User:Hmcclend: Difference between revisions

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==Genome structure==
==Genome structure==
 
The replicon number and sizes vary from strain to strain in Burkholderia cepacia species.  The largest replicon is found in strain N2P5 (9.3 Mb).  Most species contain 2 to 4 large replicons, and many also contain smaller replicons as well.  Many species contain plasmids and, all species have circular chromosomes.  The Burkholderia cepacia type- strain ATCC 25416 (genomovar I) is 8.1 Mb in length and is known to have four circular replicons.  Its largest replicon contains 4 rrn operons and the other two megabase- sized replicons contain a single rrn operon each.  From this information it can be inferred that the organism has three chromosomes and one large plasmid. (6)


==Cell structure and metabolism==
==Cell structure and metabolism==

Revision as of 01:35, 3 June 2007

A Microbial Biorealm page on the genus Hmcclend

Classification

Higher order taxa

Bacteria; Proteobacteria; Betaprotebacteria; Burkholderiales; Burkholderiaceae; Burkholderia

Species

NCBI: Taxonomy

Burkholderia cepacia

Description and significance

Burkholderia cepacia was first described by Walter Burkholder of Cornell University in 1949 when he determined it to be the cause of bacterial rot of onion bulbs. It was originally named Pseudomonas cepacia and was later changed to its current name. Burkholderia cepacia refers to a complex of nine closely related species listed above. They are rod-shaped, free-living, motile Gram- negative bacteria ranging from 1.6- 3.2 μm. They have been found to possess multitrichous polar flagella as well as pili used for attachment. Burkholderia cepacia can be found in soil, water, and infected plants, animals, and humans. Aside from being a plant and human pathogen it has many significant agricultural uses. It is capable of breaking down toxic compounds found in pesticides and herbicides. It has also been noted to repress certain soil-borne pathogens and is being considered as an agent for promoting crop growth.

The appearance of Burkholderia cepacia species varies based on the strain and the culture medium used. Three media are currently being used to isolate the bacteria. They are the following: Pseudomonas cepacia agar (PCA), oxidation fermentation polymyxin bacitracin lactose agar (OFBL), and Burkholderia cepacia selective agar (BCA). The last medium has proved to be the most effective since it actually suppresses the growth of non-Burkholderia cepacia bacteria. Burkholderia cepacia bacteria will form visible pinpoint colonies within 24 hours, and the colonies appear to be smooth and somewhat elevated.

Genome structure

The replicon number and sizes vary from strain to strain in Burkholderia cepacia species. The largest replicon is found in strain N2P5 (9.3 Mb). Most species contain 2 to 4 large replicons, and many also contain smaller replicons as well. Many species contain plasmids and, all species have circular chromosomes. The Burkholderia cepacia type- strain ATCC 25416 (genomovar I) is 8.1 Mb in length and is known to have four circular replicons. Its largest replicon contains 4 rrn operons and the other two megabase- sized replicons contain a single rrn operon each. From this information it can be inferred that the organism has three chromosomes and one large plasmid. (6)

Cell structure and metabolism

Ecology

Pathology

Application to Biotechnology

Current Research

Enter summaries of the most recent research here--at least three required

References

Edited by student of Rachel Larsen and Kit Pogliano