Staphylococcus saprophyticus: Difference between revisions
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As of 2005, the genome of ''S. saprophyticus'' (strain ATCC 15305) has been fully sequenced by Japanese researchers. [1] ''S. saprophyticus'' contains a circular chromosome of 2,516,575 bp, 2,446 ORF’s, and two plasmids 38.4 and 22.9 kb in size | As of 2005, the genome of ''S. saprophyticus'' (strain ATCC 15305) has been fully sequenced by Japanese researchers. [1] ''S. saprophyticus'' contains a circular chromosome of 2,516,575 bp, 2,446 ORF’s, and two plasmids 38.4 and 22.9 kb in size. The genome was sequenced to better understand the parthenogenesis of the organism. Whole-genome shotgun sequencing was used, sequencing in 1-2 kb or 10 kb inserts. The inserts were then assembled using PHRED/PHRAP/CONSED programs. The gaps were filled by PCR direct sequencing, using specific primers at the ends of each gap. [1] | ||
The genome of ''S. saprophyticus'' contains many mobile elements including Staphylococcal cassette chromosomes (SCCs), an insertion sequence and a genomic island. The SCCs are thought to have integrated into the genome through a two-step process and contain a restriction enzyme modification system and a cassette chromosome recombinase (Ccr). [1] The SCCs are responsible for contributing to pathenogenicity and antibiotic resistance in Staphylococci. The genomic island in S. saprophyticus is associated with resistance to the antibiotics streptomycin and fosfomycin unlike the genomic islands in other Staphylococci genomes, which are associated with pathenogenicity, such as in Staphylococci aureus. [2] These mobile elements allow for lateral gene transfer between other bacterial species. | The genome of ''S. saprophyticus'' contains many mobile elements including Staphylococcal cassette chromosomes (SCCs), an insertion sequence and a genomic island. The SCCs are thought to have integrated into the genome through a two-step process and contain a restriction enzyme modification system and a cassette chromosome recombinase (Ccr). [1] The SCCs are responsible for contributing to pathenogenicity and antibiotic resistance in Staphylococci. The genomic island in S. saprophyticus is associated with resistance to the antibiotics streptomycin and fosfomycin unlike the genomic islands in other Staphylococci genomes, which are associated with pathenogenicity, such as in Staphylococci aureus. [2] These mobile elements allow for lateral gene transfer between other bacterial species. | ||
Revision as of 03:45, 29 August 2007
A Microbial Biorealm page on the genus Staphylococcus saprophyticus
Classification
Higher order taxa
Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae
Species
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NCBI: Taxonomy |
Staphylococcus saprophyticus
Description and significance
Describe the appearance, habitat, etc. of the organism, and why it is important enough to have its genome sequenced. Describe how and where it was isolated. Include a picture or two (with sources) if you can find them.
Genome structure
![Staphylcoccus saprophyticus genome. From [1]](/index.php/File:S.saprophyticus_genome.png)
As of 2005, the genome of S. saprophyticus (strain ATCC 15305) has been fully sequenced by Japanese researchers. [1] S. saprophyticus contains a circular chromosome of 2,516,575 bp, 2,446 ORF’s, and two plasmids 38.4 and 22.9 kb in size. The genome was sequenced to better understand the parthenogenesis of the organism. Whole-genome shotgun sequencing was used, sequencing in 1-2 kb or 10 kb inserts. The inserts were then assembled using PHRED/PHRAP/CONSED programs. The gaps were filled by PCR direct sequencing, using specific primers at the ends of each gap. [1]
The genome of S. saprophyticus contains many mobile elements including Staphylococcal cassette chromosomes (SCCs), an insertion sequence and a genomic island. The SCCs are thought to have integrated into the genome through a two-step process and contain a restriction enzyme modification system and a cassette chromosome recombinase (Ccr). [1] The SCCs are responsible for contributing to pathenogenicity and antibiotic resistance in Staphylococci. The genomic island in S. saprophyticus is associated with resistance to the antibiotics streptomycin and fosfomycin unlike the genomic islands in other Staphylococci genomes, which are associated with pathenogenicity, such as in Staphylococci aureus. [2] These mobile elements allow for lateral gene transfer between other bacterial species.
Cell structure and metabolism
Describe any interesting features and/or cell structures; how it gains energy; what important molecules it produces.
Ecology
Describe any interactions with other organisms (included eukaryotes), contributions to the environment, effect on environment, etc.
Pathology
How does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.
Application to Biotechnology
Does this organism produce any useful compounds or enzymes? What are they and how are they used?
Current Research
Enter summaries of the most recent research here--at least three required
References
[2] Novick, R. P. (2003) Plasmid 49:, 93-105.
Edited by Paul Wong, student of Rachel Larsen, UCSD.
