Endozoicomonas ascidiicola

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Classification

Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Oceanospirillales; Endozoicomonadaceae [1]

Species

NCBI: [2]

Genus species

Description and Significance

E. ascidiicola is represented by the type strain AVMART05T and strain KASP37. It is a Gram-negative (KOH positive), motile, rod-shaped bacterium, measuring 0.3–0.7um in diameter and 1.2–11.3um long. Colonies can be beige, circular and convex with a smooth surface and entire margins (strain AVMART05T) or transparent-white, circular and flat with a smooth surface and undulate margins (strain KASP37). Colonies measure 0.5–1 mm (AVMART05T) or 2–3 mm (KASP37) in diameter on marine agar after a 72 h incubation at 25◦C.

E. ascidiicola is mesophilic, neutrophilic, and slightly halophilic, with optimal growth occurring at 23–25◦C, pH 6–7, and 1–2% NaCl. Cells are facultative anaerobic and reduce nitrate.

Genome Structure

E. ascidiicola has a singular, circular genome. The genome contains at least three distinct 16S rRNA gene paralogs. The major cellular fatty acids are C18:1 7c, C16:1 7c, and C16:0. The only detected respiratory quinone is Q-9. The polar lipids comprise phosphatidylglycerol (PG), phosphatidylethanolamin (PE), and one phosphoaminolipid. Most prominent sites of sequence divergence are located in the V2 and V3 region of the 16S rRNA genes. The DNA G+C content is ~47 mol%.

Cell Structure, Metabolism and Life Cycle

The peptidoglycan type is A1. Grows well on full and half-strength marine agar and marine broth. Grows well on porcine mucin agar

Ecology and Pathogenesis

Ascidiicola = Ascidian-dweller Facultative symbiotint within invertebrates, including ascidians. They have also been detected free-living in the seawater, suggesting ascidians can hortizonally acquire these microbes. This particular species is ascidian host-specific. It was first isolated from the pharynx tissue of the ascidian genus Ascidiella collected from Gullmarsfjord, Sweden in 2010. E. ascidiicola is a chemoorganohetherotroph and can grow on pharynx-produced mucin. biogeochemical significance; contributions to environment.

References

Schreiber L, Kjeldsen KU, Obst M, Funch P, Schramm A. 2016. Description of Endozoicomonas ascidiicola sp. nov., isolated from Scandinavian ascidians. Systematic and Applied Microbiology 39:313–318.

Schreiber L, Kjeldsen KU, Funch P, Jensen J, Obst M, López-Legentil S, Schramm A. 2016. Endozoicomonas Are Specific, Facultative Symbionts of Sea Squirts. Front Microbiol 7.

Author

Page authored by Grace Kennedy, student of Prof. Bradley Tolar at UNC Wilmington.