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{{Uncurated}}
<b>This Glogowski [[Microbial Biorealm]] <i>Genus </i>template page is a guide for each team when adding information and your reference(s) to an already existing species on MicrobeWiki. DO NOT EDIT OR WORK DIRECTLY ON THIS TEMPLATE PAGE.  COPY AND PASTE parts of this template that pertain to your information that you are submitting to the already existing species page and from there do your editing. Be sure to also copy and paste the bottom line provided on this template to add your team names on the line at the bottom of the species page that you edited after "Edited by" and in front of "students of M Glogowski at Loyola University". If the team is creating and adding an entirely new species to this website, COPY AND PASTE this entire template to set up the new page for that particular species, then begin to fill in and edit the page at that spot in MicrobeWiki and state at the bottom of the page that the new page is "Authored by [your Student Names]" in front of "students of M Glogowski at Loyola University".</b>
<b>This Glogowski [[Microbial Biorealm]] <i>Genus </i>template page is a guide for each team when adding information and your reference(s) to an already existing species on MicrobeWiki. DO NOT EDIT OR WORK DIRECTLY ON THIS TEMPLATE PAGE.  COPY AND PASTE parts of this template that pertain to your information that you are submitting to the already existing species page and from there do your editing. Be sure to also copy and paste the bottom line provided on this template to add your team names on the line at the bottom of the species page that you edited after "Edited by" and in front of "students of M Glogowski at Loyola University". If the team is creating and adding an entirely new species to this website, COPY AND PASTE this entire template to set up the new page for that particular species, then begin to fill in and edit the page at that spot in MicrobeWiki and state at the bottom of the page that the new page is "Authored by [your Student Names]" in front of "students of M Glogowski at Loyola University".</b>


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'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''
|}
|}
Kingdom: Bacteria
 
''Genus species''
Phylum: Proteobacteria
Class: Beta Proteobacteria
Order: Burkholderiales
Family: Ralstoniaceae
Genus: Ralstonia
Binomial name
Ralstonia paucula


==Description and significance==
==Description and significance==
Describe the appearance, habitat, etc. of the organism, and why you think it is important.
Describe the appearance, habitat, etc. of the organism, and why you think it is important.
Ralstonia paucula sp. R. paucula has been isolated from pool water, groundwater, and bottled mineral water and from clinical specimens. Despite its low pathogenicity, it is now recognized as an opportunistic pathogen which can generate serious infections such as septicemia, peritonitis, abscess, and tenosynovitis, particularly in immunocompromised patients


==16S Ribosomal RNA Gene Information==
==16S Ribosomal RNA Gene Information==
Describe the size and content of the 16S Ribosome.  How many genes?  Other interesting features?  What is known about its sequence for identification? Ralstonia paucula PCR-ribotyping and PCR-restriction fragment length polymorphism analysis of the spacer 16S-23S ribosomal DNA (rDNA) has been used
Describe the size and content of the 16S Ribosome.  How many genes?  Other interesting features?  What is known about its sequence for identification?
PCR-ribotyping was performed as described by Kostman et al. (7). The sequences of the 16S and 23S primers were 5'-TTGTACACACCGCCCGTCA-3' and 5'-GGTACCTTAGATGTTTCAGTTC-3' (7), respectively
 


==Cell structure and metabolism==
==Cell structure and metabolism==
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==Ecology==
==Ecology==
Habitat; symbiosis; contributions to the environment.
Habitat; symbiosis; contributions to the environment.
R. paucula has been isolated from pool water, groundwater, and bottled mineral water (2, 9, 17) and from clinical specimens


==Pathology==
==Pathology==
How does this organism cause disease?  Human, animal, plant hosts?  Virulence factors, as well as patient symptoms.
How does this organism cause disease?  Human, animal, plant hosts?  Virulence factors, as well as patient symptoms.
Ralstonia paucula (formerly CDC group IV c-2) has low pathogenicity, but it is now recognized as an opportunistic pathogen which can generate serious infections such as septicemia, peritonitis, abscess, and tenosynovitis, particularly in immunocompromised patients


==Current Research==
==Current Research==


Enter a summary of the most recent pertinent research here--at least one.
Enter a summary of the most recent pertinent research here--at least one.
Ralstonia paucula (Formerly CDC Group IV c-2): Unsuccessful Strain Differentiation with PCR-Based Methods, Study of the 16S-23S Spacer of the rRNA Operon, and Comparison with Other Ralstonia Species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum)
Recently, several authors have reported the use of PCR-ribotyping (3, 5, 7, 8, 12, 22, 23) and PCR-restriction fragment length polymorphism (PCR-RFLP) analysis (20, 21) for strain differentiation within various bacterial species. the stydy we evaluated the ability to distinguish between R. paucula strains and between strains belonging to other Ralstonia species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum). As the ribosomal DNA (rDNA) intergenic spacer can contain genes coding for tRNA (15, 24), we also explored this region in R. paucula and compared it to those of other Ralstonia species.


==References==
==References==
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<!-- Do not edit or remove this line-->[[Category:Pages edited by students of Mary Glogowski at Loyola University]]
<!-- Do not edit or remove this line-->[[Category:Pages edited by students of Mary Glogowski at Loyola University]]
1- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC87738/

Latest revision as of 12:58, 17 October 2017

This student page has not been curated.

This Glogowski Microbial Biorealm Genus template page is a guide for each team when adding information and your reference(s) to an already existing species on MicrobeWiki. DO NOT EDIT OR WORK DIRECTLY ON THIS TEMPLATE PAGE. COPY AND PASTE parts of this template that pertain to your information that you are submitting to the already existing species page and from there do your editing. Be sure to also copy and paste the bottom line provided on this template to add your team names on the line at the bottom of the species page that you edited after "Edited by" and in front of "students of M Glogowski at Loyola University". If the team is creating and adding an entirely new species to this website, COPY AND PASTE this entire template to set up the new page for that particular species, then begin to fill in and edit the page at that spot in MicrobeWiki and state at the bottom of the page that the new page is "Authored by [your Student Names]" in front of "students of M Glogowski at Loyola University".

Classification

Higher order taxa

Domain; Phylum; Class; Order; family [Others may be used. Use NCBI link to find]

Species

NCBI: Taxonomy

Genus species

Description and significance

Describe the appearance, habitat, etc. of the organism, and why you think it is important.

16S Ribosomal RNA Gene Information

Describe the size and content of the 16S Ribosome. How many genes? Other interesting features? What is known about its sequence for identification?


Cell structure and metabolism

Interesting features of cell structure; how it gains energy; what important molecules it produces.


Ecology

Habitat; symbiosis; contributions to the environment.

Pathology

How does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.

Current Research

Enter a summary of the most recent pertinent research here--at least one.

References

It is required that you add at least one journal reference (in same format as the sample reference below) that corresponds to the info that you added to this page.

[Sample reference] Faller, A., and Schleifer, K. "Modified Oxidase and Benzidine Tests for Separation of Staphylococci from Micrococci". Journal of Clinical Microbiology. 1981. Volume 13. p. 1031-1035.

Edited by students of M Glogowskiat Loyola University

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