Staphylococcus aureus: Difference between revisions
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==Pathology== | ==Pathology== | ||
''Staphylococcus aureus'' is the most common cause of staph infections and is responsible for various diseases including: mild skin infections (impetigo, folliculitis, etc.), invasive diseases (wound infections, osteomyelitis, bacteremia with metastatic complications, etc.), and toxin mediated diseases (food poisoning, toxic shock syndrome or TSS, scaled skin syndrome, etc.). | |||
Infections are preceded by colonization. Common superficial infections include carbuncles, impetigo, cellulitis, folliculitis. Community-acquired infections include bacteremia, endocarditis, osteomylitis, pneumonia and wound infections are less common. S. aureus also causes economically important mastitis in cows, sheep and goats. | |||
In the late 1970's an epidemic of toxic shock syndrome (TSS T-1) was brought about by a change in host environment. The environment change was encouraged by a creation of modern technology otherwise known as the super-absorbent tampon. This new modern convenience product that had swept the nation's females actually created a new nutrient-rich surface area for many bacteria to thrive off of, ''S. aureus'' being a major resident. However, TSS from tampons can be easily avoided by correctly using tampons (read instructions and warning labels listed with tampon products). | |||
MRSA: | |||
The first serious emergence of antibiotic resistance staph occurred with a specific strain we refer to as Methicillin-Resistant Staphylococcus Aureus, abbreviated as MRSA. This strain expressed a modified penicillin-binding protein encoded by mecA gene and is present in 4 forms of Staph. Cassette Chromosome. The MRSA, resistant to the antibiotic methicillin, and eventually was isolated. Consequently, vancomycin (the most powerful antibiotic in our arsenal) became the primary antibiotic used to combat staphylococcus infection. In 1997 a strain of ''S. aureus'' resistant to vancomycin was isolated, and people are once again exposed to the threat of untreatable staphylococcus infection. MRSA strains are currently a very significant healthcare problem. The sequencing of the ''S. aureus'' genome will hopefully provide insight into how the organism generates such a variety of toxins, and aid researchers in developing ways of combating the versatile bacterium. | |||
==Application to Biotechnology== | ==Application to Biotechnology== | ||
Revision as of 18:36, 5 June 2007
A Microbial Biorealm page on the genus Staphylococcus aureus
Classification
Higher order taxa
Domain: Bacteria Kingdom: Bacteria Phylum: Firmicutes Class: Cocci Order: Bacillales Family: Staphylococcaceae Genus: Staphylococcus Species: Staphylococcus aureus
Species
Staphylococcus aureus
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NCBI: Taxonomy |
Description and significance
Staphylococci are spherical gram-positive bacteria, which are immobile and form grape-like clusters. They form bunches because they divide in two planes as opposed to their close relatives streptococci which form chains because they divide only in one plane. Colonies formed by S. aureus are yellow (thus the name aureus, Latin for gold) and grow large on a rich medium. Staphylococcus aureus and their genus Staphylococci are facultative anaerobes which means they grow by aerobic respiration or fermentation that produces lactic acid.
As a pathogen, it is important to understand the virulence mechanisms of S. aureus especially the Methicillin-resistant Staphylococcus aureus (MRSA) in order to successfully combat the pathogen. The increasing population of "super germs" and antibiotic resistant pathogens have increased pressure on researchers to find alternative, more effective ways of fighting these "super germs." DNA sequencing of this microbe has already isolated the source code of its' resistance to antibiotics, and further research will more than likely lead us to the path of our next artillery against this and many other pathogens.
Genome structure
The ‘‘Staphylococcus aureus’’ genome, which is the most common species among the Staphylococcus genome projects, is the most completed genome sequences compared to any other microbial species. The original genome map of ‘‘Staphylococcus aureus’’ was based on the strain NCTC 8325, initiated by Peter A. Pattee and colleagues. By 2000, the entire genome of strain 8325 had been sequenced and annotated. Since then, at least six other ‘‘S. aureus’’ strains have been completed (COL, N315, Mu50, MW2, MRSA252, MSSA476).
The ‘‘Staphylococcus aureus’’ strain NCTC 8325 complete circular genome map shows ~2,900 open reading frames, 61 tRNA genes, 3 structural RNAs, and 5 complete ribosomal RNA operons. This strain has about 33% G+C content and an average gene length of 824 nucleotides with 85% coding sequence, similar to other ‘‘S. aureus’’ strains. Half the coding sequence is located predominantly on one replichore and the second half is located predominantly on the other replichore.
Virulence factors are encoded by phages, plasmids, pathogenicy islands and staphylococcus cassette chromosome. Increased resistance for antibiotics is encoded by a transposon (Tn 1546) that was inserted into a conjugated plasmid that also encoded resistance to other things including disinfectants. MRSA (Methicillin-Resistant ‘‘Staphylococcus aureus’’), which is resistant to the antibiotic methicillin, expresses a modified penicillin-binding protein encoded by mecA gene. This was brought about by many evolutions thought horizontal gene transfer of mecA to a wide variety of methicillin susceptible ‘‘S. aureus’’ strains. The genes for antibiotic resistance in Staphylococcus aureus are located on plasmids or other similar structures.
Diversification within the ‘‘S. aureus’’ population is achieved through a combination of mutation, recombination and horizontal gene transfer. Evolution of this bacterium can occur through asymptomatic colonization and/or during the course of the caused disease.
Cell structure and metabolism
Staphylococcus aureus is a gram positive bacteria, which means that the cell wall of this bacteria consists of a very thick peptidoglycan layer. They form spherical colonies in clusters in 2 planes and have no flagella.
Secretions are numerous, but include surface associated adhesins, endotoxins, exoenzymes, capsular polysaccharide. The capsule is responsible for enhanced virulence of a mucoid strain.
The central routes of glucose metabolism are the Embden-Meyerhof-Parnas (EMP) pathway and the pentose phosphate cycle. Lactate is the end product of anaerobic glucose metabotlism and acetate and CO2 are the products of aerobic growth conditions. S. aureus can uptake a variety of nutrients including glucose, mannose, mannitol, glucosamine, N-acetylglucosamine, sucrose, lactose, glactose and beta-glucosides.
Ecology
Staphylococcus aureus is among the most common hospital acquired pathogens. It is normal inhabitant of the skin and mucous membranes in the nose of a healthy human. S. aureus is infectious to both animals and humans and may only survive on dry skin. It can be spread through contaminated surfaces, through the air and through people. Approximately 30% of the normal healthy population is affected by S. aureus as it asymptomatically colonizes on the skin of human hosts. Though some host colonization can be benign, a puncture or break in the skin can prompt this bacterium to enter a wound and cause infections. The best preventive measure is simply regular hand washing (preferably without antibacterial soaps or hand sanitizers, but that’s another story) and daily bathing
Pathology
Staphylococcus aureus is the most common cause of staph infections and is responsible for various diseases including: mild skin infections (impetigo, folliculitis, etc.), invasive diseases (wound infections, osteomyelitis, bacteremia with metastatic complications, etc.), and toxin mediated diseases (food poisoning, toxic shock syndrome or TSS, scaled skin syndrome, etc.). Infections are preceded by colonization. Common superficial infections include carbuncles, impetigo, cellulitis, folliculitis. Community-acquired infections include bacteremia, endocarditis, osteomylitis, pneumonia and wound infections are less common. S. aureus also causes economically important mastitis in cows, sheep and goats.
In the late 1970's an epidemic of toxic shock syndrome (TSS T-1) was brought about by a change in host environment. The environment change was encouraged by a creation of modern technology otherwise known as the super-absorbent tampon. This new modern convenience product that had swept the nation's females actually created a new nutrient-rich surface area for many bacteria to thrive off of, S. aureus being a major resident. However, TSS from tampons can be easily avoided by correctly using tampons (read instructions and warning labels listed with tampon products).
MRSA: The first serious emergence of antibiotic resistance staph occurred with a specific strain we refer to as Methicillin-Resistant Staphylococcus Aureus, abbreviated as MRSA. This strain expressed a modified penicillin-binding protein encoded by mecA gene and is present in 4 forms of Staph. Cassette Chromosome. The MRSA, resistant to the antibiotic methicillin, and eventually was isolated. Consequently, vancomycin (the most powerful antibiotic in our arsenal) became the primary antibiotic used to combat staphylococcus infection. In 1997 a strain of S. aureus resistant to vancomycin was isolated, and people are once again exposed to the threat of untreatable staphylococcus infection. MRSA strains are currently a very significant healthcare problem. The sequencing of the S. aureus genome will hopefully provide insight into how the organism generates such a variety of toxins, and aid researchers in developing ways of combating the versatile bacterium.
Application to Biotechnology
Does this organism produce any useful compounds or enzymes? What are they and how are they used?
Current Research
Enter summaries of the most recent research here--at least three required
References
Edited by student of Rachel Larsen and Kit Pogliano
