Pseudomonas putida alesha & gwen: Difference between revisions
| Line 39: | Line 39: | ||
==Genome Structure== | ==Genome Structure== | ||
[1] According to the Pseudomonas Genome Database the size of ''Pseudomonas putida KT2440'' genome is 6,181,863 nucleotide pairs. Of that, there is a 61.5% guanine and cytosine pairing and the other 38.5% is adenine and thymine. ''P. putida'' has a single circular chromosome. It is the first gram negative bacteria certified as a biosafety host for cloning of foreign genes [2]. | [1] According to the Pseudomonas Genome Database the size of ''Pseudomonas putida KT2440'' genome is 6,181,863 nucleotide pairs. Of that, there is a 61.5% guanine and cytosine pairing and the other 38.5% is adenine and thymine. ''P. putida'' has a single circular chromosome. ''P. putida'' was found to share many homologous ORF's (4610/5420) with bacterium ''Pseudomonas aeruginosa''. It is the first gram negative bacteria certified as a biosafety host for cloning of foreign genes [2]. Include S Ribosomal sequence that you obtained from PCR and sequencing here. | ||
Gwen | Gwen | ||
Revision as of 19:03, 20 April 2016
Classification
Domain; Phylum; Class; Order; family [Others may be used. Use NCBI link to find]
Domain: Bacteria
Phylum: Proteobacteria
Class: Gammaproteobacteria
Order: Pseudomonadales
Family: Pseudomonadaceae
Genus: Pseudomonas
Species
|
NCBI: Taxonomy |
Genus species
Pseudomonas putida
Habitat Information
Describe the location and conditions under which the organism was isolated.
Alesha
Description and Significance
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.
Alesha
Genome Structure
[1] According to the Pseudomonas Genome Database the size of Pseudomonas putida KT2440 genome is 6,181,863 nucleotide pairs. Of that, there is a 61.5% guanine and cytosine pairing and the other 38.5% is adenine and thymine. P. putida has a single circular chromosome. P. putida was found to share many homologous ORF's (4610/5420) with bacterium Pseudomonas aeruginosa. It is the first gram negative bacteria certified as a biosafety host for cloning of foreign genes [2]. Include S Ribosomal sequence that you obtained from PCR and sequencing here.
Gwen
Cell Structure, Metabolism and Life Cycle
Interesting features of cell structure; how it gains energy; what important molecules it produces.
Gwen
Physiology and Pathogenesis
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.
Alesha http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.0020007
References
[1] Winsor GL, Griffiths EJ, Lo R, Dhillon BK, Shay JA, Brinkman FS (2016). Pseudomonas Genome DB. The Brinkman Lab at Simon Fraser University. n.d. Web. 20 April 2016. <http://www.pseudomonas.com/strain/show/110>
[2] Nelson KE, Weinel C, Paulsen IT, Dodson RJ, Hilbert H, et al. (2002) Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440. Environ Microbiol 4: 799–808.
Author
Page authored by _____, student of Prof. Kristine Hollingsworth at Austin Community College.
