Bacteroide composition in the gut

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Bacteria have played a significant role in Homo sapiens evolution. Only ten percent of the cells existing in the body are human. Genetically, we are about 1% human, and 99% bacterial [10.

Figure 1. Bacteroides thetaiotaomicron in relation to food particles in the mouse intestine, provided by Larsson et al., 2012 [7

The human colon contains the majority of microorganisms in the body, and 25% of these are species of Bacteroides. Bacteroides species are anaerobic, non-spore forming, gram-negative rods that have adapted to, and now thrive in, the human gut [8]. The relationship between Bacteroides and the host has recently been considered mutual, given that the relationship increases the fitness of both species. Given the long history of coevolution between microbiota and the intestine, it has now been shown that bacteroides function as a multifunctional organ that provides metabolic components we do contain within our own genome [14]. Some of these metabolic traits include carbohydrate fermentation. Digesting complex polysaccharides frees short peptides to be reabsorbed by the large intestine and to be used for energy by the host. Through various metabolic mechanisms, Bacteroides provide amino acids and vitamins, while simultaneously utilizing a wide range of dietary polysaccharides for growth [6].

Due to the metabolic role of Bacteroides in carbohydrate fermentation, there has been some evidence showing a relationship between diet, gut flora, and obesity. However, causation of these variables is still up for debate. Given the significant role Bacteroides play in the gut, the effect of diet on their mechanisms of action and composition overall is a relevant topic of analysis [10]. How body flora composition might affect adiposity is particularly crucial in light of the drastic shift in food source in the United States and its consequent growing obesity epidemic.

Characteristics of the Gut Microbiota

Figure 2. Lateral Gene Transfer in Bacteroidetes Promotes Niche Specialization(A) Proportions of genes found in each genome. Non-laterally transferred genes (blue) are proportionally more often involved in metabolism. Genes assigned “Primary metabolism” (yellow) and “Protein biosynthesis” (red), are compared. Upward-pointing arrowhead indicate gene enrichment and downward-pointing arrowhead show gene depletion . Asterisks indicate statistical significance, single asterisk (*) indicates p < 0.05; double asterisks (**) indicate p < 0.01; and triple asterisks (***) indicate p < 0.001. For B,C, and D see original publication by Xu et al., 2007[13

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Characterizing the Proteome and the Genome

Recently, genomic and proteomic analyses have provided insight into the mechanisms in which microbiota adapt and thrive within varying microenvironments. Sequencing B. thetaiotaomicron and B. fragilis show notable genomic commonalities with eukayotes that allow these species to adapt. These Bacteroides, like eukaryotes, have an array of possibilities for gene expression [13]. A large portion of the their genetic make-up consists of proteins containing over 1,000 amino acids, which, it seems, allow for a vast selection of relevant proteins for selective functions.

Adaptive Characteristics

The adaptive characteristics to specific niches within the gut are extremeley variable and have been shown through comparisons to other known hologous proteins and by camparing Bacteroides to other non-gut microbiota. The specific traits identified by both these means are discussed below.

In order to characterize the various functions of the Bacteroide genome, putative relevant proteins have been determined by homology to other known proteins. Of the 4779 proteins identified in the B. thetaiotaomicron proteome, 58% were ascribed a supposed function based on other known proteins, 18% were homologous to proteins without a known function, and 24% were not homologous to any protein identified in the public domain [13]. The variety of functions is presumed to aid Bacteroides species in multiple ways: the proteome induces the mechanism by which some Bacteroides acquire and hydrolyze otherwise indigestible dietary polysaccharides, and includes an environment-sensing apparatus with extracytoplasmic function sigma factors and one- and two-component signal transduction systems [13]

B. thetaiotaomicron contains the capacity to utilize glycans derived from the colon. The majority (61%) of the necessary enzymes (glycohyrolases) potentially exist in the periplasm, and are necessary to the polysaccharide fermentation process that increases energy availability to the host by freeing fatty acids to be reabsorbed by the intestine. Other extracellular proteins have been shown to facilitate the binding of starches to the cell surface to be digested by other extracellular enzymes [13].

Additionally, B. thetaiotaomicron was shown to contain a significant population of ECF-sigma factors, which according to other identified homologous genes, are cotranscribed with regulatory proteins that respond to external environmental stimulus and act on gene expression accordingly [13].

The majority of one-component signal transduction systems were shown to be similar to other genes responsible for nutrient utilization, providing further evidence of Bacteroides’ genetically evolved fitness advantage in nutrient acquisition[13].

Bacteroide characteristics found in comparisons with non-gut bacteria:

Other studies have confirmed and contributed to the understanding of Bacteroide adaptive traits and functionality through comparisons with non-gut microbiota. Xu and associates [15] sequenced the genomes of two gut-dwelling Bacteroide species Bacteroides vulgatus and Bacteroides distasonis andcompared their common orthologs to non-gut Bacteroides Porphyromonas gingivalis W83 (found in human mouth) and Cytophaga hutchinsonii ATCC 33406 (found in soil) (Figure 2).

It was shown that factors such as lateral gene transfer, mobile elements, and gene amplification allow Bacteroides to vary the surface of their cells, sense the environment, and gain access to nutrients in the intestine (Figure 2). The authors concluded that these processes have allowed the symbiotic relationship between Bacteroidetes and the human gut environment to thrive.

Summary:

To conclude, through genomic and proteomic sequencing, some of the mechanism underlying the adaptive capabilities of the Bacteroide species in the human gut have been identified. The expansive array of proteins available to them mediates their ability to respond to their environment, thrive within the rapidly changing microenvironment of the colon, and acquire and ferment complex carbohydrates for their own energy source as well as for the host [13]. In studying how various factors (i.e. diet, stress, or antibiotic consumption) affect the microbiome, the adaptational activity of Bacteroidetes can contribute to the understanding of what cellular processes are specifically affected by external factors affecting the composition of gut flora [15].

Changes in Microbiota Compostion Associated with Diet

Understanding the taxonomy and identification of the microbiota in the gut has increased substantially in recent years. The study of enterotypes have contributed substantially to our understanding of the microbiota. We each have specific compositions of bacteria living with us, and it seems what we eat and where we are from hold significant roles in determining the species that live within us! Additionally, these findings can help us understand why certain populations obtain ailments that others do not. The answer could be in population-specific enterotypes.

An Overview of Enterotypes

In 2011, Arumugam and colleagues identified three clusters of microbiota species, termed enterotypes, defined as “densely populated areas in a multi-dimensional space of community composition” (p. 177) [1]. Type 1 is characterized by high levels of Bacteroides, type two is dominated by Prevotella, and Ruminococcus is prevalent in type 3. In accordance with other genomic and proteomic analysis, the Bacteroides and Parabacteroides found in the type 1 enterotype fermented carbohydrates and proteins by the use of galactosidases, hexosaminidases, and proteases enzymes for degradation as well as through glycolysis and pentose phosphate pathways. It was shown that enterotype composition is species driven, however, identification of molecular functioning of each species is necessary to determine health differences between hosts with varying enterotypes. Age, body mass index, gender, or nationality showed no clear causation to enterotype composition.

Figure 3. (A) Bacterial taxon for 15 most abundant types in human stool data and pooled sewage data. (B) Box plot showing six most abundant bacterial families in human stool data both from human stool data and sewage stool data sets when non-human oligotypes were removed. Circles represent mean values, and vertical lines show first and third quartiles. Source: Newton et al., 2015 [3

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Another study founded categorical bacterial families among different U.S. populations. The study found sewage samples to reflect the bacterial composition of human stool samples in the United States (Figure 3). While the representation of Lachnospiraceae, Prevotellaceae, and Bacteroidaceae seemed to differ between sewage and human stool data, the oligotype relative abundance profiles were similar. Furthermore, the results reflected thos of Arumugam and associates, showing similar U.S. specific bacterial composition characterized by enterotype 1[3]].

To reiterate, several studies have confirmed differences in types of bacteria in the gut from people of various geographic locations.

Dietary Changes and Enterotype Composition

Whether or not diet is the ultimate causative factor in determining enterotype differences is not yet clear. However, some studies show long-term dietary changes alter the microbiota of the gut. A study by Wu and colleagues (2011) analyzed fecal samples, using diet inventories and 16S rDNA sequencing, from 98 individuals in order to determine the effect of diet on enterotype identity and microbiome composition [14]. The authors concluded that while microbiome composition changed within 24 hours, enterotype identity shifts are associated with long-term dietary changes. Composition of enterotypes, particularly protein and animal fat (Bacteroides) versus carbohydrates (Prevotella), were most affected in long-term analysis. Their findings provide evidence that diet affects the amount of bacteroides living in the gut, and that the change is time-dependent.

Another study also showed a possible correlation between long-term diet and enterotype composition[4]. De Filippo et al., in 2010, used high-throughput 16S rDNA sequencing and biochemical analyses to assess microbiota differences between children eating a fiber-rich, high-carbohydrate diet in Burkina Faso and European children fed diets high in fat and protein. The European children showed high levels of Bacteroides, while the Burkina Faso children contained microbiomes enriched by the Prevotella enterotype, known to contain genes that allow for xylan and cellulose hydrolysis. This genetic advantage may be completely lacking in children fed western diets. While there are many distinguishing factors between the two populations that might account for enterotype differences, diet is an obvious possible causative factor given what is known about the metabolic properties of bacterial species in the gut.

Possible Associations Between Enterotype and Disease

The authors of the Africa/Europe comparison hypothesize that diseases associated with enterotype composition may be treated by long-term dietary changes. It is also suggested that preservation of human communities in isolated areas is important, in that they may contain fitness advantages due to ancient coevolutionary symbioses between themselves and their microbiomes. These fitness advantages may explain why these communities are resistant to certain diseases [4].

Bacteroides and Obesity

Microbiota May Influence BMI

Several studies have shown relationships between diet, obesity, and microbiome composition. One study analyzed via genetic sequencing of the bacterial contents of fecal samples from 12 “obese” and 5 “lean” (according to the BMI scale) individuals [7]. Obese individuals were found to have 20% more Firmicutes species and almost 90% less Bacteroidetes compared to the lean group. Furthermore, after one year of spent on a low-carbohydrate or low-fat diet, obese individuals showed a significant change in microbiota composition after shedding 25% of their body weight. Proportions of Bacteriodetes increased while the Firmicutes population decreased after the year-long diet and weight loss (Fig. 4). The results by Ley and associates provide evidence toward an inverse relationship between adiposity and Bacteroides proportions in the gut. Their evidence also suggests dietary therapeutic techniques toward treating or preventing obesity-associated disease. However, the small sample size of the study lens itself to critical debate in its applicability to the larger population.

Figure 4. Principal correspondence analysis of UniFrac distances. Difference in gut microbiota according to (A) geographic location, (B) BMI, (C) Gender. Source: Ecobar et al., 2014[6

A study by Turnbaugh (2009) [10] showed parallel results to those of Ley, Turnbaugh, and Gordon (2006)[7]The authors successfully mimicked human gut environments in mice by transplanting fecal matter into germ-free C57BL/6J (“humanized”) mice by metagenomic analysis of their bacterial colonization. Transitioning from a low-fat, plant-rich diet to a high-sugar “western” diet significantly changed the contents of the host’s microbiota in a single day, and these changes altered metabolic pathways and gene expression of the microbiome, and eventually increased adiposity. Adiposity fluctuated depending on microbiota transplantation. Humanized mice fed the western diet showed higher levels of Firmicute species and Erysipelotrichi and Bacilli species and less representation of Bacteroides (Fig 4). The pathways identified in the microbiome of the lean mice included glycan degradation and fatty acid metabolism, the same pathways previously assigned to Bacteroides. These findings support the hypothesis that Bacteroides may contribute to lower body mass, and that microbiota composition in the colon is responsive to changes in diet.

Possible Causation

Some empirical evidence suggests high-fat diets and their effects on microbiota composition negatively impact gut permeability, leading to intestinal inflammation through translocation of Tlr ligands. Cani’s data suggests increased plasma LPS induces inflammation, which was shown to contribute to weight gain. During the inflammation process, the proportion of Bacteroides increased, providing one explanatory factor for diet-induced shifts in microbiota composition [2].

BMI and Bacteroid Composition May be Unrelated

A correlation between decreased proportions of Bacteroides in the microbiota and obesity has not been found in every investigation on the topic. Arumugam and colleagues showed no correlation between type 1 enterotype and leanness [1]. SImilarly, Duncan and colleagues found no evidence of differences in Bacteroide proportions in fecal samples between obese and non-obese subjects, and weight loss dieting did not alter their original observations [4].

Geography Influences Microbiota Composition

In a study by Escobar and colleagues, geography play an important role in determining microbiota composition. Through rDNA pyrosequencing of the gut microbiota of Columbian adults and comparing it with results from Americans, Europeans, and Japanese and South Koreans, they found that geography indicated more variance in microbiota composition than BMI or gender (Figure 4). They also found that Firmicutes presence was lessened with increasing BMI, however, they found no change in levels of Bacteroidetes. The same result was found in Americans, while the Japanese showed a higher proportion of Actinobacteria.

These results show that factors contributing to proportions of Bacteroide composition in the gut are complex. Indeed, geography, diet, and body mass have each been shown independently to have an effect, however, many of the results negate previous findings. More research should be conducted to accumulate evidence for some of these hypotheses.

Further Reading

Study of functions of Bacteroides has expanded dramatically in recent years. Bacteroide fragilis has been found to regulate cell surface adaptation through DNA inversions, help regulate the immune system, and modulate inflammation, to name a few. It seems that we can no longer study the gut, or the whole body for that matter, without also studying its microbial communities. They have evolved with us since our origins, therefore we cannot think of ourselves in isolation. Furthermore, our actions, birthplace, and ecosystem affect these communities. Perhaps an understanding of them will reveal how our lifestyles can benefit the commensals that benefit us. Below are some relevant articles worth exploring for more information.

Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation--Proceedings of the National Academy of Sciences

Microbes, immunoregulation, and the gut--British Society of Gastroentemology

Origins and evolution of the Western diet: health implications for the 21st century--American Society for Clinical Nutrition

in inflammatory bowel disease: is it all gut flora modulation?--British Society of Gastroentemology

References

1. Arumugam M, Raes J, Pelletier E, et al. Enterotypes of the human gut microbiome. Nature. 2011;473(7346):174-180. doi:10.1038/nature09944.

2. Cani, Patrice D., and Nathalie M. Delzenne. "The Role of the Gut Microbiota in Energy Metabolism and Metabolic Disease." Current Pharmaceutical Design 15.13 (2009): 1546-558. Web.

3. JS, Klotz B, Valdes BE, Agudelo GM. The gut microbiota of Colombians differs from that of Americans, Europeans and Asians. BMC Microbiol. 2014;14(1):311

4. De Filippo C, Cavalieri D, Di Paola M, et al. Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa. Proceedings of the National Academy of Sciences of the United States of America. 2010;107(33):14691-14696.

5. Duncan S, Lobley G, Flint H, et al. Human colonic microbiota associated with diet, obesity and weight loss. International Journal Of Obesity. November 2008;32(11):1720-1724. Available from: Academic Search Premier, Ipswich, MA. Accessed March 21, 2015.

6. Hooper LV, Midtvedt T, Gordon JI. How host-microbial interactions shape the nutrient environment of the mammalian intestine. Annu Rev Nutr. 2002;22:283-307.

7. Larsson, E., V. Tremaroli, Y. S. Lee, O. Koren, I. Nookaew, A. Fricker, J. Nielsen, R. E. Ley, and F. Backhed. "Analysis of Gut Microbial Regulation of Host Gene Expression along the Length of the Gut and Regulation of Gut Microbial Ecology through MyD88." Gut 61.8 (2012): 1124-131. Gut. BMJ, 3 July 2012. Web. 20 Mar. 2015.

8. Ley RE, Turnbaugh PJ, Klein S, Gordon JI. Microbial ecology: human gut microbes associated with obesity. Nature. 2006;444(7122):1022-3.

9. RJ, Mclellan SL, Dila DK, et al. Sewage reflects the microbiomes of human populations. MBio. 2015;6(2):e02574.

10. Turnbaugh PJ, Ridaura VK, Faith JJ, Rey FE, Knight R, Gordon JI. The Effect of Diet on the Human Gut Microbiome: A Metagenomic Analysis in Humanized Gnotobiotic Mice. Science translational medicine. 2009;1(6):6ra14.

11. Wexler HM. Bacteroides: the Good, the Bad, and the Nitty-Gritty. Clinical Microbiology Reviews. 2007;20(4):593-621. doi:10.1128/CMR.00008-07.

12. Wu GD, Chen J, Hoffmann C, et al. Linking Long-Term Dietary Patterns with Gut Microbial Enterotypes. Science (New York, N.y). 2011;334(6052):105-108. doi:10.1126/science.1208344.

13. Xu J, Bjursell MK, Himrod J, et al. A genomic view of the human-Bacteroides thetaiotaomicron symbiosis. Science. 2003;299(5615):2074-6.

14. Xu J, Gordon JI. Honor thy symbionts. Proceedings of the National Academy of Sciences of the United States of America. 2003;100(18):10452-10459.

15. Xu J, Mahowald MA, Ley RE, et al. Evolution of symbiotic bacteria in the distal human intestine. PLoS Biol. 2007;5(7):e156.

Edited by Maggie Schein, a student of Suzanne Kern in BIOL168L S2 (Microbiology) in The Keck Science Department of the Claremont Colleges Spring 2014.