Zinc Finger Nuclease (ZFN): Difference between revisions
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==History== | ==History== | ||
While other gene targeting procedures have existed for quite some time, the success of earlier methods were dependent on several factors including homologous recombination. Also, while genetic changes were observed in fungi like ''Saccharomyces cerevisiae'' and bacteria, most methods did not work on eukaryotic organisms. However, when studies in the 80s revealed that double-stranded DNA breaks greatly increased the frequency of recombination and thus successful genetic modifiactions, tools such as ZFNs became favored (Rudin et. al.). | While other gene targeting procedures have existed for quite some time, the success of earlier methods were dependent on several factors including homologous recombination. Also, while genetic changes were observed in fungi like ''Saccharomyces cerevisiae'' and bacteria, most methods did not work on eukaryotic organisms. However, when studies in the 80s revealed that double-stranded DNA breaks greatly increased the frequency of recombination and thus successful genetic modifiactions, tools such as ZFNs became favored. Previously, stem cells were required (Rudin et. al.). | ||
The insertion code consists of: | The insertion code consists of: | ||
Revision as of 07:53, 5 December 2013
Introduction
Zinc finger nucleases (ZFNs)are enzymes which can be used to cleave DNA at select points, makes them useful tools for genetic engineering. ZFNs are composed of a DNA binding-domain and a DNA cleaving-domain.
History
While other gene targeting procedures have existed for quite some time, the success of earlier methods were dependent on several factors including homologous recombination. Also, while genetic changes were observed in fungi like Saccharomyces cerevisiae and bacteria, most methods did not work on eukaryotic organisms. However, when studies in the 80s revealed that double-stranded DNA breaks greatly increased the frequency of recombination and thus successful genetic modifiactions, tools such as ZFNs became favored. Previously, stem cells were required (Rudin et. al.).
The insertion code consists of:
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Filename: PHIL_1181_lores.jpg
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Legend/credit: Diagram of a zinc finger nuclease. The zinc fingers are part of the binding-domain and have precisely identified and bound to a group of matching nucleobases in the DNA sequence. The cleaving-domains are Fok 1, which make a break in the DNA in both strands. Image credit to Stewart, C. N. and Burris, J. Jr.
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Subscript: H2O
Superscript: Fe3+
Section 1
Include some current research in each topic, with at least one figure showing data.
Section 2
Include some current research in each topic, with at least one figure showing data.
Section 3
Include some current research in each topic, with at least one figure showing data.
Conclusion
Overall paper length should be 3,000 words, with at least 3 figures.
