Bluetongue Virus (BTV)

Bluetongue Virus (BTV)

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A Viral Biorealm page on the family Bluetongue Virus (BTV)

Clinical signs of bluetongue infection in ruminants such as sheep include swelling and hyperaemia of the mouth and lips. [1].

Baltimore Classification

Group III: Double-Stranded RNA Viruses

Higher order categories

Order: Unassigned
Family: Reoviridae
Subfamily: Sedoreovirinae
Genus: Orbivirus

Description and Significance

Bluetongue Virus (BTV) is a pathogenic virus that causes serious disease in livestock. BTV was first observed in Africa in the late 18th century, but has since been observed in Australia, the United States, Africa, the Middle East, Asia, and Europe. There are 24 distinct serotypes of BTV. BTV causes Bluetongue disease in ruminant species, such as sheep, goats, deer, and cattle, although clinical signs of infection are almost solely restricted to sheep [4].

The incubation period for BTV is 5-20 days. BTV infection first manifests itself with the onset of a high fever that may last several days. The virus mainly affects small blood vessels, causing hemorrhaging, hyperemia, and edema in the tissue of the lips, mouth, nasal linings, and eyelids. Swelling of the lips and tongue, accompanied by viral destruction of host blood vessels decreases the amount of oxygen that reaches the tongue tissue. Reduced oxygen delivery causes cyanosis, or the blue appearance of the tongue. However, cyanosis is usually confined to the minority of Bluetongue cases [5].

Swelling of the lips and tongue upon BTV infection can lead to frothing. [2].

The mortality rate of especially susceptible breeds of sheep can be as high as 70%, as a result of secondary bacterial infections. Furthermore, the high fever experienced by infected sheep can lead to wool breaks, decreasing productivity [2]. Some species, primarily cattle and goats, are asymptomatic upon BTV infection, but their productivity as livestock can be significantly reduced, with milk yields dropping nearly 40% [6]. BTV has been considered a possible bioweapon because of the difficulty associated with controlling and eliminating the disease, as well as the significant economic loss that would result from widespread infection [7].

There are no treatment options for BTV. Immunization has proved the most effective way to prevent bluetongue outbreaks in endemic regions. In southern Africa and other affected regions, a vaccine comprised of 5 attenuated BTV serotypes is widely used. In the United States, a vaccine containing a modified live version of a single serotype is utilized [8].

Genome Structure

The genome of BTV consists of a strand of linear dsRNA, 19,200 base pairs in length. The dsRNA genome can be split into 10 segments, each fragment encoding a single viral protein. The size of each segment ranges from 822 to 3954 base pairs in length. Fragment 10 is the only fragment which encodes two proteins, NS3 and NS3A, although both proteins are highly related and are the result of alternative initiation [9].

The dsRNA genome of BTV consists of 10 segments, encoding a total of 11 viral proteins. [3].

Protein-coding dsRNA fragments are translated by RNA-dependent RNA polymerase, which is encoded by VP1, segment 1 of the dsRNA genome [10]. Segments 2 and 6 produce VP2 and VP5, respectively, which form the outer layer of the capsid. Segment 7 encodes VP7, which forms the core-surface layer. Segment 3 produces VP3, the protein that forms the subcore shell. Segments 4 and 9 produce VP4 and VP6, respectively. VP4 forms an mRNA capping enzyme, while VP6 yields viral helicase. In conjunction with the polymerase produced by VP1, VP4 and VP6 form transcriptase complexes [11]. Segment 8 encodes NS2, which forms important viral inclusion body matrix proteins [12]. Viral proteins NS3 and NS3A form viral membrane proteins[13] and glycoproteins[14].

References

Example: Weir, Jerry P. " Genomic Organization and Evolution of the Human Herpesviruses." Virus Genes 16.1 (1998): 85-93.

Page authored for BIOL 375 Virology, September 2010