SungTimon2.2: Difference between revisions

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If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br>
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br>


Various Test Results:
Various Biochemical Test Results:


Phenol Red Broth Test- Results [Lactose: Pinkish-Red, meaning slightly alkaline product] [Sucrose/Glucose: Yellow-Orange, meaning slightly acidic product] NO GAS (fermentation) produced
NEGATIVE RESULTS INCLUDE: Starch Hydrolysis, Gelatin Hydrolysis, Methyl Red-Voges Proskauer, Citrate test, SIM (negative for sulfur reduction, indole production, nonmotile organism), nitrate reduction, Urea hydrolysis, Eosin methylene blue (no coliforms, lactose fermenters, no growth: meaning possible gram +), Hektoen Enteric (presumptive ID for Gram +), MacConkey Agar (Gram + evidence), Decarboxylation Test, Phenylalanine deaminase, and catalase.


Stach Hydrolysis- Results [Negative, meaning our bacteria does not hydrolyze starch via the use of amylase]
POSITIVE RESULTS INCLUDE: Casein hydrolysis, DNA hydrolysis, Lipid hydrolysis, Triple sugar iron, oxidase, blood agar (beta- complete lysis of RBC), mannitol salt agar (weak positive), and phenylethal alcohol.


Casein Hydrolysis- Results [Positive, meaning our bacteria possesses the enzyme casease in order to hydrolyze the milk protein casein]
Kirby Bauer Antimicrobial and Disinfectant Sensitivity Results:
Strong Zone of Inhibition for linezolid, cefamandole, azlocillin, vancomycin, sufisozazole, clove, and 10% lysol.
Weak Zone of Inhibition for oxacillin, tea tree oil, 100% bleach, and lavender.


Gelatin Hydrolysis- Results [Negative, meaning our bacteria does not hydrolyze gelatin via the use of gelatinase]
Interpretation of Results: Our bacteria is a gram positive organism that possesses the enzymes casease,DNase, ligase, fermentable enzymes, and cytochrome C oxidase. This organism can completely lyse red blood cells, and can weakly ferment mannitol.
 
DNA Hydrolysis- Results [Positive, meaning our bacteria possesses the enzyme DNase in order to catalyze DNA]
 
Lipid Hydrolysis- Results [Positive, meaning our bacteria possesses the enzyme ligase in order to breakdown lipids]
 
Methyl Red-Voges Proskauer: Results [Negative, the organism does not utilize mixed acid fermentation] [Negative, the organism does not reneutralize, meaning the organism does not make acetoin]
 
Citrate Test- Results [The organism does not possess citrate permease, and cannot use citrate as the sole source of carbon]
 
SIM Test (3 in 1)- Results [Sulfur reduction: negative, Indole production for tryptophan: negative, motility: nonmotile]
 
Nitrate Reduction Test- Results [negative for nitrate reducing enzymes]
 
Urea Hydrolysis- Results [negative for the enzyme urease]
 
Triple Sugar Iron Agar- Results [positive for fermentable enzymes] **"orange butt, yellow slant... which we concluded that the butt was not stabbed far enough"
 
Oxidase- Results [positive for the enzyme cytochrome C oxidase]


==References==
==References==

Revision as of 19:08, 16 November 2015

This student page has not been curated.

Classification

Domain; Phylum; Class; Order; family [Others may be used. Use NCBI link to find]

Species

NCBI: Taxonomy

Genus species

Habitat Information

Describe the location and conditions under which the organism was isolated.

Description and Significance

Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.

Genome Structure

Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.


Cell Structure, Metabolism and Life Cycle

Interesting features of cell structure; how it gains energy; what important molecules it produces.


Physiology and Pathogenesis

Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.

Various Biochemical Test Results:

NEGATIVE RESULTS INCLUDE: Starch Hydrolysis, Gelatin Hydrolysis, Methyl Red-Voges Proskauer, Citrate test, SIM (negative for sulfur reduction, indole production, nonmotile organism), nitrate reduction, Urea hydrolysis, Eosin methylene blue (no coliforms, lactose fermenters, no growth: meaning possible gram +), Hektoen Enteric (presumptive ID for Gram +), MacConkey Agar (Gram + evidence), Decarboxylation Test, Phenylalanine deaminase, and catalase.

POSITIVE RESULTS INCLUDE: Casein hydrolysis, DNA hydrolysis, Lipid hydrolysis, Triple sugar iron, oxidase, blood agar (beta- complete lysis of RBC), mannitol salt agar (weak positive), and phenylethal alcohol.

Kirby Bauer Antimicrobial and Disinfectant Sensitivity Results: Strong Zone of Inhibition for linezolid, cefamandole, azlocillin, vancomycin, sufisozazole, clove, and 10% lysol. Weak Zone of Inhibition for oxacillin, tea tree oil, 100% bleach, and lavender.

Interpretation of Results: Our bacteria is a gram positive organism that possesses the enzymes casease,DNase, ligase, fermentable enzymes, and cytochrome C oxidase. This organism can completely lyse red blood cells, and can weakly ferment mannitol.

References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

Author

Page authored by Hsiang-Yuan Sung and Kaleen Timon, student of Prof. Kristine Hollingsworth at Austin Community College.

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