Endozoicomonas ascidiicola: Difference between revisions
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Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Oceanospirillales; Endozoicomonadaceae [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1698521 | Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Oceanospirillales; Endozoicomonadaceae [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1698521] | ||
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==Description and Significance== | ==Description and Significance== | ||
E. ascidiicola is represented by the type strain AVMART05T and strain KASP37. | |||
It is a Gram-negative (KOH positive), motile, rod-shaped bacterium, measuring 0.3–0.7um in diameter and 1.2–11.3um long. Colonies can be beige, circular and convex with a smooth surface and entire margins (strain AVMART05T) or transparent-white, circular and flat with a smooth surface and undulate margins (strain KASP37). Colonies measure 0.5–1 mm (AVMART05T) or 2–3 mm (KASP37) in diameter on marine agar after a 72 h incubation at 25◦C. | |||
E. ascidiicola is mesophilic, neutrophilic, and slightly halophilic, with optimal growth occurring at 23–25◦C, pH 6–7, and 1–2% NaCl. Cells are facultative anaerobic and reduce nitrate. | |||
Describe the appearance, habitat, etc. of the organism, and why you think it is important. | Describe the appearance, habitat, etc. of the organism, and why you think it is important. | ||
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Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? | Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? | ||
E. ascidiicola has a singular, circular genome. The genome contains at least three distinct 16S rRNA gene paralogs. | |||
The major cellular fatty acids are C18:1 7c, C16:1 7c, and C16:0. The only detected respiratory quinone is Q-9. The polar lipids comprise phosphatidylglycerol (PG), phosphatidylethanolamin (PE), and one phosphoaminolipid | The major cellular fatty acids are C18:1 7c, C16:1 7c, and C16:0. The only detected respiratory quinone is Q-9. The polar lipids comprise phosphatidylglycerol (PG), phosphatidylethanolamin (PE), and one phosphoaminolipid. | ||
Most prominent sites of sequence divergence are located in the V2 and V3 region of the 16S rRNA genes. | Most prominent sites of sequence divergence are located in the V2 and V3 region of the 16S rRNA genes. | ||
The DNA G + C content is | The DNA G+C content is ~47 mol%. | ||
==Cell Structure, Metabolism and Life Cycle== | ==Cell Structure, Metabolism and Life Cycle== | ||
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The peptidoglycan type is A1. | The peptidoglycan type is A1. | ||
Grows well on full and half-strength marine agar and marine broth. Grows well on porcine mucin agar | Grows well on full and half-strength marine agar and marine broth. Grows well on porcine mucin agar | ||
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==References== | ==References== | ||
[Schreiber L, Kjeldsen KU, Obst M, Funch P, Schramm A. 2016. Description of Endozoicomonas ascidiicola sp. nov., isolated from Scandinavian ascidians. Systematic and Applied Microbiology 39:313–318.] | |||
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Revision as of 00:05, 20 November 2024
Classification
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Oceanospirillales; Endozoicomonadaceae [1]
Species
|
NCBI: [2] |
Genus species
Description and Significance
E. ascidiicola is represented by the type strain AVMART05T and strain KASP37. It is a Gram-negative (KOH positive), motile, rod-shaped bacterium, measuring 0.3–0.7um in diameter and 1.2–11.3um long. Colonies can be beige, circular and convex with a smooth surface and entire margins (strain AVMART05T) or transparent-white, circular and flat with a smooth surface and undulate margins (strain KASP37). Colonies measure 0.5–1 mm (AVMART05T) or 2–3 mm (KASP37) in diameter on marine agar after a 72 h incubation at 25◦C.
E. ascidiicola is mesophilic, neutrophilic, and slightly halophilic, with optimal growth occurring at 23–25◦C, pH 6–7, and 1–2% NaCl. Cells are facultative anaerobic and reduce nitrate.
Describe the appearance, habitat, etc. of the organism, and why you think it is important.
Genome Structure
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence?
E. ascidiicola has a singular, circular genome. The genome contains at least three distinct 16S rRNA gene paralogs. The major cellular fatty acids are C18:1 7c, C16:1 7c, and C16:0. The only detected respiratory quinone is Q-9. The polar lipids comprise phosphatidylglycerol (PG), phosphatidylethanolamin (PE), and one phosphoaminolipid. Most prominent sites of sequence divergence are located in the V2 and V3 region of the 16S rRNA genes. The DNA G+C content is ~47 mol%.
Cell Structure, Metabolism and Life Cycle
Interesting features of cell structure; how it gains energy; what important molecules it produces.
The peptidoglycan type is A1. Grows well on full and half-strength marine agar and marine broth. Grows well on porcine mucin agar
Ecology and Pathogenesis
Ascidiicola = Ascidian-dweller
Symbiosis with invertebrates, including ascidians. It was first isolated from the pharynx tissue of the ascidian genus Ascidiella collected from Gullmarsfjord, Sweden in 2010.Habitat; symbiosis; biogeochemical significance; contributions to environment.
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.
References
[Schreiber L, Kjeldsen KU, Obst M, Funch P, Schramm A. 2016. Description of Endozoicomonas ascidiicola sp. nov., isolated from Scandinavian ascidians. Systematic and Applied Microbiology 39:313–318.]
Author
Page authored by Grace Kennedy, student of Prof. Bradley Tolar at UNC Wilmington.
