Pelagibacterales (SAR11)

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Classification

Domain: Bacteria

FIGURE 1. Scanning electron microscope image of SAR11 (1).

Phylum: Proteobacteria

Class: Alphaproteobacteria

Subclass: Rickettsidae

Order: Pelagibacterales

Description and Significance

Pelagibacterales, also known as the SAR11 clade, are the smallest and the most abundant marine organisms and as such are an important factor in carbon cycling. Bacteria of the SAR11 clade (named originally following their discovery in the Sargasso Sea) have the smallest genomes of any free-living microorganism. (2)(3)

SAR11 makes up a quarter of the marine microorganisms in the oceans’ surface layers on average and its population rises and falls with seasonal shifts from summer to winter just as levels of organic carbon in the water vary during the same cycle. The bacteria are a major consumer of organic carbon, which moves from the upper ocean layers where enough light penetrates to allow green plants to grow down to the deeper waters where it can be sequestered, and SAR11 produce the nutrients that allow algae to daily supply half the oxygen that becomes part the Earth’s atmosphere. Three SAR11 bacterial genomes have already been sequenced; sequencing several more contributes significant information about the marine carbon cycle. The work is relevant to the DOE’s mission to reduce and stabilize the levels of greenhouse gases in the atmosphere through methods such as carbon sequestration.

is a gram-positive, non-motile, chemoorganotrophic lactic acid bacterium that grows in chains of circular to ellipsoidal cells (2). Unlike many bacteria, it thrives in the harsh environment of wine and is considered beneficial microbe to certain styles of wine, typically adding to the wine's quality and character, rather an agent of damaging value (3). O. oeni is beneficial to wine and the entire field of oenology because of its primary ability to perform malolactic fermentation, the crucial secondary fermentation in the production of wine of converting malic acid to lactic acid. The end products of malolactic fermentation by O. oeni are responsible for the desired flavors that winemakers seek out. Though it occurs naturally in musts, wines, and fruit mashes, there are industrial standardized strains of O. oeni that are carefully cultured for large scale wine production (4).

In 1995, Dicks et al. sought to reclassify Leuconostoc oenos as Oenococcus oeni (Oeno- suffix, Greek for wine)because there is little DNA homology between the two genera (2). Oenococcus differs further from Leuconostoc in its abilities to use saccharose, lactose, and maltose as a substrate (5). In 2006, a second Oenococcus species was identified, Oenococcus kitaharae (6).

Genome Structure

An industrial standard strain of O. oeni, PSU-1, has a circular genome of 1780517 basepair nucleotides, 1691 protein-coding genes, and 51 RNA genes (7). 43 tRNA sequences that represent 20 amino acids and 14 different insertion sequence transposase genes are present. Comparing different strains of O. oeni genomes, the general number of nucleotides tends to be very similar across the specie (7). The critical malolactic fermentation operates from the mleA gene, coding for the malolactic enzyme that breaks down the malic acid in the environment. Morphological and genomic evidence established the grounds to reclassify this bacteria as Oenococcus oeni.

FIGURE 3. Genome of Oenococcus oeni PSU-1. Inner most circle shows ORF classification: (1) Information storage, (2) cellular processes, (3) metabolism, (4) poorly characterized, (5) uncharacterized. The next circle outwards shows ORF orientation. Next circle next shows tRNA (green) and rRNA (blue). The red dots are transposase genes. The green (low) and orange (high) bands show deviation in GC content. (8).

Cell Structure, Metabolism and Life Cycle

Oenococcus oeni is a facultative anaerobe. It is able to use oxygen for cellular respiration but can also gain energy through fermentation. It characteristically grows well in the environments of wine, being able to survive in acidic conditions below pH 3.0 and tolerant of ethanol levels above 10% (2). Optimal growth occurs on sugar and protein rich media, like grape or tomato juice. The cocci are ellipsoidal to spherical in shape, usually grow in chains or pairs, and are typically non-sporulating. Lactic acid bacteria, like Oenococcus oeni, perform malolactic fermentation (also known as malolatic conversion). It occurs after (or sometimes during) primary fermentation. The main function of malolatic fermentation is converting glucose and malic acid to lactic acid. This is occurs by the uptake of malate, decarboxylation of malate to L-lactic acid and carbon dioxide, and the export of end products. O. oeni is heterofermentative, meaning it can create multiple end products from fermenting the sugars. In O. oeni’s case, it produces carbon dioxide, ethanol, and acetate, as well as characteristic flavor molecules like diacetyl. Strain variation of Oenoccocus oeni cellular processes can have significant effects on the community dynamics, fermentation, and overall quality of wine. Strain variation exists in sugar utilization pathways, phosphototransferase enzyme II systems, bacteriophage integration, and cell wall exopolysaccharides (3).

Ecology

Oenococcus oeni stabilizes wine communities by consuming available nutrients and lowering potential growth of other microbes, but its malolactic fermentation can be beneficial or detrimental to the production of wine depending on grapes, climate, and style of wine. Variations between strains and fermentation conditions have the potential to impact general quality and production of wine. Industrial winemakers use a standardized strain of O. oeni, but the many external and environmental variables will dictate the success of the wine. O. oeni is not the only lactic acid bacteria that can perform secondary fermentation. There are a variety of lactic acid bacteria that can dominate bacterial community in response to temperature, nutrients, sulfur dioxide content, pH, ethanol levels, and inoculation densities. O. oeni commonly dominates secondary fermentation from its extreme tolerance to pH and ethanol levels. The molecule diacetyl is produced as a byproduct of lactic acid bacteria in secondary fermentation. In wine, the levels of diacetyl create buttery and caramel flavor notes. It is generated when there is little or no malic acid to be consumed so citric acid is used. This byproduct is sought out by some winemakers while it is avoided by others. Rarely do the diacetyl levels reach a point of spoiling the wine (10).

Because of its heterofermentive properties, Oenoccocus may be viewed as an ecosystem engineer. O. oeni plays a major role in establishing the environment for which other microbes will interact. Its end products and life strategies positively feedback into creating a more harsh environment for other microbes like yeasts and fungi while making the conditions more ideal for other lactic acid bacteria (5).

References

(1) "Rebounding bacteria". 2013

(2) Dicks, L. M. T., Dellaglio, F., Collins, M. D. “Proposal To Reclassify Leuconostoc oenos as Oenococcus oeni corrig. gen. nov., comb. nov”.“International Journal of Systematic Bacteriology”. 1995. Volume 445. P. 395-397

(3) Borneman, A. R., McCarthy, J. M., Chambers, P. J., Bartowsky, E. J. “Comparative analysis of the Oenococcus oeni pan genome reveals genetic diversity in industrially-relevant pathways”. 2012. BMC Genomics. Volume 13. P. 373

(4) Oenococcus oeni PSU-1. The Regents of the University of California

(5) Solieri, L., Genova, F., De Palola, M., Giudici, P. “Characterization and technological properties of Oenococcus oeni strains from wine spontaneous malolactic fermentations: a framework for selection of new starter cultures”. “Journal of Applied Microbiology”. 2010. Volume 108. P. 285-298

(6) Endo, A., Okada, S. “Oenococcus kitaharae sp. nov., a non-acidophilic and non-malolactic-fermenting oenococcus isolated from a composting distilled shochu residue”. “International Journal of Systematic and Evolutionary Microbiology”. 2006. Volume 56. P. 2345-2348

(7) Kakarova, K., et al. “Comparative genomics of the lactic acid bacteria” . “Proc. Natl. Acad. Sci. USA.”. 2006. Volume 103. P. 15611-6

(8) Mills, D. A., Rawsthorne, H., Parker, C., Tamir, D., Makarova, K. “Genomic analysis of Oenococcus oeni PSU-1 and its relevance to winemaking”. “FEMS Microbiology Reviews”. 2005. Volume 29. P. 465-475

(9) phys.org “Raise your glass to Oenococcus oeni, a real wine bug”. “Science X Network”. 2014.

(10) “Gibson, G., Farkas, M. “Flaws and Faults in wine”. ”British Columbia Amateur Winemakers Association”. Accessed 28 April 2014

Author

Page authored by Digvinder Singh Mavi student of Prof. Katherine Mcmahon at University of Wisconsin-Madison.