Brevetoxin

Structure of brevetoxin, types A and B, produced by dinoflagellate K. brevis[1]

Brevetoxin is a kind of neurotoxin produced by Karenia brevis. [3] K. brevis is a species of dinoflagellate, a group of the Kingdom Protista. They are a kind of algae, which means they are subject to the occurence of algal blooms, in which the ordinarily less noticeable populations of algae explode to much more noticeable levels. It is at these levels that K. brevis begins to pose a problem, as the brevetoxin it produces reaches concentrations high enough to harm humans, whether through direct contact, consumption of contaminated shellfish, or inhalation of the toxin carried through the air.

Karenia brevis

Although a number of Karenia species have been described as of yet, K. brevis, the main producer of brevetoxin, occurs primarily in the Gulf of Mexico, particularly on the Western Gulf coast of Florida. It occurs in other parts of the Gulf as well, as far west as the Texas coast. K. brevis occurence outside the Gulf of Mexico is uncommon, but it infrequently occurs in the waters of the Indian River Lagoon on the Atlantic coast of Florida. A bloom in 2007-2008 was enough to bring K. brevis levels in the lagoon high enough to make the shellfish in the area dangerous for consumption. Algal blooms can occur at virtually any time of year, but their occurrence seems to be increased between the months of September and February.[4]

Physiological reasons for production of brevetoxin by K. brevis is subject to research and debate. One study conducted measured whether brevetoxin production responded to changes in water salinity, determining that intracellular concentration of brevetoxin increased from 10% to 53% within five days of a water salinity change from 35 to 27. The lowered water salinity would make it difficult for K. brevis to maintain intracellular sodium concentrations due to hypoosmotic stress, as sodium flows out of the cell, or water flows in, in a natural attempt to maintain a concentration balance between the cell and the surrounding environment.[5] The function of brevetoxin is to attach to sodium channels in cell membranes, forcing the sodium channels open to allow for influx of sodium ions.[6] In the dinoflagellates, this could serve as a physiological mechanism to help maintain sodium levels inside the cell when water salinity changes. However, it is not definitively known to function as such within the dinoflagellates. Scientists have suggested brevetoxin's function to lie also in utilization as an allelopathic chemical, whether to give the organism a competitive edge over other species of phytoplankton, or against organisms which may feed on the plankton.

Affliction and Symptoms

The amount of toxin produced in small numbers of these K. brevis is typically not dangerous. However, when they reproduce en masse, as in the case of the red tide algal blooms, enough brevetoxin is generated to be considered dangerous to humans, as well as other kinds of life. Wildlife is most directly affected, specifically those species which depend on or live in the ocean, such as fish, water fowl, sea turtles and dolphins. Animals that largely depend on affected animals as a food source, such a coastal raptors may not directly contact the water enough to feel the effects of the toxin directly. However, they are also affected due to the decrease in uncontaminated or fresh food sources. Humans tend to be intelligent enough not to swim directly in the red tides, although contact of brevetoxin to skin can lead to toxic effects. Instead, humans are most often exposed to brevetoxin through consumption of shellfish contaminated with the chemical. In rare cases, inhalation is possible if brevetoxins are swept into the air by ocean surf or onshore winds.[7]

K. brevis, responsible for brevetoxin production. Location on page subject to change.[2]

Illness onset following consumption of contaminated shellfish is known as Neurotoxic Shellfish Poisoning, or NSP, wherein brevetoxins will proceed to affect the gastro-intestinal (GI) tract as well as the neurological system. GI tract related symptoms include vomiting, diarrhea and pain in the abdomen. Neurological symptoms consist of paresthesia (a pricking, tingling sensation), reversal of perception of hot and cold temperature sensation, vertigo (false sense of motion), and ataxia (involuntary muscular movement, or spasms). Inhalation of brevetoxin may result in coughing, dyspnea (shortness of breath), and bronchial spasms. [8] No human fatalities have yet been attributed to NSP, but the concentrations of brevetoxins produced in annual algal blooms are responsible for significant annual animal fatalities along the Gulf of Mexico and the south/mid-Atlantic coast. [9]

In innervated organisms, brevetoxin causes problems with the basic functioning mechanism of nerves. Nerves operate through changes in electrical charge through sodium-potassium pumps. Ordinarily, these pumps maintain a slight negative charge inside the cell relative to the outside. The nerve cell fires by allowing sodium ions into the cell to increase the relative charge, generating electricity and creating an action potential. Brevetoxin binds to the sodium channels and allows for unmitigated flow of sodium ions into nerve cells. This results in the general loss of fine nervous control, evidenced by the paresthesia, ataxia, and other symptoms.

Conclusion

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References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

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Edited by [Author Name], student of Joan Slonczewski for BIOL 116 Information in Living Systems, 2013, Kenyon College.