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<b><i>Ralstonia paucula</i></b> | |||
==Classification== | |||
===Higher order taxa=== | |||
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find] | |||
===Species=== | |||
[ | {| | ||
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'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]''' | |||
|} | |||
Ralstonia paucula | ''<i>Ralstonia paucula</i>'' | ||
==Description and significance== | |||
<i>Ralstonia paucula</i> is a Gram-negative environmental bacterium. | |||
Isolated from pool water, groundwater, bottled mineral water, and many clinical specimens. Ralstonia paucula, despite its low pathogenicity, is now recognized as an opportunist pathogen that can generate serious infections, such as septicemia, peritonitis, abscesses, etc., especially in immunocompromised patients. | |||
==16S Ribosomal RNA Gene Information== | |||
In 1995, bacteria was confronted with five cases of nosocomial bacteremia, we found that pulsed-field gel electrophoresis could not distinguish between the isolates and that randomly amplified polymorphic DNA analysis was poorly discriminatory. In this study, we used PCR-ribotyping and PCR-restriction fragment length polymorphism analysis of the spacer 16S-23S ribosomal DNA (rDNA); both methods were unable to differentiate R. paucula isolates. Eighteen strains belonging to other Ralstonia species (one R. eutropha strain, six R. pickettii strains, three R. solanacearum strains, and eight R. gilardii strains) were also tested by PCR-ribotyping, which failed to distinguish between the four species. The 16S-23S rDNA intergenic spacer of R. paucula contains the tRNAIle and tRNAAla genes, which are identical to genes described for R. pickettii and R. solanacearum. [1] | In 1995, bacteria was confronted with five cases of nosocomial bacteremia, we found that pulsed-field gel electrophoresis could not distinguish between the isolates and that randomly amplified polymorphic DNA analysis was poorly discriminatory. In this study, we used PCR-ribotyping and PCR-restriction fragment length polymorphism analysis of the spacer 16S-23S ribosomal DNA (rDNA); both methods were unable to differentiate R. paucula isolates. Eighteen strains belonging to other Ralstonia species (one R. eutropha strain, six R. pickettii strains, three R. solanacearum strains, and eight R. gilardii strains) were also tested by PCR-ribotyping, which failed to distinguish between the four species. The 16S-23S rDNA intergenic spacer of R. paucula contains the tRNAIle and tRNAAla genes, which are identical to genes described for R. pickettii and R. solanacearum. [1] | ||
==Cell structure and metabolism== | |||
Interesting features of cell structure; how it gains energy; what important molecules it produces. | |||
==Ecology== | |||
Habitat; symbiosis; contributions to the environment. | |||
==Pathology== | |||
<i>R. paucula</i> can cause clusters of nosocomial infections. Treatment of such infections was mainly based on the use of beta-lactams such as cefotaxime, ceftriaxone, piperacillin, and imipenem.[1] | |||
==Current Research== | |||
[ | ==References== | ||
<sup>[1]</sup>[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC87738/D Moissenet, D., Bidet, P., Garbarg-Chenon, A. Arlet, G., and Vu-Thien1, H. "Ralstonia paucula (Formerly CDC Group IV c-2): Unsuccessful Strain Differentiation with PCR-Based Methods, Study of the 16S-23S Spacer of the rRNA Operon, and Comparison with Other Ralstonia Species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum)". J Clin Microbiol. 2001 January; 39(1): 381–384.] | |||
Edited by Karolina Wala, Alma Shebib students of [mailto:mglogow@luc.edu M Glogowski]at [http://www.luc.edu/biology/index.html Loyola University] | |||
Edited by Karolina Wala, Alma Shebib | |||
<!-- Do not edit or remove this line-->[[Category:Pages edited by students of Mary Glogowski at Loyola University]] | |||
Revision as of 20:01, 3 May 2010
Ralstonia paucula
Classification
Higher order taxa
Domain; Phylum; Class; Order; family [Others may be used. Use NCBI link to find]
Species
|
NCBI: Taxonomy |
Ralstonia paucula
Description and significance
Ralstonia paucula is a Gram-negative environmental bacterium. Isolated from pool water, groundwater, bottled mineral water, and many clinical specimens. Ralstonia paucula, despite its low pathogenicity, is now recognized as an opportunist pathogen that can generate serious infections, such as septicemia, peritonitis, abscesses, etc., especially in immunocompromised patients.
16S Ribosomal RNA Gene Information
In 1995, bacteria was confronted with five cases of nosocomial bacteremia, we found that pulsed-field gel electrophoresis could not distinguish between the isolates and that randomly amplified polymorphic DNA analysis was poorly discriminatory. In this study, we used PCR-ribotyping and PCR-restriction fragment length polymorphism analysis of the spacer 16S-23S ribosomal DNA (rDNA); both methods were unable to differentiate R. paucula isolates. Eighteen strains belonging to other Ralstonia species (one R. eutropha strain, six R. pickettii strains, three R. solanacearum strains, and eight R. gilardii strains) were also tested by PCR-ribotyping, which failed to distinguish between the four species. The 16S-23S rDNA intergenic spacer of R. paucula contains the tRNAIle and tRNAAla genes, which are identical to genes described for R. pickettii and R. solanacearum. [1]
Cell structure and metabolism
Interesting features of cell structure; how it gains energy; what important molecules it produces.
Ecology
Habitat; symbiosis; contributions to the environment.
Pathology
R. paucula can cause clusters of nosocomial infections. Treatment of such infections was mainly based on the use of beta-lactams such as cefotaxime, ceftriaxone, piperacillin, and imipenem.[1]
Current Research
References
Edited by Karolina Wala, Alma Shebib students of M Glogowskiat Loyola University
