Lactobacillus plantarum and its biological implications

Introduction

Figure 1. Electron micrograph of L. plantarum[1]

By Katie Adlam

Lactobacillus plantarum (L. plantarum) is a rod-shaped, gram-positive lactic acid bacterium from the spirochete phylum. It is commonly found in the human and other mammalian gastrointestinal tracts, saliva, and various food products. It can grow at temperatures between 15-45˚C and at pH levels as low as 3.2 [1]. L. plantarum is a facultative heterofermentative [2,3] that ferments sugars to produce lactic acid, ethanol or acetic acid, and carbon dioxide under certain conditions and selective substrates. Depending on the carbon source, these bacteria can switch from using heterofermentative and homofermentative ways of metabolism. This bacterium is acid and bile salt tolerant, which allows it to survive the passage through the gastrointestinal tract of humans. L. plantarum is a current interest to researchers and the food industry since it is considered a safe probiotic. It can help limit the amount of pathogenic bacteria or diseases that can have a negative impact on humans. In addition, recent research is indicating that L. plantarum can be used as a vaccine vehicle.

Genome

Figure 2. Genome-atlas view of the L. plantarum WCFS1 chromosome. The outer to inner circles show (i) positive strand ORFs (red); (ii) negative strand ORFs (blue); (iii) GC-skew (green); (iv) G+C content (black); (v) prophage-related functions (green) and IS-like elements (purple); and (vi) rDNA operons (black) and tRNA encoding genes (red). Kleerebezem 2003.[2]

L. plantarum has a relatively large genome compared to other Lactobacillus spp. Its genome consists of a 3.3 Mb chromosome, which is the largest sequenced genome of any lactic acid bacteria [2]. The genome of L. plantarum consists of five rRNA operons, which are evenly distributed around the chromosome (FIGURE 2) [1]. 62 tRNA encoding genes have been found and are in relation to some of the rRNA clusters. In addition, the genome encodes two classes of transposase regions, which are thought to encode mobile genetic elements [1].

The genome consists of 3,052 protein-encoding genes and only 39 of these genes are pseudogenes [1]. L. plantarum proteins are very similar to other Gram-positive bacteria since they have low GC content, have a peptidoglycan cell wall, and they are organized collinear. L. plantarum genome contains genes for the Embden-Meyerhoff-Parnas (EMP) pathway and some of the genes correlate to enzymes that break down pentoses and hexoses. Furthermore, its genome shows that it has genes that encode for phosphotransferase, mannose, and fructose transport systems [1].

Metabolism

Figure 3. Lactate and acetate production pathways in L. plantarum. EMP, Embden–Meyerhof Parnas pathway; LDH, lactate dehydrogenase; POX, pyruvate oxidase; PDH, pyruvate dehydrogenase; PFL, pyruvate formate lyase; PTA, phosphotransacetylase; AK, acetate kinase. Quatravaux 2006.[3]

L. plantarum is a facultative anaerobe or a facultative heterofermentative, as it can grow in the presence and absence of oxygen. It has enzymes for fermentation through the Embden-Meyerhoff-Parnas pathway (Figure 3) or through the phosphoketolase pathway, which allows this bacterium to perform homolactic and heterolactic fermentation [1]. When oxygen is not present and this bacterium undergoes homolactic-like fermentation, L. plantarum converts carbon sources to D and L- forms of lactate and alcohols via lactate dehydrogenase. This normally occurs during its growth phase. It is important to note that L. plantarum can use a variety of carbon sources, which is hypothesized to be the result of horizontal gene transfer.

Under aerobic conditions, lactate is converted to acetate and one ATP is produced via lactate dehydrogenase, pyruvate oxidase, and acetate kinase enzymes. In addition to producing acetate, this pathway also forms hydrogen peroxide and carbon dioxide as byproducts. Hydrogen peroxide is formed by the conversion of oxygen via a manganese-dependent process and the production of this radical species can kill off other bacteria. Manganese-dependent processes use manganese as a catalase to lower the concentration of oxygen, which is beneficial to the aerotolerant bacterium [4]. L. plantarum can produce a variety of products from fermentation due to a large “pyruvate-dissipating potential” [1]. In addition, research has found that L. plantarum has a fumarate reductase, which implies that this bacterium has a basic electron transport chain for anaerobic bacteria [1]. It is proposed that this bacterium possesses a nitrate reduction system, which could use nitrate as an electron acceptor. Furthermore, it does not possess the citric acid cycle, which is a cycle that aerobic organisms use to generate energy through oxidation of acetate.

Biosynthesis and Secretion

Figure 4. Schematic representation of the human gastrointestinal tract (GI tract). Daniel 2011.[4]

L. plantarum is typically found in a protein-rich environment, such as in yogurt, so it has uptake systems for peptides. Once these peptides are taken up, various types of peptidases degrade them inside the bacterium. L. plantarum has 19 different genes encoding peptidases that have different specific functions, three of which can cleave N-terminal proline residues. Even though this bacterium has protein degradation mechanisms, it is still capable of producing most amino acids, except branched-chain amino acids such as valine, leucine, and isoleucine [1].

In addition to synthesizing amino acids, L. plantarum can also perform a nonribosomal peptide synthesis, which is independent of mRNA [1]. Before this discovery, no other lactic acid bacteria was known to use this sort of biosynthesis machinery. The L. plantarum nonribosomal peptide synthesis cluster contains two nonribosomal synthesis proteins, an important phosphopantetheinyl transferase, and proteins that are needed for precursors for regulation, transport, and enzymes [1].

Amino acids and peptides in L. plantarum are transported mainly through 57 ATP-binding cassette (ABC) transporters, which 27 are importers and 30 are exporters. Since this bacterium cannot synthesize its own branched chain amino acids, it is able to uptake them through these transporters. In addition, L. plantarum has a high number of sugar import systems and regulatory proteins, which could be responsible for how it is a flexible and versatile bacterium, as it can grow by using different carbon sources and can adapt to a wide array of environments [1].

L. plantarum is known to be able to adapt to stressful environments such as those in the gastrointestinal tract with a low pH or high salt content (Figure 4). Its genome encodes for proteases that can degrade abnormal or nonfunctional proteins, as well as heat and cold-shock proteins to save energy under stress and to survive different climates. In order to survive in acidic environments, this bacterium uses the F_oF₁-ATPase and sodium-proton pumps to help regulate and maintain the intracellular pH. Furthermore, it has alkaline shock proteins to assist in pH tolerance. In addition, L. plantarum has developed ways to deal with oxidative stress by having catalases, peroxidases, and reductases, as well as a high intracellular concentration of manganese ions (Mn²⁺) to scavenge oxygen radicals [1]. This bacterium is able to obtain and accumulate manganese ions due to the P-type manganese translocating ATPase.

In addition to being able to adapt to the environment, L. plantarum has surface proteins that allow it to interact with the environment. It has a Sec transport system that is comprised of SecA/SecE/SecG/SecY/YajC (no SecDF) and it also has other proteins involved in secretion such as peptidases [1]. In addition, there are genes that encode for a sortase, which is an enzyme that recognizes and cleaves a carboxy-terminal sequences to modify the surface proteins to interact with different surfaces and substrates for growth. Lastly, L. plantarum has a protein machinery that is used to bind and uptake DNA from the environment. Scientists hypothesize that it acquired its ability to adapt to many different environments from this uptake machinery. Researchers also have found very large regions in genome of L. plantarum where there is unusual base composition when comparing it to closely related species. An example of this are the genes for sugar uptake and catabolism (1). This reflects how L. plantarum is a very versatile species that can adjust to its environment.

Food Production

Figure 5. A) Effect of strains 16 and 62 on the growth of R. mucilaginosa in orange juice. B) Effect of strains 16 and 62 on the growth of R. mucilaginosa in yoghurt. Control juice (squares), strain 16 (circles), strain 62 (triangles).Crowley 2012.[5]

L. plantarum is commonly used in dairy, meat, and plant fermentations, and is found in food products such as yogurt, cheese, kimchi, sauerkraut, sourdough, and pickles [3, 5, 6]. The bacterium gives food certain tastes and flavors depending on the balance between acetate (volatile) and lactate (nonvolatile) organic acids [2]. By using oxygen and aerating food, producers can control the amount of acetic acid that is produced as the final product of fermentation. An example of this is sourdough fermentation. The bacterium is mainly used for dough acidification, but it can play a role in the leavening process.

A specific example of a food item that is produced by L. plantarum fermentation is sauerkraut. Sauerkraut is spontaneously produced from cabbage through lactic acid bacteria that are acid tolerant homolactic fermenters [7]. However, the taste of sauerkraut varies depending on the type of bacteria used, substrate for fermentation, salt concentration, and temperature of the fermentation. Because of the variation, a study set out to find starter cultures that could be universally used to produce sauerkraut. Leuconostoc mesenteroides (L. mesenteroides) was used for the first phase of fermentation and L. plantarum for the second. L. mesenteroides provides a mild, pleasant aromatic flavor to the sauerkraut while L. plantarum gives it an acidic, vinegar taste. The researchers found that by using both bacteria that the fermentation time was shortened compared to other fermentation processes [7]. In addition,L. plantarum being dominant in the acidic, second phase of the fermentation resulted in the inhibition on the growth of other microorganisms. These two starter bacteria improved the final product of sauerkraut by reducing the fermentation time and the growth of pathogenic microbes.

It is also a current interest of consumers that more naturally preserved products are available. Because of this, scientists are studying antifungal lactic acid bacteria and their preservative properties. Since lactic acid bacteria typically can survive at wide range of temperatures and pH levels where some food spoilage yeasts are found, lactic acid bacteria are being studied to determine if they can inhibit growth of undesirable yeast. L. plantarum, a lactic acid bacterium, produces antifungal activity that can be substituted for potentially harmful preservatives that are found in food products [8]. A study in 2012 examined L. plantarum to determine if it had the ability to inhibit the growth of Rhodotorula mucilaginosa (R. mucilaginosa), a yeast that spoils dairy products and orange juice, in different refrigerated foods. After isolating and determining the viability of two strands of L. plantarum (strand 16 and 62), this group determined its affect on the activity of R. mucilaginosa by using the antiyeast bacteria as dairy starter adjuncts in yogurt and inoculants in orange juice. Inhibition of R. mucilaginosa by L. plantarum was compared against commercial, FDA approved preservatives such as sodium benzoate and potassium sorbate. The study demonstrated that L. plantarum, in fact, had a more significant impact on the inhibition of the growth of R. mucilaginosa compared to the FDA preservatives and it decreased the rate of spoilage in both orange juice and yogurt (Figure 5) [8]. Since the inhibition of R. mucilaginosa was more effective than sodium benzoate and potassium sorbate, this bacterium has the potential to increase shelf life of yogurt and orange juice. However, it is important to account that the exact antifungal compounds that L. plantarum produces is unknown and is currently being investigated.

Probiotics and Biotherapeutic Applications

According to the Merriam-Webster Dictionary, a probiotic is a dietary supplement that contains live bacteria or yeast and is used to maintain the normal gastrointestinal flora [9]. It is often taken orally and helps restore the flora after infection or after antibiotic use. Some individuals, such as pregnant women, infants, and children with neurodevelopmental disorders, can benefit greatly from the use of probiotics [5].

In order for probiotics to be effective, they must be able to reach their target site after ingestion (Figure 3). They have to travel through the gastrointestinal tract and the stomach, where the low pH could disrupt the energy supply of some bacteria since the proton motive force or enzyme function could be affected. In addition, probiotics must go through the small intestine that contains bile salts that can act as surfactants and can disturb cell membranes, DNA, and RNA [10]. They also must be somewhat resistant to antibiotics so that they can maintain the gastrointestinal tract homeostasis and aid in the recovery of intestinal microflora with treatment of antibiotics [11]. However, it is important to recognize that the antibiotic resistance genes could get transferred and form highly antibiotic resistant pathogenic bacteria.

L. plantarum is considered a probiotic because it secretes antimicrobial compounds, such as bacteriocin, that inhibit pathogenic gram-positive and gram-negative colonies from forming [5]. Bacteriocin, which is a Lactobacillus-inhibitory factor and toxin, inhibits the growth of similar bacteria and other antibiotic-like substances. L. plantarum also has a mannose-specific adhesion, which allows it to adhere to the epithelial lining in the human intestines and compete with both gram-positive and gram-negative pathogenic bacteria for nutrients in the epithelial lining [5,12]. These traits, in addition to L. plantarum being pH and temperature tolerant, make it a potential probiotic to be used for therapy for those who suffer from gastrointestinal diseases such as Crohn’s Disease, inflammatory bowel disease (IBD), and colitis [5, 6, 11, 13, 14].

Gastrointestinal Tract

Probiotics have been shown to help in lactose digestion and in diseases such as Crohn’s Disease, inflammatory bowel disease, and colitis [5, 6, 11, 13, 14]. These conditions usually occur due to the epithelial lining of the intestines becoming stressed or when homeostasis is not obtained. This leads to damage of the lining and increases permeability of the lining. Furthermore, this allows pathogenic bacteria to stimulate a mucosal immune response. The endotoxin and DNA from the pathogenic bacteria can cause an inflammatory response of the epithelial lining by bacterial translocation [14] and by the endotoxins stimulating the release of cytokines and other pro-inflammatory mediators. For example, when tumor neurosis factor-α (TNF-α), a cytokine, becomes stimulated, it causes a potent interleukin (IL-8) to be released [15]. The secretion of the chemokine causes the movement of inflammatory cells into the lining. Furthermore, this response is mediated by an extracellular signal-regulated kinase (ERK). However, this inflammatory response can be repressed by species such as Lactobacilli, and specifically, L. plantarum [14].

Figure 6. Hematoxylin-eosin stained gastric sections (×20). A) Control group. B) H. pylori infected group. C and D) L. plantarum treated groups showed improvements in gastric inflammation. Infiltration of inflammatory cells (arrows) GE: Gastric epithelium; LP: Lamina propria; MM: Muscularis mucosae; SM: Submucosa; ML: Muscularis.Sunanliganon 2012.[6]

Current research is determining the probiotic effects of L. plantarum on the epithelial lining of the intestines and how it can benefit humans [6, 11, 13, 14, 16]. In 2007, a study established that L. plantarum could suppress an inflammatory response of the intestinal epithelial cells by inhibiting ERK activation of TNF-α, which would reduce the signaling of chemokines (IL-8) [15]. These results indicate that L. plantarum, as a probiotic, could help maintain the epithelial barrier and inhibit the inflammatory response (Figure 5). Furthermore, it was determined that L. plantarum could be used to prevent the development of colitis since it has a protective effect on tight junction (TJ) associated proteins and TJ ultra-structure [14]. It has been hypothesizes that the TJs might form a barrier in the epithelial layer that could prevent bile from penetrating the blood stream [16]. In other words, L. plantarum is capable of providing protection on the intestinal epithelium by reducing the permeability of the intestinal lining and can reduce the amount of pro-inflammatory proteins in the gastrointestinal tract.

A specific bacterium that damages the mucosa and causes an inflammatory response by increasing TNF-α and interleukin is Helicobacter pylori (H. pylori). The bacterium colonizes in the human gastric mucosa and causes gastritis and ulcer diseases [6]. Its lipopolysaccharides (LPS) and surface proteins stimulate the production of TNF-α, interleukin, and reactive oxygen species (ROS). These do not stay localized to the area of infection, but they can spread throughout the body and cause damage. A study in 2012 showed that L. plantarum has anti-Helicobacter activity and can inhibit the growth of the pathogenic bacterium in acidic environments (pH 4) (Figure 6). Some fermentation products, such as lactic acid, inhibit the urease activity capability of H. pylori, as well as Lactobacillus-inhibitory factors such as bacteriocin [6]. By having anti-Helicobacter activity, L. plantarum can be used as a therapy for gastritis and other gastrointestinal tract diseases.

Vaccines

Recombinant strains of lactic acid bacteria are used to produce therapeutic proteins and to deliver these proteins to mucosal sites, where they can safely persist [17, 18]. These mucosal surfaces are a major site of pathogenic entry and both systemic and mucosal immune responses can be induced at these surfaces [18]. They are also used to secrete other proteins such as interleukins and antibodies. In addition, mucosal vaccines can cause IgA secretion and a systemic immune response with T-cells to stimulate the immune system [19].

For example, bacteria expressing allergens provide an advantageous method of immunotherapy compared to subcutaneous and sublingual therapies [17, 20]. It is beneficial because the allergen will be protected from proteases since it is contained within the bacteria, it is cost-effective since the antigen does not have to be processed, and the components of the allergen and the lactic acid bacteria are presented at the same time at the immune response. Studies have shown that certain strains of L. plantarum can be used as delivery vehicles with an allergen to prevent certain allergies, such as dust mite allergies [20]. If a recombinant strain has the dust mite allergen (Der p 1) for antigen delivery, it can reduce or prevent the stimulation of the ERK-pathway, which would lead to suppression of the chemokines that would cause inflammation.

Figure 7. Antibody response to oral administration of recombinant L. plantarum: mucosal IgA. Rio 2010.[7]

In addition, proinflammatory recombinant strains of L. plantarum are hypothesized to be effective mucosal delivery vehicles for vaccine antigens [18]. For example, a recent study used a strain of L. plantarum that expressed invasin, from Yersinia pseudotuberculosis (Y. pseudotuberculosis). Invasin is a virulence factor that binds to β1-integrins on the surface of microfold cells (M cells) and stimulates the uptake of Y. pseudotuberculosis in the intestines. It can also cause inflammation of the host cell by activating the innate immune system [18]. By modifying L. plantarum so that the extracellular domain of invasin is anchored to the bacterial surface by N-terminal anchoring motifs, this strain of the bacterium can imitate the early infection symptoms of Y. pseudotuberculosis and this can increase adjuvant characteristics. In other words, this strain of L. plantarum can, through the use of different N-terminal anchoring motifs, target Y. pseudotuberculosis invasin to the cell surface of the bacterium. Since this strain of L. plantarum can change the immunological tolerance of an immune response, it can be used to increase antigen immunogenicity and as a delivery vehicle for mucosal vaccines [18].

Lastly, strains of L. plantarum can be used to stimulate production of antibodies. A study used L. plantarum that expressed Borrelia burgdorferi (B. burgdorferi) OspA lipoprotein and found that it stimulated the production of OspA-specific IgA and IgG antibodies as wells as pro- and anti-inflammatory cytokines (Figure 7) [19]. It did not lead to secretion of cytokine (IL-8) by epithelial cells and did not induce inflammatory effects. The recombinant L. plantarum is capable of stimulating a protective immune response via T-cell (Th1/Th2) mediated immunity.

Conclusion

L. plantarum is a bacterium that is very versatile and can adapt to various environmental conditions since it can ferment different types of carbohydrates and sugars. Specifically, it is able to withstand and grow in the harsh conditions of the gastrointestinal tract. Because of this, it can be used as a probiotic to benefit human health. A variety of strains or recombinants of L. plantarum can aid in restoring the homeostasis of the flora in the intestines, limit the amount of pathogenic bacteria, and could be potentially used as a vehicles for vaccines. However, not all the mechanisms are known for how L. plantarum can be used as in a vaccine, so many research groups are trying to answer this question. Within the next few years, it is expected that more developments will be made to utilize the probiotic property of L. plantarum to benefit human health.

References

1. Kleerebezem, M.; Boekhorst, J.; van Kranenburg, R.; Molenaar, D.; Kuipers, O. P.; Leer, R.; Tarchini, R.; Peters, S.A.; Sandbrink, H.M.; Fiers, M.W.E.J.; Stiekema, W.; Lankhorst, R.M.K.; Bron, P.A.; Hoffer, S.M.; Groot, M.N.N.; Kerkhoven, R.; de Vries, M.; Ursing, B.; de Vos, W.M.; Siezen, R.J. Complete genome sequence of Lactobacillus plantarum WCFS1. PNAS 2003, 100, 1990-1995.

2. Quatravaux, S.; Remize, F.; Bryckaert, E.; Colavizza, D.; Guzzo, J. Examination of Lactobacillus plantarum lactate metabolism side effects in relation to the modulation of aeration parameters. J Appl. Microbiol. 2006, 101, 903-912.

3.Siezen, R.J.; van Hylckama Vlieg, J.E. Genomic diversity and versatility of Lactobacillus plantarum, a natural metabolic engineer. Microbial Cell Factories 2011, 10, 1-13.

4. Christensen, J.; Dudley, E.; Pederson, J.; Steele, J. Peptidases and Amino Acid Catabolism in Lactic Acid Bacteria. Antonie Van Leeuwenhoek 1999, 76, 217-46.

5. Jockers, David. "Learn about the Importance of Good Bacteria, Part II: Lactobacillus Plantarum." NaturalNews. NaturalNews, 1 Jan. 2010. Web. 19 Apr. 2013.

6. Sunanliganon, C.; Thong-Ngam, D.; Tumwasorn, S.; Klaikeaw, N. Lactobacillus plantarum B7 inhibits Helicobacter pylori growth and attenuates gastric inflammation. World J Gastroenterol. 2012, 18, 2472-2480.

7. Beganovic, J.; Pavunc, A.L.; Gjuracic, K.; Spoljarec, M.; Suskovic, J.; Kos, B. Improved sauerkraut production with probiotic strain Lactobacillus plantarum L4 and Leuconostoc mesenteroides LMG 7954. J. Food Sci. 2011, 76, M124-M129.

8. Crowley, S.; Mahony, J.; van Sinderen, D. Comparative analysis of two antifungal Lactobacillus plantarum isolates and their application as bioprotectants in refrigerated foods. J. Appl. Microbiol. 2012, 113, 1417-1427. 9. "Probiotic." Merriam-Webster. Merriam-Webster, 2013. Web. 22 Apr. 2013.

10. Bokhorst-van de Veen, H.; Lee, I.; Marco, M.L.; Wels, M.; Bron, P.A.; Kleerebezem, M. Modulation of Lactobacillus plantarum gastrointestinal robustness by fermentation conditions enables identification of bacterial robustness markers. PLoS ONE 2012, 7, e39052.

11. Cebeci, A.; Gurakan, C. Properties of potential probiotic Lactobacillus plantarum strains. Food Microbiology 2003, 20, 511-518.

12. Adlerberth, I.; Ahrne, S.; Johansson, M.L.; Molin, G.; Hanson, L.A.; Wold, A.E. A mannose-specific adherence mechanism in Lactobacillus plantarum conferring binding to the human colonic cell line HT-29. Appl Environ. Microbiol. 1996, 62, 2244-2251.

13. Duary, R.K.; Bhausaheb, M.A.; Batish, V.K.; Grover, S. Anti-inflammatory and iummnomodulatory efficacy of indigenous probiotic Lactobacillus plantarum Lp91 in colitis mouse model. Mol Biol Rep. 2012, 39, 4765-4775.

14. Liu, Z.; Zhang, P.; Ma, Y.; Chen, H.; Zhou, Y.; Zhang, M.; Chu, Z.; Qin, H. Lactobacillus plantarum prevents the development of colitis in IL-10-deficient mouse by reducing the intestinal permeability. Mol. Biol. Rep. 2011, 38, 1353-1361.

15. Ko, J.S.; Yang, H.R.; Chang, J.Y.; Seo, J.K. Lactobacillus plantarum inhibits epithelial dysfunction and interleukin-8 secretion induced by tumor necrosis factor-α. World J. Gastroenterol. 2007, 13, 1962-1965.

16. Zhang, M.; Wang, X.; Zhou, Y.; Ma, Y.; Shen, T.; Chen, H.; Chu, Z.; Qin, H. Effects of oral Lactobacillus plantarum on hepatocyte tight junction structure and function in rats with obstructive jaundice. Mol. Biol. Rep. 2010, 37, 2989-2999.

17. Daniel, C.; Roussel, Y.; Kleerebezem, M.; Pot, B. Recombinant lactic acid bacteria as mucosal biotherapeutic agents. Trends Biotechnol. 2011, 29, 499- 508.

18. Fredriksen, L.; Kleiveland, C.R.; Hut, L.T.O.; Nygaard, C.S.; Eijsink, V.G.H.; Mathiesen, G. Surface display of N-terminally anchored invasin by Lactobacillus plantarum activates NF-κB in monocytes. Appl. Environ. Microbiol. 2012, 78, 5864-5871.

19. Rio, B.; Seegers, J.F.M.L.; Gomes-Solecki, M. Immune response of Lactobacillus plantarum expressing Borrelia burgdorferi OspA is modulated by the lipid modification of the antigen. PLoS ONE 2010, 5, e11199.

20. Rigaux, P.; Daniel, C.; Hisbergues, M.; Muraille, E.; Hols, P.; Pot, B.; Pestel, J.; Jacquet, A. Immunomodulatory properties of Lactobacillus plantarum and its use as a recombinant vaccine against mite allergy. Allergy 2009, 64, 406-414.

Edited by student of Joan Slonczewski for BIOL 238 Microbiology, 2013, Kenyon College.