User:Melissa.winkler

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Revision as of 01:29, 4 May 2018 by Melissa.winkler (talk | contribs) (Physiology and Pathogenesis)
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Classification


Domain: Bacteria

Phylum: Actinobacteria

Class: Actinobacteria

Order: Actinomycetales

Family: Micrococcaceae

Genus: Arthrobacter

Species: protophormiae


Species

NCBI: Taxonomy [1]

Arthrobacter protophormiae

Habitat Information

The location in which the soil sample was obtained is in Austin, Texas, at the ACC Riverside campus, between two buildings. The exact GPS coordinates for this location are 30.237284, -97.704893. Collection was done around noon on January 26, 2018. The temperature was 57 degrees F with 81% humidity and 30.23 in of pressure. Winds were directed North and there was a slight overcast. It did not rain that day, However, it had rained five days prior. Soil was collected approximately two inches from the surface to eliminate as many rocks as possible.


Description and Significance

When cultured on an LB agar, Arthrobacter Protophormiae colonies were small yellow and raised. When testing the susceptibility to S. aureus and E.coli with the patch plate, our organism was only susceptible to S. aureus. Arthrobacter is commonly found in soil. Arthrobacteria are nutritionally versatile, using a variety of substrates in their oxidative metabolism including nicotine, nucleic acids, and various herbicides and pesticides. The cells are able to survive under stressful conditions induced by starvation, ionizing radiation, oxygen radicals, and toxic chemicals. Arthrobacter species have been isolated a few times from patients with immunodeficiencies but most strains do not appear to be pathogenic. A distinctive feature of this genus is that the shape of the cells change during the growth cycle, typically forming rods during early growth and cocci in the later stages. Arthrobacteria are nonsporulating and are a gram-positive bacteria.

Genome Structure

Our 16S ribosomal sequence we obtained from PCR and sequencing is:

FORWARD:

REVERSE: ACGACTCCCCCCACACAAGGTGGTTAGGCCATCGGCTTCGGGTGTTACCAACTTTCGTGAC TTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAGCGTTGCTGATCTGCGATTACTAGCGACTCCGACTTC ATGGGGTCGAGTTGCAGACCCCAATCCGAACTGAGACCGGCTTTTAGGGATTAGCTCCACCTCACAGTATCGCAACCCAT TGTACCGGCCATTGTAGCATGCGTGAAGCCCAAGACATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGAG TTGACCCCGGCAGTCTCCCATGAGTCCCCGGCATAACCCGCTGGCAACATGGAACGAGGGTTGCGCTCGTTGCGGGACTT AACCCAACATCTCACGACACGAGCTGACGACAACCATGCACCACCTGTGAACCAGCCCCGAAGGGAAACCCCATCTCTGA GGCGGTCTGGAACATGTCAAGCCTTGGTAAGGTTCTTCGCGTTGCATCGAATTAATCCGCATGCTCCGCCGCTTGTGCGG GCCCCCGTCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTACGGCGCGG AAAACGTGGAATGTCCCCCACACCTAGTGCCCAACGTTTACGGCATGGACTACCAGGGTATCTAATCCTGTTCGCTCCCC ATGCTTTCGCTCCTCAGCGTCAGTAAATGCCCAGAGACCTGCCTTCGCCATCGGTGTTCCTCCTGATATCTGCGCATTTC ACCGCTACACCAGGAATTCCAGTCTCCCCTACATCACTCTAGTCTGCCCGTACCCACCGCAGATCCGANGTTGAGCCTCG GACTTTCACGGCAGACGCGACAAACCGCCTACGAGCTCTTTACGCCCAATAAATCCGGATAACGCTTGCGCCCTACGTAT TACCGCG

Cell Structure, Metabolism and Life Cycle

Physiology and Pathogenesis

BIOCHEMICAL TEST RESULTS

Methyl Red Test for Arthrobacter oxydans
  • Phenol Red Broth Tests: Glucose: ; Lactose: ; Sucrose:
  • Starch Hydrolysis Test:
  • Casein Hydrolysis Test:
    Citrate Test for Arthrobacter oxydans
  • Gelatin Hydrolysis Test:
    SIM Test for Arthrobacter oxydans
  • DNA Hydrolysis Test:
    Nitrate Test for Arthrobacter oxydans
  • Lipid Hydrolysis Test:
    Urea Hydrolysis Test for Arthrobacter oxydans
  • Methyl Red Test:
    Triple Sugar Iron Test for Arthrobacter oxydans
  • Voges Proskauer Test:
  • Citrate Test:
  • SIM Tests:
  • Nitrate Reduction:
  • Urea Hydrolysis:
  • Triple Sugar Iron Agar:
  • Oxidase Test:
  • Eosin Methylene Blue Agar (EMB) Test:
  • Hektoen Enteric Agar (HE) Test:
  • MacConkey Agar Test:
  • Decarboxylation Tests: Arginine: ; Lysine: ; Orinithine:
  • Phenylalanine Deaminase Test:
  • Catalase Test:
  • Blood Agar Test:
  • Mannitol Salt Agar (MSA) Test: negative
  • Phenylethyl Alcohol Agar (PEA) Test: negative
  • Bacitracin/Optochin Susceptibility Test: Bacitracin: susceptible; Optochin: resistant
  • Antimicrobial Sensitivity (Kirby-Bauer Method) Test: Vancomyocin: susceptible; Sufisoxazole: susceptible; Linezolid: susceptible Bacitracin: susceptible; Oxacillin: resistant; Cefoxifin: susceptible

References

Authors

Page authored by Melissa Winkler and Samantha Limon, students of Prof. Kristine Hollingsworth at Austin Community College.