Methanosphaera stadtmanae: Difference between revisions
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The Methanosphaera stadtmanae strain DSZM 3091 was isolated by the Deutsche Sammlung von Zellkulturen und Mikroorganismen (DSMZ), located in Braunschweig, Germany. It is important to isolate and sequence the genome of this archaeon as it is the first human archaeal commensal; therefore, it can help researchers gain a better understanding of the role of archael commensals in humans. It was found that M. stadtmanae inhabits the human intestine. These archaea thrive there because methanol is present as a by product of “pectin degradation by Bacteroides species and other anaerobic bacteria” | |||
It was found that the M. stadtmanae can be grown on a medium that contains 0.5 g/liter sodium formate and 10% rumen fluid. The process of sequencing M. stadtmanae included extracting and shearing “its total genomic DNA to obtain various shotgun data using 3 kb to 5 kb fractions.” Next, the fragments were cloned into vectors produced by the Invitrogen Co., pCR4-TOPO. Then, the ends of the recombinant plasmids were sequenced using dye terminator chemistry. Also, in order to edit the sequence, part of the Staden software package, GAP4, was used. In fact, about 8.7-fold coverage of the genome was achieved after reconstructing 21,555 sequences. | |||
==Genome structure== | ==Genome structure== | ||
Revision as of 20:28, 4 June 2007
A Microbial Biorealm page on the genus Methanosphaera stadtmanae
Classification
Higher order taxa
Kingdom: Biota Domain: Archaea Phylum: Euryarchaeota Class: Methanobacteria Order: Methanobacteriales Family: Methanobacteriaceae
Genus
Genus: Methanosphaera Species: stadtmanae
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NCBI: Taxonomy |
The Methanosphaera stadtmanae strain DSZM 3091 was isolated by the Deutsche Sammlung von Zellkulturen und Mikroorganismen (DSMZ), located in Braunschweig, Germany. It is important to isolate and sequence the genome of this archaeon as it is the first human archaeal commensal; therefore, it can help researchers gain a better understanding of the role of archael commensals in humans. It was found that M. stadtmanae inhabits the human intestine. These archaea thrive there because methanol is present as a by product of “pectin degradation by Bacteroides species and other anaerobic bacteria” It was found that the M. stadtmanae can be grown on a medium that contains 0.5 g/liter sodium formate and 10% rumen fluid. The process of sequencing M. stadtmanae included extracting and shearing “its total genomic DNA to obtain various shotgun data using 3 kb to 5 kb fractions.” Next, the fragments were cloned into vectors produced by the Invitrogen Co., pCR4-TOPO. Then, the ends of the recombinant plasmids were sequenced using dye terminator chemistry. Also, in order to edit the sequence, part of the Staden software package, GAP4, was used. In fact, about 8.7-fold coverage of the genome was achieved after reconstructing 21,555 sequences.
Genome structure
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Does it have any plasmids? Are they important to the organism's lifestyle?
Bacillus Licheniformis is a Gram positive, thermophillic bacterium. Its optimal growth temperature is 50°C, but it can also survive at much higher temperatures. Its optimal temperature for
Cell structure and metabolism
Describe any interesting features and/or cell structures; how it gains energy; what important molecules it produces.
Ecology
Describe any interactions with other organisms (included eukaryotes), contributions to the environment, effect on environment, etc.
Pathology
How does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.
Application to Biotechnology
Does this organism produce any useful compounds or enzymes? What are they and how are they used?
Current Research
Enter summaries of the most recent research here--at least three required
References
Edited by student of Rachel Larsen and Kit Pogliano
