Borrelia burgdorferi

A Microbial Biorealm page on the genus Borrelia burgdorferi

Classification

Higher order taxa

Bacteria; Spirochaetes (Phylum); Spirochaetes (Class); Spirochaetales; Spirochaetaceae

Species

Borrelia burgdorferi

NCBI: Taxonomy

Description and significance

Borrelia burgdorferi is a spirochete bacteria that is well known as the causative agent of Lyme disease. In 1982, a few years after Lyme disease was first diagnosed, it was determined that B. burgdorferi was being transmitted to humans by ticks. Since then, it's genome has been fully sequenced and researchers have been able to study the interesting mechanism of host and vector transmission that the bacteria has adopted. However, the exact mechanisms for it's pathology are still trying to be understood. B. burgdorferi is currently being used to answer interesting questions surrounding vector transmission, host adaptation and plasmid maintenance. According to the Center for Disease Control, Lyme disease caused by B. burgdorferi has become the most common vector-borne bacterial disease in the world. B. burgdorferi's unique structure and adaptations allow it to be an effective invasive pathogen and an important organism to research.

Borrelia burgdorferi bacterium, CDC

Describe the appearance, habitat, etc. of the organism, and why it is important enough to have its genome sequenced. Describe how and where it was isolated. Include a picture or two (with sources) if you can find them.

Genome structure

B. burgdorferi has a very unique genome. It consists of one large linear chromosome that is 910,725 base pairs long. This chromosome contains approximately 853 genes coding for basic functions such as DNA replication, transcription, translation as well as transport and metabolic systems. However, in addition to this chromosome, the bacterium also contains 21 other linear and circular plasmids that add an additional 533,000 base pairs of DNA. The genomic organization of B. burgdorferi is unique due to the high number of plasmids. It has been seen that some bacteria lacking a complete set of plasmids are unable to successfully infect their host, leading researchers to believe that the plasmids may encode virulent DNA. However, the sequenced genome does not contain any obvious genes coding for pathogenesis, and therefore, the mechanisms of B. burgdorferi infections are still a hot topic of research (Fraser).

Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Does it have any plasmids? Are they important to the organism's lifestyle?

Cell structure and metabolism

B. burgdorferi is a helical shaped spirochete bacterium. It has an inner and outer membrane as well as a flexible cell wall. Inside the bacteria's cell membranes is the protoplasmic cylinder. The cell is normally only 1 μm wide but can be 10-25 μm long. Spirochete bacteria such as B. burgdorferi have a unique structural characteristic. The cell's flagella are located inside the periplasm between the inner and outer cell membranes. The interactions between the flagella and cell cylinder allows the cell to travel through highly viscous fluids and materials (Charon). This adaptation is especially critical for the ability of B. burgdorferi to travel through the tissue of it's infected host or vector, causing it to be highly invasive.

Another important cellular component of B. burgdorferi is it's outer surface proteins. Researchers have investigated a variety of outer membrane surface proteins (Osp) and have found them to play important roles in transmission of the bacteria. The cell expresses different surface proteins, most notably OspA and OspC. However, they are not seen on all cells. When the bacteria is inside of an unfed tick, it produces mostly OspA. However, the bacteria that are inside of infected mammals express mostly OspC. These, and other, outer surface proteins may play important roles in the ability of the bacteria to infect and survive inside their hosts.

Describe any interesting features and/or cell structures; how it gains energy; what important molecules it produces.

Ecology

Borrelia burgdorferi is maintained in a natural cycle of infection by ticks. The ticks acquire and transmit the bacteria by feeding on a variety of small mammals and birds that act as a reservoir host for the Borrelia. B. burgdorferi is transmitted specifically by ticks of the genus Ixodes, which include a variety of different species found in different geographical locations. Ixodes scapularis is the most common vector and is found in the Northeastern US. Ixodes pacificus is found on the west coast, while species Ixodes ricinus and persulcatus are found in Europe and Asia. These ticks acquire and transmit the bacteria during three distinct feeding phases of their life cycle; larval, nymphal and adult stages.

Describe any interactions with other organisms (included eukaryotes), contributions to the environment, effect on environment, etc.

Pathology

Lyme disease was first identified due to an outbreak of juvenile rheumatoid arthritis. This is one of the later stage symptoms of the B. burgdorferi infection. Ticks use many small mammals to harbor the bacteria, and humans only become inadvertently infected when bitten. The first sign of infection however is the Erythema migrans, a circular rash at the site of the tick bite after a few days delay. From their, infected humans may develop similar rashes on other sites of the body as well as symptoms such as fatigue, fever, headache, and muscle and joint aches. If the infection spreads throughout the body it has reached what is known as the Stage II infection and can cause symptoms such as facial palsy, meningitis, extreme joint pain and heart palpitations. If it is left untreated for a few months the infection will reach Stage III and patients may develop arthritis as well as severe joint pain and swelling.

Erythema migrans, Ross Ritter, P.A.

How does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.

Application to Biotechnology

Does this organism produce any useful compounds or enzymes? What are they and how are they used?

Current Research

Much of the current research surrounding B. burgdorferi concentrates on discovering the mechanisms through which the bacteria invades the human body in order to produce more effective treatments against it. As a member of the Spirocheate phylum, B. burgdorferi is quite distinct from many other bacterial groups and therefore many of the common genetic tools do not work on the bacteria. It is also notoriously difficult to transform.

Enter summaries of the most recent research here--at least three required

References

Charon, NW., Greenberg, EP., Koopman, MBH., and Limberger RJ. "Spirochete chemotaxis, motility, and the structure of the spirochetal periplasmic flagella." Research in Microbiology. 1992. 143(6). p. 97-603.

Fraser, CM., Casjens, S., Huang, WM., Sutton, GG., Clayton, R. et al. "Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi." Nature. 1997 Dec 11;390(6660):580-6.

Motaleb, MA. et al. "Borrelia burgdorferi periplasmic flagella have both skeletal and motility functions." Proceedings of the National Academy of Sciences of the USA. 2000 Sept 26. Volume 97(20). p. 10899−904.

Rosa, PA., Tilly, K., and Stewart, PE. "The Burgeoning Molecular Genetics of the Lyme Disease Spirochaete." Nature Reviews Microbiology. 2005. Volume 3(2), p. 129-143.\

Schwan TG, Piesman J, Golde WT, Dolan MC, Rosa PA. "Induction of an outer surface protein on Borrelia burgdorferi during tick feeding." Proceedings of the National Academy of Sciences of the USA. 1995 Mar 28. Volume 92(7). p. 2909-13.

Edited by Dan Holligan, student of Rachel Larsen at UCSD.