User:Bca2592: Difference between revisions
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==Genome Structure== | ==Genome Structure== | ||
Similar to other microorganisms, the genome of Candidatus Ethanoperedens thermophilum is a single, circular chromosome. In order to understand the genomic makeup of the GoM-Arc1 genome, cultures were performed in both 37 degrees celcius and 50 degrees celcius resulting in a 76.2% genome comompletedness and 100% complete genetic sequence, respectivey. Due to their 98% similarity, both samples were able to be fully sequenced after long-read DNA sequenceing. | |||
The total length of the genome was found to be 1.25Mb with a protein count of 1,274 and a gene count of 1,329. There is also a GC content of 41.3%. When comparing metagenome-assembled genomes (MAGs) and 16S rRNA of this novel organism to others of the GoM-Arc1 clade derived from the Guaymas Basin and the Gulf of Mexico it is apparent that they have similar gene contents (over 90% average nucleotide identity (ANI) and 99.5% 16S rRNA gene identity) - ultimately suggesting that these GoM-Arc1 archaea are ethane oxidizers. | |||
An interesting fact about this organism is that it has the ability to reverse its metabolism of ethane to CO2 to CO2 to ethane which illuminates potential for future experiments regarding decreasing atmospheric carbon emmisions in industry. | |||
==Cell Structure, Metabolism and Life Cycle== | ==Cell Structure, Metabolism and Life Cycle== | ||
Revision as of 17:04, 14 November 2022
Classification
Archaea; Euryarchaeota; Stenosarchaea; Methanomicrobia; Methanosarcinales; Methanosarcinales incertae sedis; GOM Arc I cluster
Species
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NCBI: [1] |
Candidatus Ethanoperedens thermophilum
Description and Significance
Candidatus Ethanoperedens thermophilum is a thermophilic genus of archaea known to mediate the anaerobic oxidation of ethane in cold seeps and hydrothermal vents. Although previously challenging to study due to the inherently slow growth of this organism, this archaeon has recently been cultured using hydrothermal sediments of the Guaymas Basin (Gulf of California) with ethane as the substrate in order to further understand the mechanisms of archaeal alkane degradation (Hahn et al., 2020).
Describe the appearance, habitat, etc. of the organism, and why you think it is important.
Genome Structure
Similar to other microorganisms, the genome of Candidatus Ethanoperedens thermophilum is a single, circular chromosome. In order to understand the genomic makeup of the GoM-Arc1 genome, cultures were performed in both 37 degrees celcius and 50 degrees celcius resulting in a 76.2% genome comompletedness and 100% complete genetic sequence, respectivey. Due to their 98% similarity, both samples were able to be fully sequenced after long-read DNA sequenceing.
The total length of the genome was found to be 1.25Mb with a protein count of 1,274 and a gene count of 1,329. There is also a GC content of 41.3%. When comparing metagenome-assembled genomes (MAGs) and 16S rRNA of this novel organism to others of the GoM-Arc1 clade derived from the Guaymas Basin and the Gulf of Mexico it is apparent that they have similar gene contents (over 90% average nucleotide identity (ANI) and 99.5% 16S rRNA gene identity) - ultimately suggesting that these GoM-Arc1 archaea are ethane oxidizers.
An interesting fact about this organism is that it has the ability to reverse its metabolism of ethane to CO2 to CO2 to ethane which illuminates potential for future experiments regarding decreasing atmospheric carbon emmisions in industry.
Cell Structure, Metabolism and Life Cycle
Interesting features of cell structure; how it gains energy; what important molecules it produces.
Ecology and Pathogenesis
Habitat; symbiosis; biogeochemical significance; contributions to environment.
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.
References
Hahn CJ, Laso-Pérez R, Vulcano F, Vaziourakis KM, Stokke R, Steen IH, Teske A, Boetius A, Liebeke M, Amann R, Knittel K, Wegener G. "Candidatus Ethanoperedens," a Thermophilic Genus of Archaea Mediating the Anaerobic Oxidation of Ethane. mBio. 2020 Apr 21;11(2):e00600-20. doi: 10.1128/mBio.00600-20. PMID: 32317322; PMCID: PMC7175092.
Author
Page authored by _____, student of Prof. Bradley Tolar at UNC Wilmington.
