Tina Torres Bacillus thuringiensis: Difference between revisions
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==Cell Structure, Metabolism and Life Cycle== | ==Cell Structure, Metabolism and Life Cycle== | ||
This gram positive microorganism has a thick, cross linked peptidoglycan layer in the bacterial cell wall. It is harmful to many insects, it works by | |||
Interesting features of cell structure; how it gains energy; what important molecules it produces. | Interesting features of cell structure; how it gains energy; what important molecules it produces. | ||
==Physiology and Pathogenesis== | ==Physiology and Pathogenesis== | ||
Revision as of 13:15, 8 May 2015
Classification
Domain: Bacteria, Phylum: Firmicutes, Class: Bacilli, Order: Bacillales, Family: Bacillaceae [Others may be used. Use NCBI link to find]
Species
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NCBI: Taxonomy |
Bacillus thuringiensis
Habitat Information
This soil sample was collected at 289 Spring Lane, Dripping Springs, TX 78620 Temperature: 60 F Humidity: 40% Wind Speed: NE 14G 22 mph Dewpoint: 35 F GPS coordinates:
Description and Significance
When streaked on an LB plate, the colonies that formed were opaque in appearance and flat. When tested for antimicrobial properties the one antibiotic that showed the most susceptibility was Sulfisoxazole, also a small zone of inhibition was seen with Ampicillin. Linezolid and Cefamandole showed no zone of inhibition.
Bacillus thuringiensis is a gram positive, soil dwelling bacterium that is commonly used as a biological pesticide.
[File:LB_Streak_Plate.jpg]
Genome Structure
This is the forward sequence I used to determine that my soil sample was Bacillus thuringiensis:
GACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGAGACTTGAGTGCAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGAGGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTANTGCTGAAGTTAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCNNAGGAATTGACNGGGGCCCGCACAANCGGTGGANCATGTGGTTTAATT ACCAGGTNTTGAAATCCTCTGANAACCCTANAGATACGGCNTCTCNCNTCTNNAACATANTGAC
This organism is gram positive and form endospores.
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.
Cell Structure, Metabolism and Life Cycle
This gram positive microorganism has a thick, cross linked peptidoglycan layer in the bacterial cell wall. It is harmful to many insects, it works by
Interesting features of cell structure; how it gains energy; what important molecules it produces.
Physiology and Pathogenesis
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.
References
Author
Page authored by _____, student of Prof. Kristine Hollingsworth at Austin Community College.
