Pseudomonas putida alesha & gwen

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Classification

Domain; Phylum; Class; Order; family [Others may be used. Use NCBI link to find]

Domain: Bacteria

Phylum: Proteobacteria

Class: Gammaproteobacteria

Order: Pseudomonadales

Family: Pseudomonadaceae

Genus: Pseudomonas

Species

Pseudomonas putida

NCBI: Taxonomy

Habitat Information

Describe the location and conditions under which the organism was isolated.

Alesha

Description and Significance

Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.

Alesha

Genome Structure

[1] According to the Pseudomonas Genome Database the size of Pseudomonas putida KT2440 genome is 6,181,863 nucleotide pairs. Of that, there is a 61.5% guanine and cytosine pairing and the other 38.5% is adenine and thymine. P. putida has a single circular chromosome. P. putida was found to share many homologous ORF's (4610/5420) with bacterium Pseudomonas aeruginosa. It is the first gram negative bacteria certified as a biosafety host for cloning of foreign genes [2]. Include S Ribosomal sequence that you obtained from PCR and sequencing here.

Gwen

Cell Structure, Metabolism and Life Cycle

Pseudomonas putida is a gram negative rod shaped bacteria. It is nonsporeforming and is motile by one or more flagella. ; how it gains energy; what important molecules it produces.

Gwen

Physiology and Pathogenesis

Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.

Alesha http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.0020007

References

[1] Winsor GL, Griffiths EJ, Lo R, Dhillon BK, Shay JA, Brinkman FS (2016). Pseudomonas Genome DB. The Brinkman Lab at Simon Fraser University. n.d. Web. 20 April 2016. <http://www.pseudomonas.com/strain/show/110>

[2] Nelson KE, Weinel C, Paulsen IT, Dodson RJ, Hilbert H, et al. (2002) Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440. Environ Microbiol 4: 799–808.

Author

Page authored by Alesha Larkins and Gwen Schurmann, student of Prof. Kristine Hollingsworth at Austin Community College.