Rhizobium etli: Difference between revisions
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Rhizobium Etli is one of the many soil-living bacteria able to live in conditions of nitrogen limitation due to its distinctive ability to settle onto root nodules of legumes. | Rhizobium Etli is one of the many soil-living bacteria able to live in conditions of nitrogen limitation due to its distinctive ability to settle onto root nodules of legumes. | ||
Like other rhizobia, it is characterized as aerobic, gram negative and able to form symbiotic relationship with legumes. In specific, rhizobium etli is the predominant bacteria found legumes such as the common bean, P. Vulgaris. | Like other rhizobia, it is characterized as aerobic,gram negative and able to form symbiotic relationship with legumes. (1, 2) In specific, rhizobium etli is the predominant bacteria found legumes such as the common bean, P. Vulgaris. (3) | ||
Rhizobium Etli is found world wide and discovered as early as 16th century. Due to its prehistoric existence, attempts to identify origin of the species was performed by identifying its molecular marker. This was performed by searching a diversity within different rhizobium etli species from P. Vulgaris. Isolation of the rhizobia strain from the nodule of the root of the plant was removed, sterilized with ethanol and hydrogen peroxides, and grown on YEM-Congo red agar medium. Isolation and identification was done by 16S rRNA-encoding DNA-RFLP analysis. The analysis showed most to be from species rhizobium etli. The nodC gene was identified and isolated to be used as a molecular marker. Results from the experiment showed rhizobium etli is not only found in the America’s but also identified in parts of Africa, Asia, and Europe. | Rhizobium Etli is found world wide and discovered as early as 16th century. Due to its prehistoric existence, attempts to identify origin of the species was performed by identifying its molecular marker. This was performed by searching a diversity within different rhizobium etli species from P. Vulgaris. Isolation of the rhizobia strain from the nodule of the root of the plant was removed, sterilized with ethanol and hydrogen peroxides, and grown on YEM-Congo red agar medium. Isolation and identification was done by 16S rRNA-encoding DNA-RFLP analysis. The analysis showed most to be from species rhizobium etli. The nodC gene was identified and isolated to be used as a molecular marker. Results from the experiment showed rhizobium etli is not only found in the America’s but also identified in parts of Africa, Asia, and Europe. (3) | ||
Rhizobium Etli is important enough to have its genome sequence because of its unique ability to form symbiotic relationship with legumes. The detail in which it performs this will be in the cell structure and metabolism section. To give a general idea of its importance, the host benefits by being provided nitrogen in the form of ammonia from the bacteria, while the bacteria is provided carbon and nutrients from the host. | Rhizobium Etli is important enough to have its genome sequence because of its unique ability to form symbiotic relationship with legumes. The detail in which it performs this will be in the cell structure and metabolism section. To give a general idea of its importance, the host benefits by being provided nitrogen in the form of ammonia from the bacteria, while the bacteria is provided carbon and nutrients from the host. (2) | ||
In agriculture, crop rotation and soil fumigation is performed each year to prevent diseases. Futher knowledge of the bacteria would allow possible genetic engineering onto the bacteria to possibly work as an antibiotic. | In agriculture, crop rotation and soil fumigation is performed each year to prevent diseases. Futher knowledge of the bacteria would allow possible genetic engineering onto the bacteria to possibly work as an antibiotic. (4) | ||
==Genome structure== | ==Genome structure== | ||
Revision as of 23:51, 26 August 2007
A Microbial Biorealm page on the genus Rhizobium etli
Classification
Higher order taxa
Bacteria (Domain); Proteobacteria (Phylum); Alphaproteobacteria (Class); Rhizobiales (Order); Rhizobiaceae (family)
Species
Rhizobium Etli
Description and significance
Rhizobium Etli is one of the many soil-living bacteria able to live in conditions of nitrogen limitation due to its distinctive ability to settle onto root nodules of legumes. Like other rhizobia, it is characterized as aerobic,gram negative and able to form symbiotic relationship with legumes. (1, 2) In specific, rhizobium etli is the predominant bacteria found legumes such as the common bean, P. Vulgaris. (3)
Rhizobium Etli is found world wide and discovered as early as 16th century. Due to its prehistoric existence, attempts to identify origin of the species was performed by identifying its molecular marker. This was performed by searching a diversity within different rhizobium etli species from P. Vulgaris. Isolation of the rhizobia strain from the nodule of the root of the plant was removed, sterilized with ethanol and hydrogen peroxides, and grown on YEM-Congo red agar medium. Isolation and identification was done by 16S rRNA-encoding DNA-RFLP analysis. The analysis showed most to be from species rhizobium etli. The nodC gene was identified and isolated to be used as a molecular marker. Results from the experiment showed rhizobium etli is not only found in the America’s but also identified in parts of Africa, Asia, and Europe. (3)
Rhizobium Etli is important enough to have its genome sequence because of its unique ability to form symbiotic relationship with legumes. The detail in which it performs this will be in the cell structure and metabolism section. To give a general idea of its importance, the host benefits by being provided nitrogen in the form of ammonia from the bacteria, while the bacteria is provided carbon and nutrients from the host. (2)
In agriculture, crop rotation and soil fumigation is performed each year to prevent diseases. Futher knowledge of the bacteria would allow possible genetic engineering onto the bacteria to possibly work as an antibiotic. (4)
Genome structure
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Does it have any plasmids? Are they important to the organism's lifestyle?
Cell structure and metabolism
Describe any interesting features and/or cell structures; how it gains energy; what important molecules it produces.
Ecology
Describe any interactions with other organisms (included eukaryotes), contributions to the environment, effect on environment, etc.
Pathology
How does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.
Application to Biotechnology
Does this organism produce any useful compounds or enzymes? What are they and how are they used?
Current Research
Enter summaries of the most recent research here--at least three required
References
Edited by student of Rachel Larsen
