Bacillus Unknown: Difference between revisions

From MicrobeWiki, the student-edited microbiology resource
 
(45 intermediate revisions by the same user not shown)
Line 2: Line 2:
==Classification==
==Classification==


Domain; Phylum; Class; Order; family [Others may be used.  Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]
'''Domain''' : Bacteria <br>
 
'''Division/phylum''': Firmicutes <br>
'''Class''': Bacilli <br>
'''Order''': Bacillales <br>
'''Family''': Bacillaceae <br>
'''Genus''': Bacillus <br>
'''Species''': B. safensis <br>
===Species===
===Species===


Line 10: Line 15:
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''
|}
|}


''Genus species''
''Genus species''
* Terrabacteria Group
'' Molecule Type''
*Nucleic Acid


==Habitat Information ==
==Habitat Information ==
Describe the location and conditions under which the organism was isolated.


Types of Soil Organism was Found In:
 
* Heiden Clay, 5-8% slopes, eroded (most common)
*'''Types of Soil Organism was Found In:'''
* Houston Black Clay, 1-3% slopes
  Heiden Clay, 5-8% slopes, eroded (most common)
  Houston Black Clay, 1-3% slopes


[[Air Temp:]] 83 Degrees Fahrenheit/ 28 Degrees Celsius
[[Air Temp:]] 83 Degrees Fahrenheit/ 28 Degrees Celsius
Line 27: Line 37:
[[Wind at 4am:]] 2.06mph
[[Wind at 4am:]] 2.06mph
[[Wind at 4pm:]] 2.77mph
[[Wind at 4pm:]] 2.77mph
*'''Soil Location Description:'''
Soil was taken from directly under a tree at Cabana Beach Apartments in San Marcos, Texas. This area is a very open area and gets water daily from sprinklers. The water it gets daily is minimal, so it does dry quickly. The soil was a very dark brown color. Had almost a dry dirt look and feel to it.
[[File:SoilMap.jpg]]


==Description and Significance==
==Description and Significance==
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.
 
*'''Description:'''
Whiteish color, shiny, raised and semi flat
[[File:IMG_3267.jpg]]  [[File:IMG_3417.jpg]]
 
'''Most common similar Bacteria: Bacillus Safensis:'''
Bacillus Safensis is a Gram-positive, spore-forming rod bacterium. Bacillus safensis is also an aerobic, chemoheterotroph. Cell size ranges from 0.5-0.7 μm in diameter and 1.0-1.2 μm in length. Bacteria are motile, and use polar flagella for locomotion. Cells are considered mesophillic, as they can grow in temperatures ranging between 10-50 °C
 
It was first discovered in California and Florida on spacecraft and so is believed to have been brought to the USA from Mars. There are numerous strains of this bacterium, everyone belonging to the Firmicutes phylum of Bacteria. This organism is significant because it can tell us a bit about other planets and also how the bacteria spreads. It is also resistant to UV rays, gamma rays and is highly salt resistant, therefore it makes for a great plant growth-promoting rhizobacteria.


==Genome Structure==
==Genome Structure==
Describe the size and content of the genome.  How many chromosomes?  Circular or linear?  Other interesting features?  What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.




==Cell Structure, Metabolism and Life Cycle==
'''DNA Sequencing Results:'''
Interesting features of cell structure; how it gains energy; what important molecules it produces.
""CCACCTGTCACTCTGTCCCCGAAGGGAAAGCCCTATCTCTAGGGTTGTCAGAGGATGTCAAGACCTGGT
AAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAGT
CTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCAGCACTAAGGGGCGGAAACCCCCTAACACTTAGCA
CTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACAG
ACCAGAGAGTCGCCTTCGCCACTGGTGTTCCTCCACATCTCTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTC
TTCTGCACTCAAGTTTCCCAGTTTCCAATGACCCTCCCCGGTTGAGCCGGGGGCTTTCACATCAGACTTAAGAAACCGCC
TGCGAGCCCTTTACGCCCAATAATTCCGGACAACGCTTGCCACCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCG
TGGCTTTCTGGTTAGGTACCGTCAAGGTGCGAGCAGTTACTCTCGCACTTGTTCTTCCCTAACAACAGAGCTACGATC
CGAAAACCTTCATCACTCACGCGGCGTTGCTCCGTCAGACTTTCGTCCATTGCGGAAGANTCCCTACTGCTGCCTCCCGT
ANGAGTACTGGCCG ""


*Species Most Similar:
- Bacillus Zhangzhouensis Strain Tr91C
- Bacillus Zhangzhouensis Strian KBA10
- Bacillus Pumilus
- Bacillus Safensis
- Uncultured Bacillus


==Physiology and Pathogenesis==
==Lab Test Results==
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).<br>
'''Phenol Red Broth''': Glucose: positive, Sucrose: negative, Lactose: negative <br>
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br>
'''Starch Hydrolysis''': positive <br>
'''Casein Hydrolysis''': positive <br>
'''Gelatin Hydrolysis''': positive <br>
'''DNA Hydrolysis''': positive <br>
'''Lipid Hydrolysis''': positive <br>
'''Methyl Red''': negative <br>
'''Voges-Proskauer''': negative <br>
'''Citrate Test''': weak positive <br>
'''Sulfur Reduction''': negative <br>
'''Indole Production''': negative <br>
'''Motility Test''': positive <br>
'''Nitrate Reduction''': negative <br>
'''Urea Hydrolysis''': negative <br>
'''Triple Iron Sugar''': negative <br>
'''Mannitol Salt Agar''': positive <br>
'''Blood Agar''': positive <br>
 
==Interpretation of Results==
Based on results from tests performed in the microbiology lab, we were able to gather the following information about the soil organism: <br>
'''Biochemical characteristics:''' Phenol Red test results report that the soil organism ferments glucose. Citrate test results report that the soil organism has a slight ability to use citrate as its only carbon source. Motility test results report that the soil organism is motile. Mannitol Salt Agar results report that the soil ferments mannitol. Blood Agar results report that the soil organism breaks down red blood cells. <br>  
'''Enzymes made:''' Soil organism produces amylase, casease, gelatinase, DNAse, lipase and catalase enzymes. <br>


==References==
==References==
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]
1. [Branquinho R, Sousa C, Lopes J, Pintado ME, Peixe LV, Osório H. Differentiation of Bacillus pumilus and Bacillus safensis Using MALDI-TOF-MS. Desvaux M, ed. PLoS ONE. 2014;9(10):e110127. doi:10.1371/journal.pone.0110127.]
2. [http://en.wikipedia.org/w/index.php?title=Bacillus_safensis&oldid=660912220] Bacillus safensis. (2015, May 5). In Wikipedia, The Free Encyclopedia. Retrieved 20:41, May 8, 2015, from http://en.wikipedia.org/w/index.php?title=Bacillus_safensis&oldid=660912220]


==Author==
==Author==
Page authored by _____, student of Prof. Kristine Hollingsworth at Austin Community College.
Page authored by Kendahl Thomas and Sarah Perez, students of Prof. Kristine Hollingsworth at Austin Community College.


<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]

Latest revision as of 20:36, 8 December 2017

This student page has not been curated.

Classification

Domain : Bacteria
Division/phylum: Firmicutes
Class: Bacilli
Order: Bacillales
Family: Bacillaceae
Genus: Bacillus
Species: B. safensis

Species

NCBI: Taxonomy


Genus species

  • Terrabacteria Group

Molecule Type

  • Nucleic Acid

Habitat Information

  • Types of Soil Organism was Found In:
 Heiden Clay, 5-8% slopes, eroded (most common)
 Houston Black Clay, 1-3% slopes

Air Temp: 83 Degrees Fahrenheit/ 28 Degrees Celsius Humidity: 32 % 24 hr. Rainfall: 0.00in Pressure: 30.11 in/ 1018.5mb (sea level) Solar Radiation: 22.49 (mj/m^2) Wind at 4am: 2.06mph Wind at 4pm: 2.77mph

  • Soil Location Description:
Soil was taken from directly under a tree at Cabana Beach Apartments in San Marcos, Texas. This area is a very open area and gets water daily from sprinklers. The water it gets daily is minimal, so it does dry quickly. The soil was a very dark brown color. Had almost a dry dirt look and feel to it.

SoilMap.jpg

Description and Significance

  • Description:
Whiteish color, shiny, raised and semi flat

IMG 3267.jpg IMG 3417.jpg

Most common similar Bacteria: Bacillus Safensis: 

Bacillus Safensis is a Gram-positive, spore-forming rod bacterium. Bacillus safensis is also an aerobic, chemoheterotroph. Cell size ranges from 0.5-0.7 μm in diameter and 1.0-1.2 μm in length. Bacteria are motile, and use polar flagella for locomotion. Cells are considered mesophillic, as they can grow in temperatures ranging between 10-50 °C

It was first discovered in California and Florida on spacecraft and so is believed to have been brought to the USA from Mars. There are numerous strains of this bacterium, everyone belonging to the Firmicutes phylum of Bacteria. This organism is significant because it can tell us a bit about other planets and also how the bacteria spreads. It is also resistant to UV rays, gamma rays and is highly salt resistant, therefore it makes for a great plant growth-promoting rhizobacteria.

Genome Structure

DNA Sequencing Results: ""CCACCTGTCACTCTGTCCCCGAAGGGAAAGCCCTATCTCTAGGGTTGTCAGAGGATGTCAAGACCTGGT AAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAGT CTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCAGCACTAAGGGGCGGAAACCCCCTAACACTTAGCA CTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACAG ACCAGAGAGTCGCCTTCGCCACTGGTGTTCCTCCACATCTCTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTC TTCTGCACTCAAGTTTCCCAGTTTCCAATGACCCTCCCCGGTTGAGCCGGGGGCTTTCACATCAGACTTAAGAAACCGCC TGCGAGCCCTTTACGCCCAATAATTCCGGACAACGCTTGCCACCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCG TGGCTTTCTGGTTAGGTACCGTCAAGGTGCGAGCAGTTACTCTCGCACTTGTTCTTCCCTAACAACAGAGCTACGATC CGAAAACCTTCATCACTCACGCGGCGTTGCTCCGTCAGACTTTCGTCCATTGCGGAAGANTCCCTACTGCTGCCTCCCGT ANGAGTACTGGCCG ""

  • Species Most Similar:

- Bacillus Zhangzhouensis Strain Tr91C - Bacillus Zhangzhouensis Strian KBA10 - Bacillus Pumilus - Bacillus Safensis - Uncultured Bacillus

Lab Test Results

Phenol Red Broth: Glucose: positive, Sucrose: negative, Lactose: negative
Starch Hydrolysis: positive
Casein Hydrolysis: positive
Gelatin Hydrolysis: positive
DNA Hydrolysis: positive
Lipid Hydrolysis: positive
Methyl Red: negative
Voges-Proskauer: negative
Citrate Test: weak positive
Sulfur Reduction: negative
Indole Production: negative
Motility Test: positive
Nitrate Reduction: negative
Urea Hydrolysis: negative
Triple Iron Sugar: negative
Mannitol Salt Agar: positive
Blood Agar: positive

Interpretation of Results

Based on results from tests performed in the microbiology lab, we were able to gather the following information about the soil organism:
Biochemical characteristics: Phenol Red test results report that the soil organism ferments glucose. Citrate test results report that the soil organism has a slight ability to use citrate as its only carbon source. Motility test results report that the soil organism is motile. Mannitol Salt Agar results report that the soil ferments mannitol. Blood Agar results report that the soil organism breaks down red blood cells.
Enzymes made: Soil organism produces amylase, casease, gelatinase, DNAse, lipase and catalase enzymes.

References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

1. [Branquinho R, Sousa C, Lopes J, Pintado ME, Peixe LV, Osório H. Differentiation of Bacillus pumilus and Bacillus safensis Using MALDI-TOF-MS. Desvaux M, ed. PLoS ONE. 2014;9(10):e110127. doi:10.1371/journal.pone.0110127.]

2. [1] Bacillus safensis. (2015, May 5). In Wikipedia, The Free Encyclopedia. Retrieved 20:41, May 8, 2015, from http://en.wikipedia.org/w/index.php?title=Bacillus_safensis&oldid=660912220]

Author

Page authored by Kendahl Thomas and Sarah Perez, students of Prof. Kristine Hollingsworth at Austin Community College.

Retrieved from "https://microbewiki.kenyon.edu/index.php?title=Bacillus_Unknown&oldid=132325"