https://microbewiki.kenyon.edu/index.php?title=Vibrio_harveyi&feed=atom&action=historyVibrio harveyi - Revision history2024-03-28T21:01:35ZRevision history for this page on the wikiMediaWiki 1.39.6https://microbewiki.kenyon.edu/index.php?title=Vibrio_harveyi&diff=64910&oldid=prevBarichD at 15:41, 1 July 20112011-07-01T15:41:23Z<p></p>
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</table>BarichDhttps://microbewiki.kenyon.edu/index.php?title=Vibrio_harveyi&diff=54908&oldid=prevBarichD at 19:16, 19 August 20102010-08-19T19:16:51Z<p></p>
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</table>BarichDhttps://microbewiki.kenyon.edu/index.php?title=Vibrio_harveyi&diff=19424&oldid=prevGillenk: /* Ecology */2007-07-11T15:46:38Z<p><span dir="auto"><span class="autocomment">Ecology</span></span></p>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>The interspecial communication skills of ''Vibrio harveyi'' have major effects on the way the bacteria <del style="font-weight: bold; text-decoration: none;">interacts </del>with the environment, whether it is luminescing, becoming virulent, or excreting nucleases for defense. Even though ''V. harveyi'' does not form symbiotic relationships, there one well documented example of a relationship with another microorganism causing a phenomenon known as the Milky Sea effect. This occurs when large areas of the ocean are filled with luminescing "''V. harveyi'' living in association with colonies of the microalga ''Phaeocystis''." (Miller, 2005)</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>The interspecial communication skills of ''Vibrio harveyi'' have major effects on the way the bacteria <ins style="font-weight: bold; text-decoration: none;">interact </ins>with the environment, whether it is luminescing, becoming virulent, or excreting nucleases for defense. Even though ''V. harveyi'' does not form symbiotic relationships, there <ins style="font-weight: bold; text-decoration: none;">is </ins>one well documented example of a relationship with another microorganism causing a phenomenon known as the Milky Sea effect. This occurs when large areas of the ocean are filled with luminescing "''V. harveyi'' living in association with colonies of the microalga ''Phaeocystis''." (Miller, 2005)</div></td></tr>
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</table>Gillenkhttps://microbewiki.kenyon.edu/index.php?title=Vibrio_harveyi&diff=19423&oldid=prevGillenk: /* Description and significance */2007-07-11T15:45:07Z<p><span dir="auto"><span class="autocomment">Description and significance</span></span></p>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[Image:Vh1.JPG|thumb|Courtesy of Bonnie Bassler Lab]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[Image:Vh1.JPG|thumb|Courtesy of Bonnie Bassler Lab]]</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>''V. harveyi'' is a natural inhabitant of seawater, though it has been documented mainly in the tropics. Unlike its close relative [[''Vibrio fischeri'']] it is free-living and does not form symbiotic relationships, but is a common pathogen to many marine organisms. In applied science ''Vibrio <del style="font-weight: bold; text-decoration: none;">Harveyi</del>'' is an integral part of studying inter- and intra-bacterial communication, quorum sensing, as well as bioluminescence. Sequencing of the genome <del style="font-weight: bold; text-decoration: none;">would </del>undoubtedly contribute to further understanding of how bacteria sense the world around them and then make decisions based on their environment. ''V. harveyi'' is curved rod shaped, Gram-negative, and monotrichous (mobilized by a single polar flagellum).</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>''V. harveyi'' is a natural inhabitant of seawater, though it has been documented mainly in the tropics. Unlike its close relative [[''Vibrio fischeri'']] it is free-living and does not form symbiotic relationships, but is a common pathogen to many marine organisms. In applied science ''Vibrio <ins style="font-weight: bold; text-decoration: none;">harveyi</ins>'' is an integral part of studying inter- and intra-bacterial communication, quorum sensing, as well as bioluminescence. Sequencing of the genome <ins style="font-weight: bold; text-decoration: none;">will </ins>undoubtedly contribute to further understanding of how bacteria sense the world around them and then make decisions based on their environment. ''V. harveyi'' is curved rod shaped, Gram-negative, and monotrichous (mobilized by a single polar flagellum).</div></td></tr>
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</table>Gillenkhttps://microbewiki.kenyon.edu/index.php?title=Vibrio_harveyi&diff=16751&oldid=prevRckendal: /* Application to Biotechnology */2007-06-05T07:29:04Z<p><span dir="auto"><span class="autocomment">Application to Biotechnology</span></span></p>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>The interspecial autoinducer AI-2, produced by ''V. harveyi'' could be a significant molecule in biotechnology. Analysis of the autoinducer could lead to developement of synthetic inhibitors of the communication pathway. This interference has the possibility to disrupt virulence factors leading to novel antimicrobials.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>The interspecial autoinducer AI-2, produced by ''V. harveyi'' could be a significant molecule in biotechnology. Analysis of the autoinducer could lead to developement of synthetic inhibitors of the communication pathway. This interference has the possibility to disrupt virulence factors<ins style="font-weight: bold; text-decoration: none;">, thus </ins>leading to novel antimicrobials.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::"AI-2 itself could well function as a broad-spectrum antibiotic that disrupts quorum-sensing systems in a wide variety of Gram-negative and Gram-positive pathogens." (Federle, 2003)</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::"AI-2 itself could well function as a broad-spectrum antibiotic that disrupts quorum-sensing systems in a wide variety of Gram-negative and Gram-positive pathogens." (Federle, 2003)</div></td></tr>
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</table>Rckendalhttps://microbewiki.kenyon.edu/index.php?title=Vibrio_harveyi&diff=16747&oldid=prevRckendal: /* Cell structure and metabolism */2007-06-05T07:27:53Z<p><span dir="auto"><span class="autocomment">Cell structure and metabolism</span></span></p>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>''Vibrio harveyi'' is a facultative anaerobe, meaning it can swap between aerobic respiration of oxygen and fermentation in order to produce ATP. This characteristic helps ''V. harveyi'' survive in low oxygen concentrations, if fermentable material is present. ''V. harveyi'' has the ability of bioluminescence, which is controlled by the regulatory gene luxR as well as population density-sensing (quorum sensing) autoinducer signaling molecules. Expression occurs when the enzyme luciferace oxidizes an organic compound releasing free energy in the form of light. (Showalter, 1990)</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>''Vibrio harveyi'' is a facultative anaerobe, meaning it can swap between aerobic respiration of oxygen and fermentation in order to produce ATP. This characteristic helps ''V. harveyi'' survive in low oxygen concentrations, if fermentable material is present. ''V. harveyi'' has the ability of bioluminescence, which is controlled by the regulatory gene luxR as well as population density-sensing (quorum sensing) autoinducer signaling molecules. Expression occurs when the enzyme luciferace oxidizes an organic compound releasing free energy in the form of light. (Showalter, 1990)</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>''Vibrio harveyi'' is known to regulate gene expression based on its population density. This <del style="font-weight: bold; text-decoration: none;">ability </del>is known as "Quorum-Sensing". This cell-to-cell communication ability is due to the release of autoinducers as a function of population in a given area and can occur between different bacterial species. Some examples of regulated activities based on quorum sensing include virulence, antibiotic production, biofilm formation, symbiosis, motility, and luminescence. ''V. harveyi'' actually produces two different autoinducer signals; one for intraspecies communication (denoted AI-1) and one for interspecies communication (AI-2). (Henke, 2004)</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>''Vibrio harveyi'' is known to regulate gene expression based on its population density. This is known as "Quorum-Sensing". This cell-to-cell communication ability is due to the release of autoinducers as a function of population in a given area and can occur between different bacterial species. Some examples of regulated activities based on quorum sensing include virulence, antibiotic production, biofilm formation, symbiosis, motility, and luminescence. ''V. harveyi'' actually produces two different autoinducer signals; one for intraspecies communication (denoted AI-1) and one for interspecies communication (AI-2). (Henke, 2004)</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Ecology==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Ecology==</div></td></tr>
</table>Rckendalhttps://microbewiki.kenyon.edu/index.php?title=Vibrio_harveyi&diff=16746&oldid=prevRckendal: /* Cell structure and metabolism */2007-06-05T07:27:08Z<p><span dir="auto"><span class="autocomment">Cell structure and metabolism</span></span></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 07:27, 5 June 2007</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>''Vibrio harveyi'' is a facultative anaerobe, meaning it can swap between aerobic respiration of oxygen and fermentation in order to produce ATP. This characteristic helps ''V. harveyi'' survive in low oxygen concentrations, if fermentable material is present. ''V. harveyi'' has the ability of bioluminescence, which is controlled by the regulatory gene luxR as well as population density-sensing (quorum sensing) autoinducer signaling molecules. Expression occurs when the enzyme luciferace oxidizes an organic compound releasing free energy in the form of light. (Showalter, 1990)</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>''Vibrio harveyi'' is a facultative anaerobe, meaning it can swap between aerobic respiration of oxygen and fermentation in order to produce ATP. This characteristic helps ''V. harveyi'' survive in low oxygen concentrations, if fermentable material is present. ''V. harveyi'' has the ability of bioluminescence, which is controlled by the regulatory gene luxR as well as population density-sensing (quorum sensing) autoinducer signaling molecules. Expression occurs when the enzyme luciferace oxidizes an organic compound releasing free energy in the form of light. (Showalter, 1990)</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div> </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>''Vibrio harveyi'' is known to regulate gene expression based on its population density<ins style="font-weight: bold; text-decoration: none;">. This ability is known as "Quorum-Sensing"</ins>. This cell-to-cell communication ability is due to the release of autoinducers as a function of population in a given area and can occur between different bacterial species. Some examples of regulated activities based on quorum sensing include virulence, antibiotic production, biofilm formation, symbiosis, motility, and luminescence. ''V. harveyi'' actually produces two different autoinducer signals; one for intraspecies communication (denoted AI-1) and one for interspecies communication (AI-2). (Henke, 2004)</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">'''Quorum-Sensing:''' </del>''Vibrio harveyi'' is known to regulate gene expression based on its population density. This cell-to-cell communication ability is due to the release of autoinducers as a function of population in a given area and can occur between different bacterial species. Some examples of regulated activities based on quorum sensing include virulence, antibiotic production, biofilm formation, symbiosis, motility, and luminescence. ''V. harveyi'' actually produces two different autoinducer signals; one for intraspecies communication (denoted AI-1) and one for interspecies communication (AI-2). (Henke, 2004)</div></td><td colspan="2" class="diff-side-added"></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Ecology==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Ecology==</div></td></tr>
</table>Rckendalhttps://microbewiki.kenyon.edu/index.php?title=Vibrio_harveyi&diff=16742&oldid=prevRckendal: /* Cell structure and metabolism */2007-06-05T07:26:20Z<p><span dir="auto"><span class="autocomment">Cell structure and metabolism</span></span></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 07:26, 5 June 2007</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>''V. harveyi'' is Gram-negative, therefore it has a cell wall that consists of two membranes: an outer membrane made up of lipopolysaccharides and an inner cytoplasmic membrane. In between these is a periplasmic space housing a peptidoglycan layer.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>''V. harveyi'' is Gram-negative, therefore it has a cell wall that consists of two membranes: an outer membrane made up of lipopolysaccharides and an inner cytoplasmic membrane. In between these is a periplasmic space housing a peptidoglycan layer.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>''Vibrio harveyi'' is a facultative anaerobe, meaning it can swap between aerobic respiration of oxygen and fermentation in order to produce ATP. This characteristic helps ''V. harveyi'' survive in low oxygen concentrations, if fermentable material is present. ''V. harveyi has the ability of bioluminescence, which is controlled by the regulatory gene luxR as well as population density-sensing (quorum sensing) autoinducer signaling molecules. Expression occurs when the enzyme luciferace oxidizes an organic compound releasing free energy in the form of light. (Showalter, 1990)</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>''Vibrio harveyi'' is a facultative anaerobe, meaning it can swap between aerobic respiration of oxygen and fermentation in order to produce ATP. This characteristic helps ''V. harveyi'' survive in low oxygen concentrations, if fermentable material is present. ''V. harveyi<ins style="font-weight: bold; text-decoration: none;">'' </ins>has the ability of bioluminescence, which is controlled by the regulatory gene luxR as well as population density-sensing (quorum sensing) autoinducer signaling molecules. Expression occurs when the enzyme luciferace oxidizes an organic compound releasing free energy in the form of light. (Showalter, 1990)</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
</table>Rckendalhttps://microbewiki.kenyon.edu/index.php?title=Vibrio_harveyi&diff=16739&oldid=prevRckendal: /* Cell structure and metabolism */2007-06-05T07:25:38Z<p><span dir="auto"><span class="autocomment">Cell structure and metabolism</span></span></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">'''Structure:''' Because </del>''V. harveyi'' is Gram-negative it has a cell wall that consists of two membranes: an outer membrane <del style="font-weight: bold; text-decoration: none;">full </del>of lipopolysaccharides and an inner cytoplasmic membrane. In between these is a periplasmic space housing a peptidoglycan layer.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>''V. harveyi'' is Gram-negative<ins style="font-weight: bold; text-decoration: none;">, therefore </ins>it has a cell wall that consists of two membranes: an outer membrane <ins style="font-weight: bold; text-decoration: none;">made up </ins>of lipopolysaccharides and an inner cytoplasmic membrane. In between these is a periplasmic space housing a peptidoglycan layer.</div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">''Vibrio harveyi'' is a facultative anaerobe, meaning it can swap between aerobic respiration of oxygen and fermentation in order to produce ATP. This characteristic helps ''V. harveyi'' survive in low oxygen concentrations, if fermentable material is present. ''V. harveyi has the ability of bioluminescence, which is controlled by the regulatory gene luxR as well as population density-sensing (quorum sensing) autoinducer signaling molecules. Expression occurs when the enzyme luciferace oxidizes an organic compound releasing free energy in the form of light. (Showalter, 1990)</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">'''Metabolism:''' ''Vibrio harveyi'' is a heterotrophic, facultative anaerobic bacterium meaning it can swap between aerobic respiration and fermentation. This characteristic helps ''V. harveyi'' survive in low oxygen concentrations when enough fermentable material is present.</del></div></td><td colspan="2" class="diff-side-added"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">Bioluminescence is controlled by the regulatory gene luxR in ''V. harveyi'' as well as population density-sensing (quorum sensing) autoinducer signaling molecules. Expression occurs when the enzyme luciferace oxidizes an organic compound releasing free energy in the form of light!</del></div></td><td colspan="2" class="diff-side-added"></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div> </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>'''Quorum-Sensing:''' ''Vibrio harveyi'' is known to regulate gene expression based on its population density. This cell-to-cell communication ability is due to the release of autoinducers as a function of population in a given area and can occur between different bacterial species. Some examples of regulated activities based on quorum sensing include virulence, antibiotic production, biofilm formation, symbiosis, motility, and luminescence. ''V. harveyi'' actually produces two different autoinducer signals; one for intraspecies communication (denoted AI-1) and one for interspecies communication (AI-2). <ins style="font-weight: bold; text-decoration: none;">(Henke, 2004)</ins></div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>'''Quorum-Sensing:''' ''Vibrio harveyi'' is known to regulate gene expression based on its population density. This cell-to-cell communication ability is due to the release of autoinducers as a function of population in a given area and can occur between different bacterial species. Some examples of regulated activities based on quorum sensing include virulence, antibiotic production, biofilm formation, symbiosis, motility, and luminescence. ''V. harveyi'' actually produces two different autoinducer signals; one for intraspecies communication (denoted AI-1) and one for interspecies communication (AI-2).</div></td><td colspan="2" class="diff-side-added"></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Ecology==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Ecology==</div></td></tr>
</table>Rckendalhttps://microbewiki.kenyon.edu/index.php?title=Vibrio_harveyi&diff=16711&oldid=prevRckendal: /* Application to Biotechnology */2007-06-05T07:20:40Z<p><span dir="auto"><span class="autocomment">Application to Biotechnology</span></span></p>
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</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l59">Line 59:</td>
<td colspan="2" class="diff-lineno">Line 59:</td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The interspecial autoinducer AI-2, produced by ''V. harveyi'' could be a significant molecule in biotechnology. Analysis of the autoinducer could lead to developement of synthetic inhibitors of the communication pathway. This interference has the possibility to disrupt virulence factors leading to novel antimicrobials.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The interspecial autoinducer AI-2, produced by ''V. harveyi'' could be a significant molecule in biotechnology. Analysis of the autoinducer could lead to developement of synthetic inhibitors of the communication pathway. This interference has the possibility to disrupt virulence factors leading to novel antimicrobials.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::"AI-2 itself could well function as a broad-spectrum antibiotic that disrupts quorum-sensing systems in a wide variety of Gram-negative and Gram-positive pathogens." (<del style="font-weight: bold; text-decoration: none;">Bassler</del>, 2003)</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::"AI-2 itself could well function as a broad-spectrum antibiotic that disrupts quorum-sensing systems in a wide variety of Gram-negative and Gram-positive pathogens." (<ins style="font-weight: bold; text-decoration: none;">Federle</ins>, 2003)</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Current Research==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Current Research==</div></td></tr>
</table>Rckendal