Trypanosoma brucei

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A Microbial Biorealm page on the genus Trypanosoma brucei

Classification

Higher order taxa

Kingdom: Eukaryota; Phylum: Euglenozoa; Order: Kinetoplastida; Family: Trypanosomatidae; Genus: Trypanosoma; SubGenus: Trypanozoon; Species: Trypanosoma brucei

Species

Genus: Trypanosoma Species: brucei Sub-species: Trypanosoma brucei brucei,Trypanosoma brucei gambiense, Trypanosoma brucei rhodesiense,Trypanosoma brucei TREU927.

Description and Significance

The parasitic eukaryote, Trypanosoma brucei, is one of the species from the Trypanosoma genus. It exists in two forms: an insect vector, and once inside the bloodstream, a mammalian host. T. brucei exists as its insect vector in the tsetse fly, a large, biting fly originating in Africa. Once the tsetse fly bites a mammal, the microbe enters the bloodstream where it transforms into the mammalian host form, and is then capable of mutating and invading the central nervous system, (CNS). Once inside the CNS, it has the ability to inflict African trypanosomiasis, (sleeping sickness).

The complete genome of T. brucei has been sequenced; this is important because it is key information that is used to research possible cures for African trypanosomiasis.


Describe the appearance, habitat, etc. of the organism, and why it is important enough to have its genome sequenced. Describe how and where it was isolated. Include a picture or two (with sources) if you can find them.

Genome structure

Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence?Does it have any plasmids? Are they important to the organism's lifestyle?


The genome of T. brucei has 11 megabased-size chromosomes, ranging from one to six Mbp's, which do not condense during mitosis.(6)It is predicted to have 9068 genes, with about 904 pseudogenes, and 1700 genes that are specific for T. brucei. The G+C content is 46.4%.

The genome of T. brucei also has surface antigens that allow the bacteria to escape from being noticed by the immune system.(1) T. brucei is capable of continuously changing the expression of these antigens to effectively hide from antibodies. It is thought that there are around 806 VSG's in the genome. The mechanism for antigenic variation is under parasitic genetic control. (9) The microbe can release many different pathogenic substances, that cause changes in the cytokine/prostoglandin network. These changes can activate macrophages, which then release tumor necrosis factor and nitrous oxide. These substances enhance immunosuppression, and alter the blood brain barrier so that trypansomes and inflammatory cells can invade the CNS.

The circular mitochondrial genome in T. brucei is enclosed a single kintetoplast, which is positioned at the base of the flagellum.(7) The mitochondrial DNA is kinetoplasts is circular, and interwoven, similar to the World Olympic symbol. The circles exist in two sizes: mini and maxi. There are fewer maxi circles than there are mini circles, because of size. T. brucei has about 100 minichromosomes as well.

The cytoskeleton of T. brucei can be divided into three main classes: microtubules, intermediate filaments, and actin microfilaments.(6)

Cell structure and metabolism

In general, the cellular structure of T. brucei is similar to all other eukaryotes. There are however, a few differences. T. brucei's cell surface has, (in addition to its surface antigens), a thick layer of proteins, called Variant Surface Glycoprotein (VSG's) genes. These allow the surface antigens to mutate, by switching variants.(2) Having over 1000 VSG genes and psuedogenes, T. brucei is able to switch variants frequently. Trascription occurs one gene at a time, from one of many telomeric VSG expression sites.(3) In order to switch an active VSG gene, DNA rearrangements must occur, to switch the old VSG gene with a new one. Using the bloodstream form of T. brucei, scientists in the Netherlands discovered that telomere exchange, thought to be rare, was indeed occuring. The scientists marked a VSG gene with a hygromycin resistance gene, allowed the gene to undergo variation, and selected switched Trypanosomes. The drug sensitivity and polymerase chain reactions (PCR), revealed that telomere exchange had taken place.(4) Interestingly enough, of the 806 VSG's in the genome of T. brucei, only about 7% of them are thought to be fully functional!

T. brucei also has unusual Citric Acid Cycles and a single large mitochondria. In the insect vector host, the Citric Acid Cycle is not used to generate energy; rather parts of the Citric Acid Cycle are suggested to be used for: acetyl-CoA transport into cytosol, degradation of proline and glutamate to succinate, and the formation of malate(5) The Citric Acid Cycle is not functioning as a cycle itself, but parts of its pathways are being used in T. brucei.

It is very important to understand the cell's metabolism, as it is a key target for new drug synthesis. Most of the research done on T. brucei's metabolism is on the microbe's lifecycle; however, T. brucei has shown to be a the most metabolically restrictive species of the Trypanosoma genus.(6) It is thought that horizontal gene transfer from bacteria to the Tritryp lineage is the cause of this versatility. The Tritryp lineage has many essential genes that are required for the uptake/degredation of glucose. By targeting drugs to alter the pathways that use glucose, (glycolysis, the Citric Acid Cycle, or the pentose phosphate shunt), one could potentially discover new medicines for African Trypanosomiasis. Although about 50% of the genes of T. brucei have no known function, as of yet, many more biochemical pathways have yet to be discovered.

Cells usually use Type I or II synthase to undergo fatty acid synthesis. However, T. brucei has provided a third mechanism. T. brucei uses elongases to synthesize fatty acids. Other cells use elongases to make long-chain fatty acids even longer. The nature of this pathway allows synthesis of different fatty-acid end products(15), that have important roles in T. brucei's biology.

Ecology

Describe any interactions with other organisms (included eukaryotes), contributions to the environment, effect on environment, etc.


Insect-borne diseases can have profound affects on human life, agriculture, and livestock. African trypanosomiasis affects just these. T. brucei lives in the gut of the tsetse fly, along with two other symbionts: Sodalis glossinidius and Wigglesworthia glossinidia. (10) These other symbionts provide nutrients that the fly is either lacking in its diet, or incapable of synthesizing itself. They allow the host to live in normally unviable niches. All together, this provides a productive environment for T. brucei to thrive in.

Pathology

How does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.

T. brucei resides in the tsetse flys salivary glands, as metacyclic trypomastigotes. Once the fly bites a mammal, (human or animal), the metacyclic trypomastigotes get injected into the lyphatic system, and eventually pass into the bloodstream, where they transform into bloodstream trypomastigotes.(8) There, they reproduce asexually and go through many rounds of differentiation to adapt to the drastic changes in its environment. For example, the shape of T. brucei changes from short and stumpy, to long and slender. They then travel to different regions of the body, such as: lymph, body fluid, and the CNS. Once in the CNS, T. brucei causes an irreversible demyelinating process, due to an autoimmune response.(9) It can also cause swelling of the lymph nodes in the neck, the first sign of African trypanosomiasis.


The lifecycle of T. brucei.


It is hard to determine whether or not there are trypanosomes in the blood, because of the VSG's. To further determine if there are indeed trypanosomes in the blood, a spinal tap may be carried out to test spinal fluid, or bone marrow extractions may be needed.

Common symptoms of African trypanosomiasis are headaches, fevers, and abnormal behavior. The disease can induce coma, or even cause death.(8)

Treatment of T. brucei infection, or trypanosomiasis, is carried out with an array of different drugs. Prescription depends on what stage of trypanosomiasis the individual is in. For early stages, when trypansomes are only detected in the circulation, Pentamidine or Suramin are suggested. Melarsoprol may be used at all stages of trypanosomiasis, but a safer and newer drug has been developed, Eflornitine, and is used more often.

Application to Biotechnology

Does this organism produce any useful compounds or enzymes? What are they and how are they used?

One of T. brucei's VSG's have an enzyme called glycosyl-phosphatidylinositol (GPI). An inositol glycan fragment from GPI was analyzed to see if it mimics the effects of insulin antilipolytic activity. This specific fragment inhibits isoproterenol-stimulated lipolysis, which insulin also inhibits. Glycan-induced antilipolysis is blocked by a low Km cAMP phosphodiesterase ihibitor, imazodan, and okadaic acid. This fragment also causes inhibition of glucose-6-phosphatase and cytosolic fructose-1,6-bisphosphatase activity (essential enzymes in glycolysis/gluconeogenesis). The direct addition of the fragment to hepatocytes caused marked inhibition of gluconeogenesis. This suggests that the direct modification of the activities of these two enzymes by the inositol glycan fragment could play a role in the inhibition of glucose output by insulin, and provide the evidence for the insulin-mimetic properties of the inositol glycan fragment. (11)

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Current Research

Because T. brucei causes diseases, pathogenic parasites are constantly being studied. They are very toxic and have high resistance to current chemotherapeutics-an evergrowing issue in todays world. Today, chemotherapy is the main source of treatment against African trypanosomiasis.


A study was recently done on 18 structurally diverse bis-acridine compounds, against seven protozoan parasite species. The bioactivities and cytotoxicities of these compounds towards mammalian cells, were compared. When comparing the structure-activity, bis-acridine and acridine heterocycles showed an influence on cytotoxicity. They required a specific length of approximately 10 Å. When the length was increases, the activity of the parasites decreased, as well as a decrease in cytotoxicity towards mammalian cells. Several other similar tests were carried out, such as substitutions in N alkylations with different linkers, or changing the terminal compounds. The strongest compounds recorded low- to mid-nanomolar EC50 values against T. brucei. (12) Compound bioactivity was found to not be a function inhibition of trypanothione reductase, an important drug target in trypanosomatid parasites. This study demostrated the usefulness of screening bis-acridine compounds against parasitic targest, which can aid in the discovery of new chemotherapeutics-especially needed in Africa to counteract the effects of T. brucei's pathogenicity.


Other research is being conducted on heat shock therapies against T. brucei. In T. brucei, three mitogen-activated protein kinase (MAPK) pathways (which mediate developmental processes induced by stress/external signals), have been analyzed. TbMAPK4, on chromosome six, is a subclass of TbECK1. The function of TbMAPK4 is unknown, so to analyze it, null mutants of a monomorphic stock and of a pleomorphic stock were developed and tested. The various bloodstream forms of the two stocks were transformed using MAPK4-hyg. A hygromycin-resistant clone was then transformed again, and southern blot analysis showed that both copies of TbMAPK4 were deleted in one clone. This meant that null forms were able to grow normally. The optimal growth temperatures of the clones, 25 °C, was raised to 27 or 37 °C. Within 24 hours of the raise in temperature, the null mutants started to die. After 100 hours, all cells were dead. (13) The null mutants were much more heat shock sensitive than the parental lines. This could be explained because the null mutants might be unable to induce expression of heat shock proteins. O(13) Another transformed line of TbMAPK4, HA-TbMAPK4 was tested at different temperatures. Expression of TbMAPK4 protein was always lower in cells cultured at 37 °C, but not in cells cultured at 27 °C. In fact, the cells cultured at 27 °C showed a 2 fold increase in kinase activity. Scientists believe that because the cells were still able to grow in temperatures that the insect host normally inhabited, ~25 to 27°C, this meant that TbMAPK4 was not necessary for essential growth/differentiation processes in T. brucei. (13)


Continuous research on the drugs used to target trypanosomiasis is underway. T. brucei, shows resistance to tryparsamide. Many theories are being tested to explain the reason why drug resistance by T. brucei occurs. (14) Some of the theories include:

-Aquaglyeroporins

-Thiamine transporters

-High affinity pentamidine transporter (HAPT1)

-ABC transporters

References

Edited by Shannon Chan

(1)Frank SA, Barbour AG. "Within-host dynamics of antigenic variation". 'Infection Genetics and Evolution'. 2006. p. 146-146.

(2)Lythgoe KA, Morrison LJ, Read AF, Barry JD. "Parasite-intrinsic factors can explain ordered progression of trypanosome antigenic variation". 'Proceedings of The National Academy of Sciences of The United States of America'. 2007 May 8. p. 8095-8100.

(3)Taylor JE, Rudenko G. "Switching trypanosome coats: what's in the wardrobe?". 2006 Aug 14.

(4)Rudenko G, McCulloch R, Dirks-Mulder A, Borst P. "Telomere exchange can be an important mechanism of variant surface glycoprotein gene switching in Trypanosoma brucei". 1996 Sep.

(5)van Hellemond JJ, Opperdoes FR, Tielens AG. "The extraordinary mitochondrion and unusual citric acid cycle in Trypanosoma brucei". 2005 Nov.

(6)Berriman M, Ghedin E, Hertz-Fowler C, Blandin G, Renauld H, Bartholomeu DC, Lennard NJ, Caler E, Hamlin NE, Haas B, Böhme U, Hannick L, Aslett MA, Shallom J, Marcello L, Hou L, Wickstead B, Alsmark UC, Arrowsmith C, Atkin RJ, Barron AJ, Bringaud F, Brooks K, Carrington M, Cherevach I, Chillingworth TJ, Churcher C, Clark LN, Corton CH, Cronin A, Davies RM, Doggett J, Djikeng A, Feldblyum T, Field MC, Fraser A, Goodhead I, Hance Z, Harper D, Harris BR, Hauser H, Hostetler J, Ivens A, Jagels K, Johnson D, Johnson J, Jones K, Kerhornou AX, Koo H, Larke N, Landfear S, Larkin C, Leech V, Line A, Lord A, Macleod A, Mooney PJ, Moule S, Martin DM, Morgan GW, Mungall K, Norbertczak H, Ormond D, Pai G, Peacock CS, Peterson J, Quail MA, Rabbinowitsch E, Rajandream MA, Reitter C, Salzberg SL, Sanders M, Schobel S, Sharp S, Simmonds M, Simpson AJ, Tallon L, Turner CM, Tait A, Tivey AR, Van Aken S, Walker D, Wanless D, Wang S, White B, White O, Whitehead S, Woodward J, Wortman J, Adams MD, Embley TM, Gull K, Ullu E, Barry JD, Fairlamb AH, Opperdoes F, Barrell BG, Donelson JE, Hall N, Fraser CM, Melville SE, El-Sayed NM. "The genome of the African trypanosome Trypanosoma brucei". Wellcome Trust Sanger Institute. 2005 Jul 15.

(7)Eva Gluenz, Michael K. Shaw, Keith Gull. "Structural asymmetry and discrete nucleic acid subdomains in the Trypanosoma brucei kinetoplast". 2007. Molecular Microbiology 64 (6), p. 1529–1539

(8)"Parasites and health". 2005 May.

(9)Dumas M, Bouteille B. "[Human African trypanosomiasis". Institut d'Epidémiologie Neurologique et de Neurologie Tropicale. 1996.]

(10)Serap Aksoy, Rita V.M. Rio. "Interactions among multiple genomes: Tsetse, its symbionts and trypanosomes". Department of Epidemiology and Public Health, Yale University School of Medicine. 2005 Feb.


(11)DE Misek and AR Saltiel. "An inositol phosphate glycan derived from a Trypanosoma brucei glycosyl- phosphatidylinositol mimics some of the metabolic actions of insulin". University of Michigan Medical School. 1992 Aug.

(12)Conor R. Caffrey, Dietmar Steverding, Ryan K. Swenerton, Ben Kelly, Deirdre Walshe, Anjan Debnath, Yuan-Min Zhou, Patricia S. Doyle, Aaron T. Fafarman, Julie A. Zorn, Kirkwood M. Land, Jessica Beauchene, Kimberly Schreiber, Heidrun Moll, Alicia Ponte-Sucre, Tanja Schirmeister, Ahilan Saravanamuthu, Alan H. Fairlamb, Fred E. Cohen, James H. McKerrow, Jennifer L. Weisman, and Barnaby C. "Bis-Acridines as Lead Antiparasitic Agents: Structure-Activity Analysis of a Discrete Compound Library In Vitro". 2007 Mar.

(13)Andreas Güttingera, Claudia Schwaba, Sabine Moranda, Isabel Roditia and Erik Vassellaa. "A mitogen-activated protein kinase of Trypanosoma brucei confers resistance to temperature stress". 2007 Feb.

(14)Vincent Delespauxa and Harry P. de Koning. "Drugs and drug resistance in African trypanosomiasis". 2007 April.