Sulfolobus solfataricus

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Classification

Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae

Species

NCBI: Taxonomy

Sulfolobus solfataricus

Microscopy image of S. solfataricus. Image courtesy of D.Janckovik and W.Zillig

Description and Significance

Sulfolobus solfataricus grows in volcanic hot springs where they have ample sulfur and low pH. S. solfataricus is a very widely studied crenarchaeal organism. It is used as a model organism in archaeal research, including DNA replication, the cell cycle, chromosomal integration, and RNA processing.

Proteins found in S. solfataricus are being investigated for use in biotechnology because they remain functional in a wide range of temperatures. S. solfataricus has also been used on the US Space Shuttle for protein crystallography studies.

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Genome Structure

Chromosome illustration of S. solfataricus showing gene function and location. Image courtesy of Qunxin She et al. (2001).

The genome of S. solfataricus P2 was sequenced in 2001. It consists of a single circular chromosome containing 2,992,245 base pairs. There are 3,032 genes which encode 2,977 proteins. It has been shown that approximately one-third of the S. solfataricus P2 genome is unique. However, much of the genome (≈40%) is archaeal, while small portions overlap with Bacteria and Eukarya (12 and 2.3%, respectively). It has its own 743 open reading frames, and about 11% of the genome is made up of mobile elements.

The S. solfataricus P2 genome has already been studied extensively. It has been integral in the study of crenarchaeal biology, and has been compared to many euryarchaea to elucidate differences between the two groups. It has also provided more evidence for the separations of archaea, eukarya, and bacteria. The S. solfataricus P2 genome has been manipulated for studies of transcription, translation, the cell cycle, and other genetic events. Currently, the Sulfolobus solfataricus 98/2 strain is being sequenced for further studies.

Cell Structure, Metabolism and Life Cycle

Sulfolobus solfataricus has a spherical shape and a clockwise flagellum. Its outer wall is unusual in that it does not contain any peptidoglycan. The cell wall contains: proteins that mediate the transport of essential carbohydrates into the cell; ion channels that keep potassium levels at vital concentrations; and ATPases that uptake protons for oxidative phosphorylation.

Metabolic pathways of S. solfataricus. Image courtesy of Qunxin She et al.

S. solfataricus can use one of two methods to get energy for growth. It can either oxidize sulfur, or it can metabolize various carbohydrates with the help of sulfur.

When S. solfataricus utilizes the glycolitic pathway, it converts glucose to pyruvate, which is used in the citric acid cycle. Glucose is not always readily available in the environment, and so S. solfataricus converts the carbohydrates present into glucose at an expense of energy. Glycolysis yields no net energy; however, the citric acid cycle produces some ATP and reduces NADP+ to NADPH that it can be used to drive oxidative phosphorylation. S. solfataricus also utilizes the pentose phosphate pathway when as a method of obtaining NADPH from carbohydrates that do not go through the glycolitic pathway.

S. solfataricus is able to synthesize all 20 amino acids.

S. solfataricus requires oxygen as its final electron acceptor. When necessary, S. solfataricus can utilize sulfide and hydrogen as electron donors.

Ecology and Pathogenesis

The red of these rocks found near Naples, Italy is created by S. solfataricus colonization. Image courtesy of Science@NASA (1998).

Sulfolobus solfataricus is a thermoacidophile, and was first characterized in sulfur-rich volcanic springs in Yellowstone National Park. Strains of S. solfataricus have also been found in hot mud pools in the Solfatara crater north of Naples, Italy. It has also been discovered in El Salvador and the Dominica. While the optimum temperature for growth is ≈75^oC, S. solfataricus can live in a range of 55-90^oC. It can live in a pH range of 0.9-5.8, with its optimum being 2-3. However, S. solfataricus maintains its cytoplasmic pH at 6.5. It has also been shown that S. solfataricus requires an aerobic environment, in which it exists as a free-living chemolithotroph.

Fusellovirus SSV1. Courtesy of Prangishvili, Stedman, Zillig (2001). Scale bar = 200nm

Some studies have shown that S. solfataricus auto-aggregate when exposed to UV light, creating multi-cellular structures. Extracellular type-IV pili-like structures have been observed due to the UV-induced increase in transcription of the pili-like forming genes. The formation of S. solfataricus aggregates has been shown to be independent of pH and temperature. In addition, strains of S. solfataricus are known to harbor plasmids that encourage bacteria-like conjugation between cells. However, the genes behind these mechanisms are dissimilar between S. solfataricus and bacteria.

S. solfataricus can be infected by morphologically unusual viruses, such as SSV1 (Sulfolobus spindle-shaped virus). The virions formed by SSV1 often cluster together, forming rosette-like shapes. These viruses have been found in Yellowstone National Park, Iceland, and Japan.

References

Baumann, H., Knapp, S., Ladenstein, R., and Hard, T. "Solution structure and DNA-binding properties from the archaeon Sulfolobus sofataricus". Nature: Structural Biology. 1994. Volume 1. Number 11.

Brock, T., Brock, K., Belly, R., and Weiss, R. "Sulfolobus: A new genus of sulfur-oxidizing bacteria living at low pH and high temperature". Archives of Microbiology. 1972. Volume 84. p. 54-68.

De Rosa, M., Gambacorta, A., Nicola, B., Giardina, P., Poerio, E., and Buonocore, V. "Glucose metabolism in the extremem thermoacidophilic archaebacterium Sulfolobus solfataricus". Journal of Biochemistry. 1984. Volume 224. p. 407-414.

Elferink, M.G.L., Schleper, C., and Zillig, W. "Transformation of the extremely therrnoacidophilic archaeon Sulfolobus solfataricus via a self-spreading vector". FEMS Microbiology Letters. 1996. Volume 137. p. 31-35.

Esposito, L. et al.. "Protein Crystal Growth in the Advanced Protein Crystallization Facility on the LMS Mission: a Comparison of Sulfolobus solfataricus Alcohol Dehydrogenase Crystals Grown on the Ground and in Microgravity". Biological Crystallography. 1998. Volume 54. p. 386-390

Frols, S. et al. "UV-inducible cellular aggregation of the hyperthermophilic archaeon Sulfolobus solfataricus is mediated by pili formation". Molecular Microbiology. 2008. Volume 70. p. 938-952.

Hennig, M., Darimont, B., Sterner, R., Kirschner, K., and Jansonious, J.N. "2.0 A structure of indole-3-glycerol phosphate synthase from the hyperthermophile Sulfolobus solfataricus: possible determinants of protein stability". Structure. 1995. Volume 3. p. 1295-1306.

Prangishvilli, D., Stedman, K., and Zillig, W. "Viruses of the extremely thermophilic archaeon Sulfolobus". Trends in Microbiology. 2001. Volume 9. Number 1.

She, Q., Singh, S., Confalonerl, F., et al. "The complete genome of the crenarchaeon Sulfolobus solfataricus P2". Proceedings of the National Academy of Sciences of the United States of America. 2001. Volume 98. p. 7835-7840.

Author

Page authored by Alvin Makohon-Moore and Antoni Malachowski, students of Prof. Jay Lennon at Michigan State University.