serovar Typhi can cause life-threatening bacterial infections called typhoid fever. The uncontrolled activation of the host innate immune response can potentially lead to systematic inflammation, tissue injury, intravascular coagulation, and even death [[#References|[12]]]. <br><br>
<i>Salmonella enterica</i> serovar Typhi is an invasive pathogen. It is recognized by the host’s immune system using toll-like receptors (TLRs) which initiate the innate immune response. The TLRs recognize pathogen-associated molecular patterns (PAMPS) located on the surface of the pathogen. This recognition allows for the innate immune system to initiate its response, causing the activation and recruitment of macrophages, neutrophils, and cytokines. For example, IFN-γ is a significant cytokine for macrophage activation and early host resistance of <i>S.</i> Typhi [[#References|[12]]].<br><br>
Victims of typhoid fever are susceptible to reinfection because of the initial severe disruption of the gut microbiome. A typical host contains a microbiome of 1x10<sup>14</sup> bacteria with an average of 500 to 1,000 different species. A healthy microbiome can protect the host’s epithelial cells from infection. The gut microbiome produces toxic metabolites that can suppress the virulence of <i>S.</i> Typhi’s gene expression, boost the host’s immune response, and help clear the intestinal lumen after non-typhoidal diarrhea. Additionally, apoptosis can strengthen the host’s defense by allowing the body to prevent further release of pro-inflammatory cell mediators. The ability of <i>S.</i> Typhi to use a microbiome nutrient called ethanolamine allows it to colonize in the intestinal tract.  This rich nutrient often allows <i>S.</i> Typhi to outcompete other pathogens. Antimicrobial treatment for <i>S.</i> Typhi may cause depletion of the host’s gut micro biome, which can lead to prolonged effects of intestinal colonization and an increase in carrier status and fecal shedding [[#References|[12]]].
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==References==
==References==


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<br> 6 [http://www.cdc.gov/parasites/trichinellosis/epi.html "Parasites-Trichinellosis" CDC.]  
<br> 6 [http://www.cdc.gov/parasites/trichinellosis/epi.html "Parasites-Trichinellosis" CDC.]  
<br> 7 [http://animaldiversity.ummz.umich.edu/accounts/Trichinella_spiralis/ Hartwell, G. "Trichinella spiralis."]
<br> 7 [http://animaldiversity.ummz.umich.edu/accounts/Trichinella_spiralis/ Hartwell, G. "Trichinella spiralis."]
 
<br>8 [http://www.vdh.virginia.gov/Epidemiology/factsheets/pdf/Trichinellosis.pdf "Trichinellosis (Trichinosis)" Virginia Department of Health.]
<br>8 [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3478240/ Garai, P., Gnanadhas, D.P., Chakravortty, D. "Salmonella enterica serovars Typhimurium and Typhi as model organisms." US National Library of Medicine.]  
<br>9 [http://www.foodsafetywatch.org/factsheets/trichinella/ Lawley, R. "Trichinella" Food Safety Watch]  
<br>9 [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC151493/ Deng, W., Liou, S., Plunkett, G., et al. "Comparative Genomics of Salmonella enterica Serovar Typhi Strains Ty2 and CT18." US National Library of Medicine.]  
<br>10 [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2620635/ Gottstein, B., Pozio, E., Nockler, K. "Epidemiology, Diagnosis, Treatment, and Control of Trichinellosis" Clinical Microbiology Reviews. NCBI]
<br>10 [http://www.ncbi.nlm.nih.gov/pubmed/22591832 Gopinath, S., Carden, S., Monach, D. "Shedding light on Salmonella carriers." Trends Microbiology.]
<br>11 [http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/trichinella-eng.php Public Health Agency of Canada.]
<br>11 [http://ajph.aphapublications.org/doi/abs/10.2105/AJPH.29.1.66 "Mary Mallon (Typhoid Mary)." American Journal of Public Health.]
<br> 12 [http://www.novatec-id.com/produkte/novalisa/wuermer/trichinella-spiralis/NovaTec.]
<br> 12 [http://www.plospathogens.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1002933 de Jong, H.K., Parry, C.M., van der Pol, T. "Host-Pathogen Interaction in Invasive Salmonellosis" PLOS Pathogens.]
<br> 13 [http://web.stanford.edu/class/humbio103/ParaSites2005/Trichinella/trich.html "Trichinosis."]
<br> 13 [http://emedicine.medscape.com/article/228174-treatment Klochko, A. "Salmonellosis Treatment and Management." Medscape.]
<br> 14 [http://faculty.uml.edu/rhochberg/hochberglab/Courses/Parasite/PDF%20Papers/Nematodes/World%20Distribution%20of%20Trichinella.pdf "Trichinosis (Also known as Trichinellosis)."]
<br> 14 [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2167998/ Chau, T.T., Campbell, I.J. "Antimicrobial Drug Resistance of Salmonella enterica Serovar Typhi in Asia and Molecular Mechanism of Reduced Susceptibility to the Fluoroquinolones." NCBI.]
<br> 15 [http://www.parasitesandvectors.com/content/1/1/27 Takahashi, Y. "Trichinella spiralis: nurse cell formation with emphasis on analogy to muscle cell repair" Parasites and Vectors.]
<br> 15 [http://www.cdc.gov/nczved/divisions/dfbmd/diseases/typhoid_fever/ "Typhoid Fever" Center for Disease Control and Prevention.]
<br> 16 [http://lineu.icb.usp.br/~farmacia/artigos/Tspiralis_rev.PDF Despommier, D.D. "How Does Trichinella spiralis Make Itself at Home?"]
<br> 16 [http://www.uptodate.com/contents/epidemiology-microbiology-clinical-manifestations-and-diagnosis-of-typhoid-fever Hohmann, E.L., "Epidemiology, microbiology, clinical manifestations, and diagnosis of typhoid fever" UpToDate]
<br> 17 [http://www.mayoclinic.org/diseases-conditions/trichinosis/basics/complications/con-20027095 "Diseases and Conditions Trichinosis" Mayo Clinic.]
<br> 17 [http://www.who.int/rpc/TFDisBurden.pdf Crump, J.A., Luby, S.P., Mintz, E.D., "The global burden of typhoid fever" WHO]
<br> 18 [http://www.medicinenet.com/trichinosis/article.htm Davis, C. P. "Trichinosis" Medicinenet.]
<br> 18 [http://www.nature.com/nature/journal/v499/n7458/full/nature12377.html Song, J., Gao, X., Galan J.E. "Structure and Function of the <i>Salmonella</i> Typhi chimaeric A2B5 typhoid toxin" Nature: International weekly journal of science.]
<br> 19 [http://pmj.bmj.com/content/78/915/15.full#sec-8 Bruschi, F., Murrell, K.D. "New aspects of human trichinellosis: the impact of new Trichinella species" Postgraduate Medical Journal.]
<br> 19 [http://www.mayoclinic.org/diseases-conditions/typhoid-fever/basics/definition/con-20028553 "Diseases and Conditions: Typhoid Fever" Mayo Clinic.]
<br> 20 [http://www.webmd.com/a-to-z-guides/typhoid-fever "Typhoid Fever." WebMD.]
<br> 21 [http://web.uconn.edu/mcbstaff/graf/Student%20presentations/Salmonellatyphi/Salmonellatyphi.html Pollack, D.V. "Salmonella enterica Typhi." University of Connecticut.]
<br> 22 [http://www.onhealth.com/typhoid_fever/page2.htm Balentine, J.R. "Typhoid Fever." Medicine Net.]
<br> 23 [http://www.nlm.nih.gov/medlineplus/ency/article/001332.htm "Typhoid Fever." Medline Plus.]
 




<br>
<br>
Created by Sarah Grebennikov
Created by Sarah Grebennikov
<br> Students of Dr. Tyrrell Conway, University of Oklahoma
<br> Student of Dr. Tyrrell Conway, University of Oklahoma

Latest revision as of 18:57, 11 February 2016

This is a curated page. Report corrections to Microbewiki.
University of Oklahoma Study Abroad Microbiology in Arezzo, Italy[1]
Image of Trichinella spiralis. [2]

Etiology/Bacteriology

Taxonomy

| Domain = Eukaryota | Kingdom = Animalia | Phylum = Nematoda | Class = Adenophorea | Subclass = Enoplia | Order = Trichocephalida | Family = Trichinellidae | Genus = Trichinella | species = T. spiralis

NCBI: Taxonomy Genome: [4]

Description

The genus Trichinella is composed of eight species and three genotypes, all with the potential to infect hosts such as mammals, birds, and reptiles depending on pathogenic features. Biological testing was necessary in order to distinguish species due to highly similar morphological characteristics [3]. In conjunction to (UPGMA)-based trees that provide accurate DNA results, species can only further be classified into two clades depending on the presence or absence of a collagen capsule surrounding the host striated muscles during infection. Trichinella spiralis is classified within the first clade, which presents the collagen capsule. In 2004, T.spiralis was one of eight non-mammalian organisms to be selected for full genetic sequencing. This was done in an effort to further investigate and understand the link between nematodes and other organisms under the animal kingdom. As a result of this research, it was discovered that T.spiralis is in some facets more genetically similar to other multicellular organisms within the Animalia kingdom than it was to nematodes.

Trichinella spiralis is the smallest nematode pathogenic to humans, is present on all continents of the world except Antarctica, and has been found in 55 different countries across the globe [4]. This zoonotic parasite causes systemic trichinosis, a gastrointestinal disease spread by the consumption of raw meat, specifically pork. The earliest known case of trichinosis was identified from an Egyptian mummy from 1300 BCE [5]. Depending on the severity of the infection and location of the parasite, victims may be asymptomatic or they may experience diarrhea, pain, vomiting, swelling, fever, and rashes [6].

The male roundworm commonly grows between 1.4mm and 1.6mm while the ovo-viviparous female is generally twice as large and can reach up to a length of 3.2mm. This parasitic bilateral roundworm secretes a cuticle outer layer composed of collagen, which acts as a buffer against the immune response of the host. T.spiralis has the potential to enter three different life cycles based on the host. During the urban cycle, the parasite invades a pig, which is than transferred to a human host by consumption of undercooked meat. In the sylvatic cycle, other mammals serve as the host while whales and seals are the primary host target during the marine cycle [7].

Pathogenesis

Transmission

Transmission of Trichinella spiralis. [3]

Transmission of Trichinella spiralis only occurs through the consumption of animal meat infected with pathogenic cysts, encasing T.spiralis larvae. Natural hosts of the pathogen include rodents, bears, dogs, and even horses. Animals in close contact with rodents, such as domesticated hogs, are at the highest risk of contracting the infection [8]. The worm is generally found in abundance on the tongue, diaphragm, and with less frequency in skeletal muscles [9]. As a result of persistent nature of the pathogen, human contraction of Trichinosis is generally attributed to consumption of raw or undercooked domesticated swine infected with T.spiralis larvae. In Europe however, cases of trichinosis are caused by consumption of infected horses and wild boars [10]. Infected meat would have been exposed to the worm for approximately two weeks in order for the parasite to grow and develop larvae. These larvae must also migrate to the striated muscles for further transmission into other susceptible hosts. Human to human transmission is not possible unless ingestion of contaminated human meat occurs [11].

Infectious dose, incubation, and colonization

The infectious dose of trichinosis is still unknown, however predictions estimate consuming between 100 and 300 of live cysts will result in the disease [9]. A severe infection may result from the ingestion of 1,000 larvae, however these estimates have also not been scientifically proven [10].

Development and colonization of T. spiralis occurs entirely within the host during two larval stages and one adult stage [9]. When a host ingests the pathogen, the larvae will spend between one to seven days developing into an adult in the gastrointestinal tract. Gastric symptoms of the disease will occur during this time frame. Incubation period of larvae in the host tissue may range from one week to eight weeks, however encapsulated larvae may persist in the host for months or even years. Incubation periods vary depending on the severity and initial infectious dose [12].

Initial colonization begins as the host ingests cysts containing larvae. During digestion, acidic compounds such as hydrochloric acid dissolve the cystic shell. The liberated larvae will pass through the stomach and invade and occupy epithelial tissues of the small intestine [5]. Once established, the larvae will undergo four molting events to become an adult worm and mate. The female will potentially produce between 500-1,500 larvae before being expelled from the body in the stool [3]. The larvae will then move across the intestinal wall and migrate to the rest host by lymphatic blood vessels, specifically the striated muscles. Exact mechanisms of this transfer across the intestine are unknown. Once T. spiralis larvae invade individual muscle cells, they adjust cellular function to accommodate their nutrient requirements thereby turning the host cell into a nurse cell. These mechanisms are still unknown today. The nurse cell will stop its lifecycle as the worm stimulates new blood vessel formation around the host cell for nutrients. T. spiralis larvae will also encapsulate itself during development [5]. This incubation period generally lasts 15 days, however this is also based on severity of the infection. If left untreated, these larvae will remain in nurse cells for as long as 40 years [10].

Epidemiology

Outbreaks of trichinosis occur worldwide, however infections are is more common in Europe, Asia, and Southeast Asia. This disease is now endemic in Japan and China [13]. Outbreaks are generally linked to cultural dietary preferences. The number of cases globally is estimated to be approximately 10,000, however these statistics are possibly higher due to unreported cases in countries without proper identification techniques. The mortality rate for trichinosis is approximately 0.2% [14]. Rates of infection have dramatically decreased in the United States over the past decades. In the late 1940s, the CDC reported 400 cases of Trichinosis yearly. Based on statistics from 2010, the number of cases yearly has dropped to 20. This change can be attributed to increased awareness of the pathogen as well as strict regulations enacted about meat preparation as well the adoption of safer pig raising methods. Currently, most reported cases of trichinosis in the United States stem from personal preparation of wild game instead of commercial production error [6].
Outbreaks have recently occurred in Europe within the last 20 years. In 2003, Poland experienced an outbreak that caused 124 people to be hospitalized. Romania experienced a similar outbreak in 2008 that caused 108 people to be hospitalized. These outbreaks were all linked to contaminated and undercooked meat [9].

Virulence Factors

]

The virulent nature of T. spiralis can be attributed to the capsule that surrounds the larvae during transmission as well as the high number of larvae a female produces in the intestine. The capsule is a distinguishing feature used to distinguish the two clades within the genus Trichinella [3]. The capsule is believed to be an adaptive advantage of the pathogenic worm, allowing survival in high temperatures up to 70 degrees Celsius. The capsule also protects the nematode from freezing temperatures for extended time periods as well. Additional benefits of capsule include the ability to survive in dead and decaying animals for periods as long as four months. Other species of Trichinella that lack the capsule, such as T. papuae, are only known to endure nine days within decomposing meat [11].

The defining characteristic of T.spiralis is the nurse cell that develops in the striated skeletal muscle. Direct damage of the host cell results from the infiltration of worm as well as the induced inflammatory response. The over production of host inflammatory cells produce an excess of reactive oxygen species causing oxidative stress in the cells and destroying homeostasis. The nurse cell is encompassed a capsule composed of collagen, which is secreted by the host cell. The creation of the capsule is known as cystogenesis.

The exact mechanism used to construct the nurse cell continues to remain a mystery, however scientists theorize the host cell undergoes regeneration based on biochemical similarities between the two processes. During initial invasion of the parasite into host muscle cells, satellite cells located inside the capsule will be activated and begin proliferation. However, during this infection, the satellite cell will instigate the development of the parasitic nurse cell instead of maturing into a new muscle cell. This is caused by the interference of the notch signal pathway regulator proteins including MRF4. This de-differentiation of the satellite cell alters the morphology and genome of the host muscle cell, which constructs a suitable environment for parasitic growth [15].

In order to obtain nutrients, the parasite will provoke a hypotoxic event within the nurse cell complex, thereby evoking a vascular endothelial growth factor, also known as VEGF. This signal protein, usually stimulated in trauma to assist with wound healing, will stimulate blood vessel formation around the nurse cell. VEGF levels are highest during the incubation period of the larvae, particularly at day 15. Constant lower levels of this protein will also trigger increased vascular permeability and vessel dilation; therefore the nematode gains additional nutrients and disposes of waste faster [16].

Additional research suggests that T.spiralis secretes parakine molecules, similar to cytokines, in order to communicate with the host cell. This is essential to development of the nurse cell as well as the persistence of the pathogen inside. The worm can survive inside the nurse cell for up to 40 years because of signal secretion. In the event of nurse cell complex death, T. spiralis provokes the satellite cell to generate new nurse cells [15].

Clinical Features

Severity and signs of symptoms vary on the number of larvae ingested by host. If the infection is minor based on the low amount of larvae ingested, T. spiralis may not be able to colonize and the immune system can free the infection. Symptoms may never appear or slowly intensify as larvae move to the muscles. If infectious dose is large enough, within the first week following infection gastrointestinal problems may arise including diarrhea, vomiting, cramps, or abdominal pain [17]. As larvae migrate through the lymphatic system during the second week of infection, symptoms may include muscle pain, fever, swelling of the face or eyes, weakness, constipation or diarrhea, and splinter hemorrhages under the fingernail. These symptoms may persist for up to eight weeks with no medical attention, however larvae may survive in cells for up to 40 years [18]. Many patients do not seek treatment due symptoms being similar to the flu.
Severe trichinosis may also become extremely debilitating. Loss of motor functions including walking, swallowing, and breathing can result from this agonizing pain [19]. In the case of severe trichinosis, complications may arise because migrating larvae have access to the entire body through the blood stream. T. spiralis larvae stimulate inflammation at major organ sites including the brain, lungs, and the heart. This may result in life threatening conditions such as myocarditis, encephalitis, meningitis, nephritis, pneumonia, or bronchopneumonia [17].

Diagnosis

Diagnosis of the parasite during the first week of infection is challenging due to the similar secretion of enzymes, including creatine kinase and lactate dehydrogenase, which also elevate during other infections. Levels of eosinophil cells also elevate, however this is also nonspecific to trichinosis and could indicate other parasitic infections or even allergies.

Detection of antibodies developed to this parasite through tests, such as indirect immunofluorescence and latex agglutination, are the least invasive tests available. A muscle biopsy, however, is the most effective testing in diagnosing trichinosis. The deltoid muscle is most commonly used to test for T.spiralis larvae formation. The biopsy can also indicate the severity and stage of the disease. Complications with this test include an incubation period between 17 to 24 days while the larvae develop, as well as a false negative test if infection rates are too low [19].

Treatment

Depending on the severity of the infection, trichinosis may not require medical intervention. Moderate to severe infections require medication. Within the first week of infection, the main goal of medical intervention is to limit the spread of the larvae which would lead to a systemic infection. Patients are generally administered albendazole, mebendazole, or thiabendazole, an anti-parasitic medication. This treatment is less effective after larvae invasion of the muscles. If this occurs, pain relievers may be prescribed for sore muscles or antipyretics to counteract fever. The main purpose for medication after larvae have migrated is to control and decrease muscle tissue damage.

In a case where this infection results in an allergic reaction due to chemicals being released in the muscles after larvae death, corticosteroids are usually prescribed. While steroids are helpful in regards to controlling inflammation, they may delay the expulsion of the adult worms in the intestinal lining which would result in a longer infection [5], [17].

Prevention

Due to meat being the main vector of transfer, meat preparation must be monitored and controlled. This would require careful preparation of meat as well as animal feed which would include heating to higher temperatures to ensure larvae death. According to standards set by the USDA, a food thermometer should be used when cooking whole cut meats excluding wild game chicken in order to ensure the temperature reaches 145° F. This would also require a rest time of three minutes where the meat should be set out to before consumption in order to kill all pathogens as the temperature remains constant. For ground meat and wild that also excludes poultry, the meat should be cook above 160° F with no rest time required afterwards. Poultry should be cooked above 165 °F with a three minute rest time before consumption [6]. Regulations about freezing meats are also in place. Deep freezing meat for three weeks is shown to deactivate larvae, however larvae in bear meat are more resistant to this method. Other methods of preserving meat including curing or smoking will not ensure the meat is pathogen free. Heating meat is the most effective way to ensure larval death [12].
Animals destined for human consumption should be carefully managed to ensure they are not in contact with wild animals such as rats, which would put them at risk of contracting trichinosis.

Host Immune Response

Trichinosis is transmitted through the consumption of T. spiralis larvae. These larvae are encapsulated, however as they reach progress through the digestive system, the capsule will dissolve. These larvae will settle in the duodenum and jejunum of the small intestine by breaching epithelial cells. An increase of cells responsible for allergic response is seen here including the rise of mucosal mast cells, eosinophils as well as IgE production [19]. IgE production is hypothesized to be triggered by the pathogen in order to evade mast cells and inhibit binding of allergen specific IgE. T helper 2 cells are the main effector cells against this pathogen. As the larvae develops in the epithelial cells, CD4 T cells will also secrete cytokines such as IL-4 and IL-10 in the lymph nodes. T helper 2 cells will commonly be present during this response, and is helpful in repairing or preventing tissue damage caused by the worm. As larvae migrate into the host skeletal cells, the pathogen will induce an inflammatory response in which myopathy will occur due to the high levels of inflammatory cells producing toxic reactive oxygen species [5].

References

1 University of Oklahoma Faculty and Staff.
2 Kenyan Waterborne Disease Center
3 Mitreva, M., Jasmer, P.D. "Biology and genome of Trichinella spiralis
4 Pozioa, E., Hobergb, E., Rosaa, G. L., Zarlengab, D.S. "Molecular taxonomy, phylogeny and biogeography of nematodes belonging to the Trichinella genus"
5 Smith, D.S. "Trichinosis".
6 "Parasites-Trichinellosis" CDC.
7 Hartwell, G. "Trichinella spiralis."
8 "Trichinellosis (Trichinosis)" Virginia Department of Health.
9 Lawley, R. "Trichinella" Food Safety Watch
10 Gottstein, B., Pozio, E., Nockler, K. "Epidemiology, Diagnosis, Treatment, and Control of Trichinellosis" Clinical Microbiology Reviews. NCBI
11 Public Health Agency of Canada.
12 [5]
13 "Trichinosis."
14 "Trichinosis (Also known as Trichinellosis)."
15 Takahashi, Y. "Trichinella spiralis: nurse cell formation with emphasis on analogy to muscle cell repair" Parasites and Vectors.
16 Despommier, D.D. "How Does Trichinella spiralis Make Itself at Home?"
17 "Diseases and Conditions Trichinosis" Mayo Clinic.
18 Davis, C. P. "Trichinosis" Medicinenet.
19 Bruschi, F., Murrell, K.D. "New aspects of human trichinellosis: the impact of new Trichinella species" Postgraduate Medical Journal.



Created by Sarah Grebennikov
Student of Dr. Tyrrell Conway, University of Oklahoma